A5072725666- Marine Ecology and Invasive Species
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- Marine Biology and Environmental Chemistry
- Crustacean biology and ecology
- Developmental Biology and Gene Regulation
- Marine Bivalve and Aquaculture Studies
- Environmental DNA in Biodiversity Studies
- Physiological and biochemical adaptations
- Genomics and Phylogenetic Studies
- Coral and Marine Ecosystems Studies
- Protist diversity and phylogeny
- Studies on Chitinases and Chitosanases
- Marine Biology and Ecology Research
- MicroRNA in disease regulation
- Genetic diversity and population structure
- Single-cell and spatial transcriptomics
- Marine animal studies overview
- Biodiesel Production and Applications
- Genetic Mapping and Diversity in Plants and Animals
- interferon and immune responses
- Hearing, Cochlea, Tinnitus, Genetics
- Aldose Reductase and Taurine
- Identification and Quantification in Food
- Cancer-related molecular mechanisms research
Ocean University of China
2017-2024
Qingdao National Laboratory for Marine Science and Technology
2019-2024
Laoshan Laboratory
2022-2023
Chinese University of Hong Kong
2020
University of Chinese Academy of Sciences
2013-2016
Institute of Oceanology
2013-2015
Chinese Academy of Sciences
2013-2015
Penaeid shrimp has a distinctive metamorphosis stage during early development. Although morphological and biochemical studies about this ontogeny have been developed for decades, researches on gene expression level are still scarce. In study, we investigated the transcriptomes of five continuous developmental stages in Pacific white (Litopenaeus vannamei) with high throughput Illumina sequencing technology. The reads were assembled clustered into 66,815 unigenes, which 32,398 putative...
Molting is one of the most important biological processes in shrimp growth and development. All undergo cyclic molting periodically to shed replace their exoskeletons. This process essential for growth, metamorphosis, reproduction shrimp. However, molecular mechanisms underlying remain poorly understood. In this study, we investigated global expression changes transcriptomes Pacific white shrimp, Litopenaeus vannamei, commonly cultured species worldwide. The transcriptome whole L. vannamei...
The application of next generation sequencing technology has greatly facilitated high throughput single nucleotide polymorphism (SNP) discovery and genotyping in genetic research. In the present study, SNPs were discovered based on two transcriptomes Litopenaeus vannamei (L. vannamei) generated from Illumina platform HiSeq 2000. One transcriptome L. was obtained through RNA larvae at mysis stage its reference sequence de novo assembled. data another downloaded NCBI reads mapped separately to...
Crustacea, particularly Decapoda, contains many economically important species, such as shrimps and crabs. Crustaceans exhibit enormous (nearly 500-fold) variability in genome size. However, limited resources are available for investigating these species. Exopalaemoncarinicauda Holthuis, an economical caridean shrimp, is a potential ideal experimental animal research on crustaceans. In this study, we performed low-coverage sequencing de novo assembly of the E. carinicauda genome. The covers...
Exoskeleton construction is an important issue in shrimp. To better understand the molecular mechanism of exoskeleton formation, development and reconstruction, transcriptome entire developmental process Litopenaeus vannamei, including nine early stages eight adult-moulting stages, was sequenced analysed using Illumina RNA-seq technology. A total 117,539 unigenes were obtained, with 41.2% predicting full-length coding sequence. Gene Ontology, Clusters Orthologous Group (COG), Kyoto...
Abstract Zygotic genome activation (ZGA) is a universal process in early embryogenesis of metazoan, when the quiescent zygotic nucleus initiates global transcription. However, mechanisms related to massive and allele-specific expression (ASE) remain not well understood. Here, we develop hybrids from two deeply diverged (120 Mya) ascidian species symmetrically document dynamics ZGA. We identify coordinated ZGA waves represent developmental housekeeping gene reactivation, respectively....
Tunicates occupy the evolutionary position at boundary of invertebrates and vertebrates. It exhibits adaptation to broad environmental conditions is distributed globally. Despite hundreds years embryogenesis studies, genetic basis invasive habits ascidians remains largely unknown. The leathery sea squirt, Styela clava, an important species. We used chromosomal-level genome transcriptome S. clava explore its genomic- molecular-network-based mechanisms environments. Compared with Ciona...
