A5101941661- Synthesis of Indole Derivatives
- Carbohydrate Chemistry and Synthesis
- Nanoparticle-Based Drug Delivery
- Bioactive Compounds and Antitumor Agents
- Kruppel-like factors research
- Ultrasound and Cavitation Phenomena
- Photoacoustic and Ultrasonic Imaging
- Ultrasound and Hyperthermia Applications
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Genetic Syndromes and Imprinting
- Immunotherapy and Immune Responses
- Synthesis and Biological Evaluation
- Chemical Synthesis and Analysis
- Cancer therapeutics and mechanisms
- MicroRNA in disease regulation
- Proteoglycans and glycosaminoglycans research
- Extracellular vesicles in disease
- Natural product bioactivities and synthesis
- Influenza Virus Research Studies
- Click Chemistry and Applications
- Blood Coagulation and Thrombosis Mechanisms
- Nanocluster Synthesis and Applications
- Cancer, Hypoxia, and Metabolism
- Wireless Communication Security Techniques
Institute of Basic Medical Sciences of the Chinese Academy of Medical Sciences
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2009-2023
MRC Laboratory of Molecular Biology
2023
Huazhong University of Science and Technology
2013-2022
Hunan Institute of Mental Health
2022
Second People Hospital of Hunan
2021
National Engineering Research Center for Nanotechnology
2019
Virginia Commonwealth University
2005-2016
Wuhan No.1 Hospital
2015
Hong Kong Polytechnic University
1999-2009
An exosomal anchor peptide enables targeting, drug loading, and capture of exosomes from diverse origins targets oligonucleotides to muscle in mdx mice.
Abstract Treating large established tumors is challenging for dendritic cell (DC)-based immunotherapy. DC activation with tumor cell-derived exosomes (TEXs) carrying multiple tumor-associated antigen can enhance recognition. Adding a potent adjuvant, high mobility group nucleosome-binding protein 1 (HMGN1), boosts DCs’ ability to activate T cells and improves vaccine efficiency. Here, we demonstrate that TEXs painted the functional domain of HMGN1 (TEX-N1ND) via an exosomal anchor peptide...
Personalized immunotherapy utilizing cancer vaccines tailored to the tumors of individual patients holds promise for with high genetic heterogeneity, potentially enabling eradication tumor in its entirety.Here, we demonstrate a general strategy biological nanovaccines that trigger tumor-specific immune responses hepatocellular carcinoma (HCC). Dendritic cell (DC)-derived exosomes (DEX) are painted HCC-targeting peptide (P47-P), an α-fetoprotein epitope (AFP212-A2) and functional domain...
Abstract Superparamagnetic iron oxide nanoparticles (SPIONs) as a contrast agent have been widely used in magnetic resonance imaging for tumor diagnosis and theranostics. However, there has safety concern of SPIONs with cirrhosis related to excess iron-induced oxidative stress. In this study, the impact overload by was assessed on mouse model. A single dose SPION injection at 0.5 or 5 mg Fe/kg group induced septic shock response 24 h elevated serum levels liver kidney function markers...
Selective alkylation of a chosen sequence DNA typically relies on ligand-directed delivery compound that expresses an intrinsic reactivity. A significant and biologically relevant enhancement in specificity is theoretically possible if such reactivity could be replaced by latent activity induced solely the target interest, but examples this are rare not easily emulated. simple strategy for target-promoted now illustrated intramolecular adduct formed oligonucleotide–quinone methide conjugate....
Thrombin is a key enzyme targeted by the majority of current anticoagulants that are direct inhibitors. Allosteric inhibition thrombin may offer major advantage finely tuned regulation. We present here sulfated benzofurans as first examples potent, small allosteric inhibitors thrombin. A benzofuran library 15 monomers and 13 dimers with different charged, polar, hydrophobic substituents was studied in this work. Synthesis achieved through multiple step, highly branched strategy, which...
We recently designed a group of novel exosite-2-directed sulfated, small, allosteric inhibitors thrombin. To develop more potent inhibitors, monosulfated benzofuran tri- and tetrameric homologues the parent dimers were synthesized in seven to eight steps found exhibit wide range potencies. Among these, trimer 9a was be nearly 10-fold than first generation molecules. Michaelis-Menten studies indicated an mechanism inhibition. Competitive using hirudin peptide (exosite 1 ligand) unfractionated...
Superparamagnetic iron oxide nanoparticles (SPIONs) have recently been used as an effective magnetic resonance imaging (MRI) contrast agent for the noninvasive diagnosis of chronic liver diseases including nonalcohol fatty diseases, steatohepatitis, and cirrhosis well tumors. However, potential risk overload by SPIONs has highly underestimated in diseases. While most shown safe healthy group, significant toxicity revealed through immunotoxicity, lipid peroxidation, acid cholesterol...
