A5100432116- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Immune cells in cancer
- MicroRNA in disease regulation
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Extracellular vesicles in disease
- Cancer Immunotherapy and Biomarkers
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- interferon and immune responses
- RNA Research and Splicing
- IL-33, ST2, and ILC Pathways
- Cancer-related gene regulation
- Aquaculture disease management and microbiota
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Viral Infections and Vectors
- Vibrio bacteria research studies
- Genomics and Phylogenetic Studies
- Histone Deacetylase Inhibitors Research
Sichuan University
2024-2025
Third Affiliated Hospital of Sun Yat-sen University
2025
Sun Yat-sen University
2013-2025
Jinan University
2025
Chinese University of Hong Kong
2023-2025
Tianjin Medical University Cancer Institute and Hospital
2017-2024
Tianjin University of Traditional Chinese Medicine
2018-2024
Nanjing Medical University
2011-2024
Jiangsu Province Hospital
2011-2024
Jilin University
2019-2024
We report here the identification and characterization of a protein, ERIS, an endoplasmic reticulum (ER) IFN stimulator, which is strong type I stimulator plays pivotal role in response to both non-self-cytosolic RNA dsDNA. ERIS (also known as STING or MITA) resided exclusively on ER membrane. The retention/retrieval sequence RIR was found be critical retain protein membrane maintain its integrity. dimerized innate immune challenges. Coumermycin-induced dimerization led fast induction,...
Abstract The invasion of malignant glioma cells into the surrounding normal brain tissues is crucial for causing poor outcome this tumor type. Recent studies suggest that stem-like (GSLCs) mediate invasion. However, it not clear whether microenvironment factors, such as tumor-associated microglia/macrophages (TAM/Ms), also play important roles in promoting GSLC In study, we found primary human gliomas and orthotopical transplanted syngeneic glioma, number TAM/Ms at invasive front was...
Circular RNAs (circRNAs) are a class of non-coding with loop structure, but its functions remain largely unknown. Growing evidence has revealed that circRNAs play striking role as functional in the progression malignant disease. However, precise gastric cancer (GC) remains unclear.CircRNAs were determined by human circRNA array analysis and quantitative reverse transcription polymerase reaction. Luciferase reporter, RNA pull down, fluorescence situ hybridization assays employed to test...
Abstract Treating large established tumors is challenging for dendritic cell (DC)-based immunotherapy. DC activation with tumor cell-derived exosomes (TEXs) carrying multiple tumor-associated antigen can enhance recognition. Adding a potent adjuvant, high mobility group nucleosome-binding protein 1 (HMGN1), boosts DCs’ ability to activate T cells and improves vaccine efficiency. Here, we demonstrate that TEXs painted the functional domain of HMGN1 (TEX-N1ND) via an exosomal anchor peptide...
Liver metastasis (LM) is a major obstacle to the prognosis of gastric cancer (GC) patients, but molecular mechanism underlying liver (GC-LM) remains unknown. Exosomes have been identified as an important mediator communication between tumor cells and microenvironment. Therefore, we sought investigate effects primary GC on microenvironment role exosomal microRNAs (exo-miRNA) in GC-LM.Sequential differential centrifugation, transmission electron microscopy NanoSight analysis were used extract...
Abstract Abnormal 5-methylcytosine (m5C) methylation has been proved to be closely related gastric carcinogenesis, progression, and prognosis. Dysregulated long noncoding RNAs (lncRNAs) participate in a variety of biological processes cancer. However, date, m5C-methylated lncRNAs are rarely researched cancer (GC). Here, we found that RNA cytosine-C(5)-methyltransferase (NSUN2) was upregulated GC high NSUN2 expression associated with poor NR_033928 identified as an NSUN2-methylated lncRNA GC....
PTEN is a tumor suppressor frequently mutated in cancer. Recent reports implicated Nedd4-1 as the E3 ubiquitin ligase for that regulates its stability and nuclear localization. We tested physiological role of regulator by using cells tissues derived from two independently generated strains mice with their gene disrupted. ubiquitination were indistinguishable between wild-type Nedd4-1-deficient cells, an interaction proteins could not be detected. Moreover, subcellular distribution, showing...
