A5047843382- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Vibrio bacteria research studies
- Listeria monocytogenes in Food Safety
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Veterinary medicine and infectious diseases
- Bacterial Genetics and Biotechnology
- Trace Elements in Health
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Essential Oils and Antimicrobial Activity
- Probiotics and Fermented Foods
- Corrosion Behavior and Inhibition
- Pharmaceutical and Antibiotic Environmental Impacts
- Metal Extraction and Bioleaching
- Aquaculture disease management and microbiota
- Enterobacteriaceae and Cronobacter Research
- Identification and Quantification in Food
- Clostridium difficile and Clostridium perfringens research
- Legionella and Acanthamoeba research
- Arsenic contamination and mitigation
- Genomics and Phylogenetic Studies
- Porphyrin Metabolism and Disorders
University of Minnesota
2019-2025
Minnesota Department of Health
2022
University of Alberta
2012-2022
Seoul National University
2007-2019
Provincial Laboratory of Public Health
2018
China Agricultural University
2016
Iowa State University
2007-2014
University of Prince Edward Island
2011
The University of Tokyo
2003-2007
Biofilm formation of Campylobacter jejuni, a major cause human gastroenteritis, contributes to the survival this pathogenic bacterium in different environmental niches; however, molecular mechanisms for its biofilm have not been fully understood yet. In study, role oxidative stress resistance was investigated using mutants defective catalase (KatA), superoxide dismutase (SodB), and alkyl hydroperoxide reductase (AhpC). substantially increased an ahpC mutant compared wild type, katA sodB...
CosR (Campylobacter oxidative stress regulator; Cj0355c) is an OmpR-type response regulator essential for the viability of Campylobacter jejuni, a leading foodborne pathogen causing human gastroenteritis worldwide. Despite importance, function remains completely unknown mainly because cell death caused by its knockout mutation. To overcome this technical limitation, in study, antisense technology was used to investigate regulatory modulating level expression. Two-dimensional gel...
Campylobacter jejuni, a microaerophilic foodborne pathogen, inescapably faces high oxygen tension during its transmission to humans. Thus, the ability of C. jejuni survive under oxygen-rich conditions may significantly impact viability in food and safety as well. In this study, we investigated oxidative stress resistance on survival aerobic by examining three mutants defective key antioxidant genes, including ahpC, katA, sodB. All exhibited growth reduction compared wild-type (WT), ahpC...
ObjectivesTo investigate two porcine Enterococcus isolates for the genetic basis of phenicol resistance and to determine location environment novel gene.
Campylobacter jejuni is a leading food-borne pathogen, and its antibiotic resistance of serious concern to public health worldwide. C. naturally competent for DNA transformation freely takes up foreign harboring genetic information responsible resistance. In this study, we demonstrate that transfers genes more frequently in biofilms than planktonic cells by natural transformation.
The increasing resistance of Campylobacter to clinically important antibiotics, such as fluoroquinolones and macrolides, is a serious public health problem. objective this study investigate synergistic anti-Campylobacter jejuni activity macrolides in combination with phenolic compounds. Synergistic antimicrobial was measured by performing checkerboard assay ciprofloxacin erythromycin the presence 21 Membrane permeability changes C. compounds were determined measuring level intracellular...
Campylobacter is a leading foodborne pathogen worldwide. Biofilm formation an important survival mechanism that sustains the viability of under harsh stress conditions. Iron affects biofilm in some other bacteria; however, effect iron on has not been investigated Campylobacter. In this study, we discovered ferrous (Fe2+) and ferric (Fe3+) stimulated jejuni. The sequestration with chelator prevented iron-mediated stimulation. level total reactive oxygen species (ROS) biofilms was increased by...
campylobacter jejuni is a leading foodborne pathogen worldwide and its resistance to antimicrobials major concern for public health. The cmeG (Cj1375) gene in C. encodes putative efflux transporter of the facilitator family, but function antimicrobial has not been determined. This study aimed characterize CmeG conferring antibiotics oxidative stress.the was inactivated by insertional mutagenesis overexpressed cloning with shuttle vector. These constructs were compared wild-type strain using...
ABSTRACT CosR is an essential response regulator in Campylobacter jejuni , a major food-borne pathogen causing enteritis worldwide. A transcriptomic analysis performed this study discovered 93 genes whose transcriptional levels were changed >2-fold due to the repression of expression by antisense peptide nucleic acid. The identified CosR-regulated are involved various cellular functions, such as energy production, protein synthesis and folding, flagellum biogenesis, lipid metabolism....