Cells are incessantly exposed to many sources of genotoxic stress. A critical unresolved issue is how the resulting activation p53 tumor suppressor can lead either cell cycle arrest or apoptosis depending on extent DNA damage. The present study shows that level Krüppel-like factor 4 (KLF4) expression inversely correlated with KLF4 activated by following cytostatic, mild damage, whereas it strongly repressed via enhanced turnover mRNA severe damage irreversibly drives cells apoptosis....
Nanobubbles and microbubbles are non-invasive ultrasound imaging contrast agents that may potentially enhance diagnosis of tumors. However, to date, both nanobubbles display poor in vivo tumor-selectivity over non-targeted organs such as liver. We report here cyanine 5.5 conjugated (cy5.5-nanobubbles) a biocompatible chitosan–vitamin C lipid system dual ultrasound-fluorescence agent achieved tumor-selective mouse tumor model. Cy5.5-nanobubble suspension contained single bubble spheres...
Matrix protein 1 (M1) of the influenza A virus plays multiple roles in virion assembly and infection. Interest pH dependence M1's functions led us to study effect subtle changes on M1 structure, resulting elucidation a unique low-pH crystal structure N(1-165)-domain A/WSN/33 (H1N1) that has never been reported. Although 2.2 Å N-terminus shows dimer with two monomers interacting face-to-face fashion at low as observed earlier, 44° rotation second monomer significantly different interface...
Earlier, we reported on the design of sulfated benzofuran dimers (SBDs) as allosteric inhibitors thrombin (Sidhu et al. J. Med. Chem.201154 5522-5531). To identify site binding SBDs, studied inhibition in presence exosite 1 and 2 ligands. Whereas hirudin peptide heparin octasaccharide did not affect IC(50) by a high affinity SBD, full-length reduced potency 4-fold. The γ' fibrinogen peptide, which recognizes Arg93, Arg97, Arg173, Arg175, other residues, resulted loss that correlated with...
Inflammatory responses by kidney mesangial cells play a critical role in the glomerulonephritis. The anti-inflammatory potential of nineteen mono-, di- and polyhydroxylated flavones including fisetin, quercetin, morin, tricetin, gossypetin, apigenin myricetin were investigated on rat with lipopolysaccharide (LPS) as inflammatory stimuli. 6-Hydroxyflavone 4′,6-dihydroxyflavone exhibited high activity IC50 range 2.0 μM, much better inhibition comparison to well-studied flavones. Interestingly,...
The authors investigate a cooperative network with three terminals. By exploiting simple two-bit feedback message from the destination, two incremental relaying protocols are proposed, namely selection amplify-and-forward (ISAF) and joint (JISR) an aim to balance load between source relay. derive asymptotic outage probabilities of new find them be lower than that (IAF) protocol, which has been identified as best protocol so far. Moreover, spectral efficiencies ISAF JISR match IAF. Simulation...
Summary Recently we prepared sulfated, low-molecular-weight lignins (LMWLs) to mimic the biological activities of heparin and heparan sulfate. Chemo-enzymatically sulfated LMWLs represent a library diverse non-sugar, aromatic molecules with structures radically different from heparins, have been found potently inhibit thrombin factor Xa. To assess their effect on fibrinolytic system, studied interaction human plasmin. Enzyme inhibition studies indicate that three plasmin IC50 values in range...
Quinone methides (QMs) are involved in the metabolism of many drugs and carcinogens as reactive intermediates to form covalent nucleobase adducts DNA that associate with high mutagenicity. Recently, a plethora synthetic QM alkylating agents have been developed various potential antitumor agents. However, mutagenic these has not fully investigated. In this report, N-methylquinolinium was model study biological consequences formation target. N-Methylquinolinium generated situ via an...
Although superparamagnetic iron oxide (SPIO) nanoparticles have been developed as a contrast agent for magnetic resonance imaging (MRI), acute overload due to the persistently high retention of SPIOs in liver and spleen that are slowly converted ferroproteins is serious safety concern. Here, we report addition poly-L-lysine polymers an SPIO hydroxyethyl starch solution produced tightly controlled, monodispersed size-dependent manner effective agents MRI tumors. High was demonstrated with...
Thymidine kinases (TKs) have been considered one of the potential targets for anticancer therapeutic because their elevated expressions in cancer cells. However, nucleobase analogs targeting TKs shown poor selective cytotoxicity cells despite effective antiviral activity. 3'-Deoxythymidine phenylquinoxaline conjugate (dT-QX) was designed as a novel analog to target and block cell replication via conjugated DNA intercalating quinoxaline moiety. In vitro screening showed that dT-QX selectively...