A5054868980- Connexins and lens biology
- Alzheimer's disease research and treatments
- Neurogenesis and neuroplasticity mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Biochemical effects in animals
- Barrier Structure and Function Studies
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- RNA Research and Splicing
- Adipose Tissue and Metabolism
- Fatty Acid Research and Health
- Birth, Development, and Health
- Genetics and Neurodevelopmental Disorders
- S100 Proteins and Annexins
- MicroRNA in disease regulation
- Photoreceptor and optogenetics research
- Pain Mechanisms and Treatments
- Cytokine Signaling Pathways and Interactions
- Diet and metabolism studies
- Signaling Pathways in Disease
- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Neonatal and fetal brain pathology
Sun Yat-sen University
2019-2025
The Seventh Affiliated Hospital of Sun Yat-sen University
2019-2025
Guangdong 999 Brain Hospital
2023-2025
Army Medical University
2024-2025
First Affiliated Hospital of Gannan Medical University
2023
Key Laboratory of Guangdong Province
2023
National University of Singapore
2020-2022
Centre Interdisciplinaire de Recherche en Biologie
2015-2018
Inserm
1982-2018
Collège de France
2015-2018
Alzheimer's disease is a neurodegenerative disorder that causes age-dependent neurological and cognitive declines. The treatments for pose significant challenge, because the mechanisms of are not being fully understood. Malfunction blood-brain barrier increasingly recognized as major contributor to pathophysiology disease, especially at early stages disease. However, underlying remain poorly characterized, while few molecules can directly target improve function in context Here, we showed...
Astrocytes interact dynamically with neurons by modifying synaptic activity and plasticity. This interplay occurs through a process named gliotransmission, meaning that neuroactive molecules are released astrocytes. Acting as gliotransmitter, D-serine, co-agonist of the NMDA receptor at glycine-binding site, can be astrocytes in calcium [Ca 2+ ] i -dependent manner. A typical feature is their high expression level connexin43 (Cx43), protein forming gap junction channels hemichannels...
Abstract The contribution of reactive gliosis to the pathological phenotype Alzheimer's disease (AD) opened way for therapeutic strategies targeting glial cells instead neurons. In such context, connexin hemichannels were proposed recently as potential targets since neuronal suffering is alleviated when expression genetically suppressed in astrocytes a murine model AD. Here, we show that boldine, an alkaloid from boldo tree, inhibited hemichannel activity and microglia without affecting gap...
The precise contribution of astrocytes in neuroinflammatory process occurring Parkinson's disease (PD) is not well characterized. In this study, using GRCx30CreERT2 mice that are conditionally inactivated for glucocorticoid receptor (GR) astrocytes, we have examined the actions astrocytic GR during dopamine neuron (DN) degeneration triggered by neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). results show significantly augmented DN loss mutant substantia nigra (SN) compared to...
Oligodendrocyte precursor cells (OPCs) undergo an extensive and coordinated migration in the developing CNS, using pre-formed scaffold of developed blood vessels as their physical substrate for migration. While OPC association with vasculature is critical dispersal, equally important permitting differentiation proper myelination target axons appropriate timely detachment, but regulation this process remains unclear. Here we demonstrate a correlation between developmental formation astrocytic...
Abstract Although the link of white matter to pathophysiology schizophrenia is documented, loss myelin not detected in patients at early stages disease, suggesting that pathological evolution may occur before significant loss. Disrupted-in-schizophrenia-1 (DISC1) protein highly expressed oligodendrocyte precursor cells (OPCs) and regulates their maturation. Recently, DISC1-Δ3, a major DISC1 variant lacks exon 3, has been identified patients, although its significance remains unknown. In this...
Most patients with stroke caused by middle cerebral artery occlusion (MCAO) show cognitive deficit that is generally regarded as resulting from damage to the cortex rather than hippocampus. Whether MCAO induces hippocampal and whether this contributes defects remains unclear. Here we investigate its correlation after exclusively unilateral underlying mechanism for damage.Patients were assessed magnetic resonance imaging (MRI) angiography (MRA), Mini Mental-Status Evaluation (MMSE) Rey...
