Summary. The proteins of epididymal luminal fluid and spermatozoa recovered from different regions the rat epididymis were examined by polyacrylamide gel electrophoresis under non-denaturing denaturing conditions. Albumin (A) four major pre-albumin bands (B–E) observed in cauda on gels. By comparing migration these with that standard globular gels, molecular weight Bands B C was estimated to be 16 000, Band D 30 000 E 32 000. apparently glycoproteins since they stained periodic acid–...

Abstract Background Frailty is a multidimensional vulnerability resulting from age-associated decline. The impact of frailty on outcomes was assessed in cohort vascular surgical patients. Methods study included patients aged over 65 years with length hospital stay (LOS) greater than 2 days, who were admitted to tertiary unit single calendar year. Demographics, mode admission, diagnosis, mortality, LOS and discharge destination recorded, as well variety frailty-specific characteristics. LOS,...

cDNA clones coding for two closely related androgen‐dependent sperm‐coating glycoproteins secreted by the rat epididymis were selected screening an epididymal library constructed in λgt 11 with affinity‐purified antibody directed against glycoproteins. The largest clone of 956 nucleotides provided information a protein 246 amino acids which first 19 residues comprise putative signal peptide sequence when cleaved would produce mature 227 and molecular mass 26 kDa. Confirmation identity was...

1192 and social problems can interact in end- lessly complex ways to produce psychiatric disturbance, ones sympathy has go the Editor trying offer a satisfactory chapter of conclusion this book.One should perhaps not be disappointed that he failed, but grateful so many basic scientists clinicians are working area.

Radioactive carnitine, in the form of L-[methyl-3H]carnitine, was administered intravenously to male rats and specific radioactivity carnitine blood plasma 13 tissues measured for 16 days. There no evidence metabolism other compounds. A compartmental analysis made by comparing variation with time each tissue one two models. Kidney, heart epididymal fat were best represented as containing a single compartment whereas spleen, liver, lung, adrenal, prostate, seminal vesicle, pancreas, muscle,...

cDNA clones representing two closely related androgen-dependent secretory proteins of 18.5 kDa were selected by screening a rat epididymal library constructed in lambda gt 11 with affinity-purified antibody directed against the 18.5-kDa proteins. The entire amino acid sequence and putative signal 18 acids was derived from 682 base pairs nucleotide overlapping clones. Confirmation identity obtained matching partial for N terminus pure protein that code cDNA. Evidence is presented difference...

1. Procedures were developed for the extraction and assay of glycolytic enzymes from epididymis epididymal spermatozoa rat. 2. The was separated into four segments analysis. When rendered free by efferent duct ligation, regional differences in enzyme activity apparent. Phosphofructokinase, glycerol phosphate dehydrogenase glucose 6-phosphate more active proximal regions epididymis, whereas hexokinase, lactate phosphorylase distal segment. These less castrated animals difference apparent...

A considerable body of information has now been assembled with regard to the major androgen-dependent secretory proteins rat epididymis. The have purified, specific antibodies developed against them, and their primary structure determined from cDNA clones. antibody probes have, in turn, used study androgenic regulation synthesis secretion proteins, distribution various tissues animal species, association sperm surface during epididymal maturation. Despite this intensive effort, precise...

The influence of androgens on the male accessory glands rat was assessed in terms changes weight and specific activity mitochondrial enzymes, succinate dehydrogenase, glycerolphosphate dehydrogenase pyruvate carboxylase, epididymis. In some instances, cytoplasmic hexokinase phosphofructokinase, also measured these enzymes found to be similar that enzymes. After administration androgen castrated rats reached a new steady state sooner than did epididymal weight. time taken for reach after...

The testes of mammals which have a scrotum are maintained at temperature several degrees below that the abdomen; mechanisms by this differential is recently been reviewed Waites (1970). In contrast to large amount information concerning testicular and its control, there no reported measurements temperatures in epididymis. present work was carried out order measure epididymal compare control organ with testis. Thermocouples were made joining iron constantin wires (36 cm × 0·16 mm) end end,...

Complementary DNA clones coding for rat androgen-binding protein (rABP) were isolated from a testis cDNA library constructed in the bacteriophage lambda gt11. The was screened immunochemically, using two different antibodies against rABP. identity of confirmed by epitope selection and sequence analysis. mRNA encoding rABP could be detected testes 20- 46-day-old-rats, but not 10-day-old rats hybridization with 32P-labeled Northern blot poly(A)+-RNA fractioned agarose gel electrophoresis. No...

Summary. The availability of glycerol in the epididymal lumen, and extent its utilization by spermatozoa, was studied measurement substrate concentration fluid oxidation vitro. source luminal sought examining penetration through epithelium a sperm-free tubule technique perfusion during intravenous infusion glycerol. [3H]Glycerol rapidly metabolized to volatile mobile molecule that quickly equilibrated between blood plasma lumen so could not be used monitor transfer. When levels were raised...

Spermatozoa from the testis and cauda epididymidis of rat were surface labelled with radioactive iodide, extracted detergent, proteins separated by two-dimensional polyacrylamide gel electrophoresis. In some instances spermatozoa also tritiated borohydride in presence galactose oxidase. Soluble blood serum, rete fluid epididymal plasma iodinated addition, aliquants sperm extracts, serum reproductive tract fluids each immunoprecipitated polyspecific antisera directed against either testicular...

Abstract Tissue pieces from the caput epididymidis of rat were incubated in vitro with ( 35 S) methionine to produce radioactive secretory proteins. The proteins so formed tested for their ability bind washed spermatozoa collected rete testis and cauda epididymidis, erythrocytes. sperm erythrocytes bound approximately 5% total protein. Binding was protein‐specific that only selected became associated cells. not cell‐specific, however, since testicular spermatozoa, caudal all same a similar degree.

1. The existing procedures for extraction of oxidized and reduced nicotinamide coenzymes were adapted to spermatozoa overcome the coenzyme-degrading activity seminal plasma. 2. content total NAD+and NADH was determined in ram, bull, boar, stallion cock. NADP+and NADPH not detected ram spermatozoa. 3. oxidation state sperm NAD depended on plasma, removal which produced a change percentage coenzyme, 100×[NAD+/(NAD++NADH)], without altering NAD++NADH. 4. In suspensions washed spermatozoa,...

Functional development of the sex accessory tissues was studied in male rat. Three potentially crucial developmental periods (neonatal, prepubertal and pubertal) were examined, then functional integrity investigated adult, when animals would have been expected to display normal function. Four (the seminal vesicles, ventral prostate caput cauda epididymides) used because their different embryological origins responses androgens adult. Synthesis secretion previously characterized...

ATM is currently considered the primary WAN technology with Internet protocols providing routing and transport requirements. Another being packet over SONET. Using Advanced Communication Technology Satellite, NASA has demonstrated experimenting these technologies. This article summarizes some of major completed ongoing experiments demonstrations performed using commercial standard such as TCP/IP broadband satellite networks.