Campylobacter jejuni is a leading cause of foodborne illnesses around the world. Since C. microaerophilic and sensitive to oxygen, aerotolerance important in transmission humans via foods under aerobic conditions. In this study, 70 strains were isolated from retail raw chicken meats subject multilocus sequence typing (MLST) analysis. testing by shaking at 200 rpm, 50 (71.4%) isolates survived after 12 h (i.e., aerotolerant), whereas 20 (28.6%) did not aerosensitive). Interestingly, further...
Human infections with Campylobacter are primarily associated the consumption of contaminated poultry meat. In this study, we isolated jejuni from retail raw chicken and duck meat in Korea compared their aerotolerance, antibiotic resistance, virulence gene prevalence. Whereas C. isolates dominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21, CC-45 is common MLST type isolates. strains both were highly tolerant aerobic stress. The prevalence genes was higher than...
Extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) are a serious public health concern worldwide. The aim of this study was to characterise ESBL-EC isolated from raw retail chicken in South Korea.The antimicrobial resistance, phylogenetic group and virulence gene prevalence 67 Korea were investigated.All the isolates possessed blaCTX-M genes, predominantly blaCTX-M-65 (52.2%) blaCTX-M-55 (25.4%), three harboured both blaCTX-M-55. More than one-half strains also carried...
Abstract Respiratory protection is key in infection prevention of airborne diseases, as highlighted by the COVID-19 pandemic for instance. Conventional technologies have several drawbacks (i.e., cross-infection risk, filtration efficiency improvements limited difficulty breathing, and no safe reusability), which yet to be addressed a single device. Here, we report development filter overcoming major technical challenges respiratory protective devices. Large-pore membranes, offering high...
Listeria monocytogenes is a foodborne pathogen exhibiting high mortality rate. In addition to the robust tolerance environmental stress, ability of L. develop biofilms increases risk contaminating food processing facilities and ultimately foods. This study aims synergistic approach better control using nisin, only bacteriocin approved as preservative, in combination with gallic-acid-rich plant extracts. Biofilm assays presence nisin gallic acid or its derivatives revealed that significantly...
Shiga toxin-producing Escherichia coli (STEC) infections pose a significant public health challenge, characterized by severe complications including hemolytic uremic syndrome (HUS) due to toxin (Stx) production. Current therapeutic approaches encounter critical limitation, as conventional antibiotic treatment is contraindicated its propensity trigger bacterial SOS response and subsequently enhance Stx production, which increases the likelihood of developing HUS in antibiotic-treated...
ABSTRACT Arsenic is commonly present in the natural environment and also used as a feed additive for animal production. Poultry major reservoir Campylobacter jejuni , food-borne human pathogen causing gastroenteritis. It has been shown that isolates from poultry are highly resistant to arsenic compounds, but molecular mechanisms responsible resistance have not determined, it unclear if acquired affects susceptibility of spp. other antimicrobials. In this study, we identified four-gene operon...
Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain and prevalent in chickens, natural reservoir for this pathogenic organism. Due importance of macrolide antibiotics clinical therapy human campylobacteriosis, development resistance has become concern public health. To facilitate control macrolide-resistant Campylobacter, it necessary understand if affects fitness transmission its host. In study we conducted pairwise competitions comingling experiments...
A number of studies from different countries have characterized mcr-1-harboring plasmids isolated food; however, nothing has been reported about it in South Korea. In this study, we report the characterization mcr-1 pan drug-resistant (PDR) Escherichia coli strains retail food country. Colistin-resistant E. were raw chicken, and PCR was carried out to detect gene. Whole genome sequencing mcr-1-positive performed for further characterization. The results whole revealed that all belonged IncI2...
Some bacteria can communicate with other species of by means autoinducer-2 (AI-2)-mediated quorum sensing. In this study, we demonstrated that AI-2-mediated sensing influences the transcription flaA, major flagellin gene in Campylobacter jejuni. A null mutation luxS C. jejuni strain 81116 reduced flaA (approximately 43% wild-type) and induced a reduction motility. However, mutant had same level total protein as wild-type. Transmission electron microscopy showed flagellar structure was...