An emergent concept in neurosciences consists considering brain functions as the product of dynamic interactions between neurons and glial cells, particularly astrocytes. Although role played by astrocytes synaptic transmission plasticity is now largely documented, their contribution to neuronal network activity only beginning be appreciated. In mouse olfactory bulb slices, we observed that membrane potential mitral cells oscillates UP DOWN states at a low frequency (<1 Hz). Such slow...
ABSTRACT Oligodendrocyte precursor cells (OPCs) undergo a series of energy-consuming developmental events; however, the uptake and trafficking pathways for their energy metabolites remain unknown. In present study, we found that 2-NBDG, fluorescent glucose analog, can be delivered between astrocytes oligodendrocytes through connexin-based gap junction channels but cannot transferred OPCs. Instead, connexin hemichannel-mediated supports OPC proliferation, ethidium bromide or increase 2-NBDG...
Mast cells (MCs) store an array of proinflammatory mediators in secretory granules that are rapidly released upon activation by diverse conditions including amyloid beta (Aβ) peptides. In the present work, we found a rapid degranulation cultured MCs through pannexin1 hemichannel (Panx1 HC)-dependent mechanism induced Aβ<sub>25–35</sub> peptide. Accordingly, peptide also increased membrane current and permeability, as well intracellular Ca<sup>2+</sup> signal, mainly via Panx1 HCs because all...
Abstract The impairment of the blood-brain barrier (BBB) has been increasingly recognised as a critical element in early pathogenesis Alzheimer’s disease (AD), prompting focus on brain endothelial cells (BECs), which serve primary constituents BBB. Death receptor 6 (DR6) is highly expressed vasculature and acts downstream Wnt/β-catenin pathway to promote BBB formation during development. Here, we found that DR6 levels were significantly reduced murine model AD (APP swe /PS1 dE9 mice) at...
Abstract Oligodendrocyte precursor cells (OPCs) shape brain function through many non-canonical regulatory mechanisms beyond myelination. Here we show that OPCs form contacts with their processes on neuronal somata in a activity-dependent manner. These facilitate exocytosis of lysosomes. A reduction the number or branching reduces these contacts, which is associated lysosome accumulation and altered metabolism neurons more senescent age. similar OPC seen an early-stage mouse model...
Recently, ligand-promoted Au(I)/Au(III)-catalyzed cross-coupling reactions with aryl iodides have garnered considerable attention. Here, we report the first visible-light-driven gold-catalyzed cross-couplings of challenging bromides. In presence a (P, N)-gold(I) catalyst and an acridinium photocatalyst under blue LED irradiation, C-O coupling bromides carboxylic acids was achieved, soon it found that this photoinduced appliable for other C-C, C-N, C-S bond formation. Experimental...
Indoor vaping can lead to third-hand vapour exposure, evidenced by the presence of nicotine and carcinogenic nitrosamines on indoor surfaces. Children are at high risk such exposure. This study aimed investigate effects exposure cognitive function in young mice. Male mice (Balb/c, 4 weeks) were exposed cotton towels treated with e-cigarette without (9 L chamber filled 20 puffs for 2 hours) changed daily four weeks. Vapour was generated from tobacco-flavoured liquids (50 % propylene glycol,...
Reproducing the pathophysiology of human multiple sclerosis (MS) in animal models is critical to identifying mechanisms triggering demyelination and developing early intervention strategies. Here, we aimed model overactivated Wnt (wingless-related integration site) signaling previously shown postmortem brain tissues patients with MS by inducing experimental autoimmune encephalomyelitis (EAE) Pdgfra CreER ;Apc fl/fl Olig2 Cre mice. These mice have oligodendrocyte precursor cells (OPCs)...
Astrocytes represent a major non‐neuronal cell population actively involved in brain functions and pathologies. They express large amount of gap junction proteins that allow communication between adjacent glial cells the formation networks. In addition, these membrane can also operate as hemichannels, through which “gliotransmitters” are released, thus contribute to neuroglial interaction. There now reports demonstrating alterations astroglial and/or hemichannel activity impact neuronal...