The rapid development of CRISPR/Cas9 systems has opened up tantalizing prospects to sensitize cancers chemotherapy using efficient targeted genome editing, but safety concerns and possible off-target effects viral vectors remain a major obstacle for clinical application. Thus, the construction novel nonviral tumor-targeting nanodelivery great potential safe application gene–chemo-combination therapy. Here, we report polyamidoamine-aptamer-coated hollow mesoporous silica nanoparticle...
Abstract BACKGROUND Reliable noninvasive biomarkers for hepatocellular carcinoma (HCC) diagnosis and prognosis are urgently needed. We explored the potential of not only microRNAs (miRNAs) but other types noncoding RNAs (ncRNAs) as HCC biomarkers. METHODS Peripheral blood samples were collected from 77 individuals; among them, 57 plasma cell-free RNA transcriptomes 20 exosomal profiled. Significantly upregulated ncRNAs published validated with reverse transcription (RT)-qPCR in an...
N6-methyladenosine (m6A) is capable of mediating circRNA generation in carcinoma biology. Nevertheless, the posttranscriptional systems m6A and hepatocellular (HCC) development are still unclear. The present study identified a with modification, circHPS5, which was increased neoplasm HCC tissues indicated poor patient survival. Silencing circHPS5 inhibited epithelial-mesenchymal transition (EMT) cancer stem-like cell (CSC) phenotypes. Notably, METTL3 could direct formation specific...
Circular RNAs (circRNAs) play vital regulatory roles in the progression of multiple cancers. In our study, transcriptome analysis and self-organizing maps (SOM) were applied to screen backbone circRNAs gastric cancer (GC). Upon validation expression patterns screened circRNAs, gain- loss-of-function assays performed vitro vivo. Underlying mechanisms investigated using RNA pull-down, luciferase reporter assay immunoprecipitation. The circTHBS1 was significantly increased GC associated with...
Abstract Oxaliplatin resistance inevitably occurs in almost all cases of metastatic colorectal cancer (CRC), and it is important to study the roles lncRNAs their specific regulatory mechanisms oxaliplatin resistance. Exosomes are increasingly designed for drug or functional nucleic acid delivery due properties, thereby improving effectiveness therapy. The results this show that low expression PGM5 antisense RNA 1 (PGM5‐AS1) colon induced by transcription inhibitor, GFI1B. PGM5‐AS1 prevents...
Neural invasion (NI) is a vital pathological characteristic of gastric cancer (GC), which correlates with tumor recurrence and worse prognosis. Long noncoding RNAs (lncRNAs) play critical roles in various biological processes. However, the involvement lncRNAs NI GC (GC-NI) remains unclear. DIAPH2-AS1 was upregulated NI-positive tissues, confirmed by qRT-PCR. The higher expression predicted survival for patients. Both vitro vivo experiments, including wound-healing assay, Transwell DRG-GC...
Abstract Diseases like obesity and intestinal inflammation diseases are accompanied by dysbiosis of the gut microbiota (DSGM), which leads to various complications, including systemic metabolic disorders. DSGM reportedly impairs fertility male mice; however, regulatory mechanism is unclear. Exosomes molecular mediators intercellular communication, but regulation spermatogenesis non‐reproductive tissue‐originated exosomes remains unknown. The present study shows that altered miRNA expression...
MicroRNA-223-3p (miR-223-3p) is one of the potential microRNAs that have been shown to alleviate inflammatory responses in pre-clinical investigations and highly encased exosomes derived from bone mesenchymal stem cells (MSC-exosomes). MSC-exosomes are able function as carriers deliver into cells. Autoimmune hepatitis challenging liver diseases with no effective treatment other than steroid hormones. Here, we examined whether can transfer miR-223-3p treat autoimmune an experimental model. We...
Granulosa cells (GCs) are regulated by various factors during ovarian development. However, there few reports on the role of follicular fluid exosomes in GCs. In this study, porcine GCs were used to explore effects treated with vitro, and changes cell proliferation, steroid synthesis, associated signal pathways detected. The results showed that increased viability altered gene expression profile Exosomes also level both proliferation progesterone which MAPK/ERK WNT/B-CATENIN involved....