A5050597969- Neuroendocrine regulation and behavior
- Electrolyte and hormonal disorders
- Circadian rhythm and melatonin
- Ion Transport and Channel Regulation
- Hormonal Regulation and Hypertension
- Complement system in diseases
- Sleep and Wakefulness Research
- Adenosine and Purinergic Signaling
- Stress Responses and Cortisol
- Eicosanoids and Hypertension Pharmacology
- Ion channel regulation and function
- Signaling Pathways in Disease
- Regulation of Appetite and Obesity
- Liver Disease and Transplantation
- Receptor Mechanisms and Signaling
- Neuroscience and Neuropharmacology Research
Charité - Universitätsmedizin Berlin
2016-2019
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2018-2019
Humboldt-Universität zu Berlin
2019
Freie Universität Berlin
2019
The lateral habenula (LHb) has a key role in integrating variety of neural circuits associated with reward and aversive behaviors. There is limited information about how the different cell types neuronal within LHb coordinate physiological motivational states. Here, we report type medial division (LHbM) male rats that distinguished by: (1) molecular signature for GABAergic neurotransmission (Slc32a1/VGAT) estrogen receptor (Esr1/ERα) expression, at both mRNA protein levels, as well vesicular...
Significance Statement Use of vasopressin V1a receptor (V1aR) agonists and antagonists may be instrumental for corrections acid-base homeostasis, but the distribution function this in kidney remain to characterized detail. The authors investigated whether V1aR stimulation facilitates urinary acidification via intercalated cells. Using a new specific antibody they localized basolateral membrane type A cells (A-ICs). In contrast, B exhibited intracellular not plasma membrane–bound signal. vivo...
The locus coeruleus (LC) is a brainstem nucleus distinguished by its supply of noradrenaline throughout the central nervous system. Apart from modulating range brain functions, such as arousal, cognition and stress response, LC neuronal excitability also corresponds to activity various peripheral systems, pelvic viscera cardiovascular Neurochemically diverse inputs set tone for activity, which in turn modulates these adaptive physiological behavioural responses essential survival. One...
Activation of the thick ascending limb (TAL) Na+-K+-2Cl- cotransporter (NKCC2) by antidiuretic hormone arginine vasopressin (AVP) is an essential mechanism renal urine concentration and contributes to extracellular fluid electrolyte homeostasis. AVP effects in kidney are modulated locally and/or systemically produced epoxyeicosatrienoic acid derivates (EET). The relation between EET metabolism has not been determined. Here, we show that chronic treatment AVP-deficient Brattleboro rats with...
Vasopressin (AVP) is crucial for body water and electrolyte homeostasis. Its antidiuretic effects are mediated chiefly via renal V2 vasopressin receptors (V2R). Parallel activation of V1a V1b (V1aR V1bR) may modulate the renin‐angiotensin‐aldosterone system (RAAS). Despite a large functional evidence crosstalk between AVP RAAS, little known about distribution in RAAS‐related signalling. In this study we have comparatively analyzed V1aR, V1bR, V2R kidney, adrenal glands, brain with focus on...
Vasopressin (AVP) promotes urinary concentration via its V2 receptors in the distal nephron and collecting duct of kidney. Parallel activation V1a (V1aR) has been implicated renal acid‐base handling, but cell type‐related information on this receptor type is still scarce. We have analyzed segmental cellular distribution V1aR mouse, rat, human kidneys using new antisera. Localization data were supported by functional studies. An anti‐V1aR was generated specificity verified V1aR‐knockout...
Vasopressin (AVP) drives urinary concentration via V2 receptors in the distal nephron and collecting duct of kidney. Parallel activation V1a (V1aR) has been implicated renal acid‐base handling, but cell type‐related information on this receptor is still scarce. We hypothesized that V1aR intercalated cells (IC) contributes to homeostasis by generated an anti‐V1aR antibody verified its specificity using V1aR‐knockout tissues. For localization studies mice, rat human tissue, we used...
Vasopressin (AVP) induces antidiuresis via its renal V2 receptors but also acts as a neuropeptide through activation of central V1a and V1b (V1aR, V1bR). Despite large body functional evidence for roles these in motivated behavior, little information is available on their distribution the brain tissue. This study was designed to provide comparative analysis V1aR V1bR patterns rodent human tissues using new antisera. Antibodies individual AVP were generated validated respective mouse knockout...
Calcineurin inhibitors (CnI) such as Cyclosporin A (CsA) are instrumental for immunosuppression after organ transplantation but may cause serious neurologic side effects, including seizures. Neuronal excitability depends on intact Cl − homeostasis. Generation of hyperpolarizing synaptic responses to GABA and glycine requires a gradient across the cell membrane with low [Cl ] i , mainly established by K + ‐Cl cotransporter 2 (KCC2) lesser extent Na ‐K ‐Clcotransporter 1 (NKCC1); both...
Antagonists of the V1a vasopressin receptor (V1aR) are emerging as a therapeutic strategy for retardation chronic kidney disorders. Physiologically, V1aR signaling has been linked with acid‐base homeostasis, although underlying mechanisms remain debatable. In this study, we tested hypothesis that activation in type A intercalated cells (A‐ICs) induces urinary H+ secretion. We generated an anti‐V1aR antibody and verified its specificity using V1aR‐knockout tissues. Localization studies mice,...