The pharyngeal endoderm, an innovation of deuterostome ancestors, contributes to development by influencing the patterning and differentiation structures in vertebrates; however, evolutionary origin organs vertebrates is largely unknown. endostyle, a distinct organ exclusively present basal chordates, represents good model for understanding origins. Using Stereo-seq single-cell RNA sequencing, we constructed aspatially resolved atlas endostyle ascidian Styela clava . We determined cell...
Abstract Cell morphology and tissue integrity are essential for embryogenesis. Caveolins membrane proteins that induce the formation of surface pits called caveolae serve as reservoirs cell protection during development. In vertebrates, caveolin 1 (Cav1) 3 (Cav3) required caveola formation. However, function caveolins in invertebrates largely unknown. this study, three caveolins, Cav‐a , Cav‐b CavY identified genome invertebrate chordate Ciona spp. Based on phylogenetic analysis, is found to...
Abstract Background Gut microbiota plays important roles in host animal metabolism, homeostasis and environmental adaptation. However, the interplay between gut microbiome urochordate ascidian, most closet relative of vertebrate, remains less explored. In this study, we characterized microbial communities ascidian ( Halocynthia roretzi ) across changes season starvation stress using a comprehensive set omic approaches including 16S rRNA gene amplicon sequencing, shotgun metagenomics,...
Evolutionary developmental biology (evo-devo) has been among the most fascinating interdisciplinary fields for decades, which aims to elucidate origin and evolution of diverse processes. The rapid accumulation omics data provides unprecedented opportunities answer many interesting but unresolved evo-devo questions. However, access utilization these resources are hindered by challenges particularly in non-model animals. Here, we establish a comparative multi-omics database animal (EDomics,...
The extracellular matrix (ECM) not only provides essential physical scaffolding for cellular constituents but also initiates crucial biochemical and biomechanical cues that are required tissue morphogenesis. In this study, we utilized wheat germ agglutinin (WGA) staining to characterize the ECM architecture in ascidian embryos larvae. results showed three distinct populations of presenting Ciona embryogenesis: outer layer localized at surface embryo, an inner notochord sheath apical secreted...
Abstract Background In recent years, as the development of next-generation sequencing technology, a growing number genes have been reported being horizontally transferred from prokaryotes to eukaryotes, most them involving arthropods. As member phylum Arthropoda, Pacific white shrimp Litopenaeus vannamei has adapt complex water environments with various symbiotic or parasitic microorganisms, which provide platform for horizontal gene transfer (HGT). Results this study, we analyzed...
Metamorphosis takes place within the life cycle of most marine invertebrates. The ascidian is a classical model to study complex cellular processes and underlying molecular mechanisms involved in its larval metamorphosis. detailed signaling pathways remain elusive, though extracellular signal-regulated kinases (ERKs) c-Jun N-terminal kinase (JNK) have been revealed regulate cell migration, differentiation, apoptosis organ regression juvenile development. MicroRNAs (miRNAs) are small...
The endostyle is a pharyngeal organ with an opening groove and cilia in invertebrate chordates (amphioxus ascidian) cyclostomate (lamprey), serving as filter-feeding tract thyroid-secreting location. Emerging evidence implies its complex cellular composition potentially versatile functions. Multiple cell types the have been thought to be progenitors of organs advanced vertebrates. To describe expression profile potential functions, bulk RNA sequencing on ascidian Styela clava was conducted...
Osmoregulation is essential for organisms to adapt the exterior environment and plays an important role in embryonic organogenesis. Tubular organ formation usually involves a hyperosmotic lumen environment. The mechanisms of how cells respond regulate remain largely unknown. Here, we reported that nuclear factor activated T cells-5 (NFAT5), only transcription NFAT family involved cellular responses hypertonic stress, regulated notochord chordate Ciona. Ciona NFAT5 (Ci-NFAT5) was expressed...
Tunicates include diverse species, as they are model animals for evolutionary developmental biology study. The embryonic development of tunicates is known to be extensively regulated by transcription factors (TFs). Styela clava, the globally distributed invasive tunicate, exhibits a strong capacity environmental adaptation. However, TFs were not systematically identified and analyzed. In this study, we reported 553 categorized into 60 families from S. based on whole genome data. Comparison...
Abstract It has long been known that abundant symbiotic bacteria exist in the tunic and gut of marine ascidians, these play crucial roles host development, physiological metabolism, environmental adaptation. However, identity, functions are for only a few strains. In this study, we isolated cultivated 263 strains microorganisms from intestine ascidian Halocynthia roretzi through combination aerobic anaerobic culture approaches. Most species, both anaerobic, stool samples belonged to genus...