ABSTRACT Intestinal tracts of broilers and turkeys from 10 conventional broiler farms turkey farms, where antimicrobials were routinely used, 5 organic had never been collected cultured for Campylobacter species. A total 694 isolates the poultry operations tested antimicrobial resistance to nine agents by agar dilution method. Although species highly prevalent in both operations, rates significantly different between operations. Less than 2% strains isolated organically raised resistant...

The correlation and the level of agreement between standardized agar dilution disk diffusion methods for antimicrobial susceptibility testing Campylobacter were investigated. A high-level two was evident aminoglycosides fluoroquinolones, while a low-level observed other antibiotics.

Poultry and poultry products are commonly considered as the major vehicle of Campylobacter infection in humans worldwide. To reduce number human cases, epidemiology must be better understood. Therefore, objective present study was to determine distribution genetic relatedness Thai chicken production industry. During June October 2012, entire broiler processes (i.e., breeder flock, hatchery, farm slaughterhouse) five chains were investigated chronologically. Representative isolates C. jejuni...

Campylobacter jejuni is a major zoonotic pathogen transmitted to humans via the food chain and prevalent in chickens, natural reservoir for this pathogenic organism. Due importance of macrolide antibiotics clinical therapy human campylobacteriosis, development resistance has become concern public health. To facilitate control macrolide-resistant Campylobacter, it necessary understand if affects fitness transmission its host. In study we conducted pairwise competitions comingling experiments...

Although bacterial mechanisms involved in the resistance to inorganic arsenic are well understood, molecular basis for organic has not been described. Campylobacter jejuni, a major food-borne pathogen causing gastroenteritis humans, is highly prevalent poultry and reportedly resistant compound roxarsone (4-hydroxy-3-nitrobenzenearsonic acid), which used as feed additive industry growth promotion. In this study, we report identification of novel membrane transporter (named ArsP) that...

The control of Campylobacter in poultry at the pre-harvest level is critical to reducing foodborne infections with since consumption contaminated most frequent cause human campylobacteriosis. Although vaccination suggested as useful intervention measures, no vaccines are currently available. To develop live-attenuated oral vaccines, this study, we evaluated efficacy pre-colonization by oxidative stress defense mutants, including knockout mutants ahpC, katA, and sodB, preventing jejuni from...

This study aimed to detect the cecal microbiome, antimicrobial resistance (AMR) and heavy metal genes (MRGs) in fattening pigs raised under antibiotic-free (ABF) conditions compared with ordinary industrial (control, C) using whole-genome shotgun sequencing. ABF showed enrichment of Prevotella (33%) Lactobacillus (13%), whereas Escherichia coli (40%), Fusobacterium Bacteroides (each at 4%) were notably observed C group. Distinct clusters microbiota revealed; however, some (C1) appeared same...

Presently, community-associated carbapenemase-producing Enterobacterales (CPE) remains largely unknown and require public attention. This study aimed to investigate the presence of CPE from outpatients in Thailand. Non-duplicate stool (n = 886) urine 289) samples were collected with diarrhea urinary tract infection, respectively. Demographic data characteristics patients collected. Isolation was performed by plating enrichment culture on agar supplemented meropenem. Carbapenemase genes...

Thermophilic Campylobacter, particularly Campylobacter jejuni, is one of the major foodborne human pathogens animal origin. Reliable and sensitive typing tools are required for understanding epidemiology ecology this zoonotic bacteria agent. Currently, several molecular methods available differentiating strains, but each them has limitations. Our previous study revealed that considerable sequence polymorphism exists in cmp gene encoding outer membrane protein suggested variation may be...

Tetracycline (tet) resistance in Campylobacter isolated from organically raised broilers was investigated this study. Two hundred forty-five samples an organic broiler farm were collected weekly the first week to end of production cycle, and they cultured for thermophilic Campylobacter. these isolates identified by agar dilution method, whereas DNA fingerprinting profiles tet-susceptible tet-resistant strains determined pulsed-field gel electrophoresis (PFGE). None third fourth period...

Campylobacter are bacteria associated with human foodborne disease worldwide. Poultry and poultry products generally considered as a main source of these organisms. Compared to temperate zones, baseline information on in tropical regions is limited. Thus, the objectives present study were 1) determine prevalence Thai broiler flocks 2) investigate association between climatic factors (i.e., rainfall, ambient temperature, relative humidity) colonization status Thailand. A total 442 commercial...

The dissemination of multidrug-resistant Enterobacterales (MDRE) in community settings is becoming a great concern. This study aimed to assess the incidence and risk factors associated with community-acquired urinary tract infections (CA-UTIs) caused by MDRE. A prospective case−control was undertaken among patients UTIs visiting an outpatient department Phitsanulok Province, Thailand. Urine samples were collected screened include only infections. Risk analyzed multivariate logistic...

Development of a simple, rapid and specific assay for the simultaneous detection Campylobacter spp. Salmonella based on duplex loop-mediated isothermal amplification (d-LAMP), combined with lateral-flow biosensor (LFB) is reported herein. LAMP amplicons both pathogens were simultaneously amplified specifically differentiated by LFB. The specificity d-LAMP-LFB was evaluated using set 68 target 12 non-target strains, showing 100% inclusivity exclusivity. can detect strains as low 1 ng 100 pg...

“Akahoya” is a volcanic soil classified as special deposited in Kyushu, Japan. Many of its properties are not yet clearly understood. We found that Akahoya had the potential to adsorb bacteria cattle feces, which prompted us investigate material and perform experiments comprehensively evaluate adsorption performance for various fine particles such acidic basic dyes, NOx/SOx gas, phosphoric acid ions, addition bacteria. very high specific surface area owing large number nanometer-sized pores...

The increasing incidence of antibiotic-resistant Campylobacter has become a major public health concern. Since little is known about antimicrobial resistance isolated from contracted broiler farms, the type farm that produces chicken meat for domestic consumption in Thailand, objective present study was to determine patterns and genotypes farms where antibiotics were routinely used their production. Sixty-nine jejuni isolates cloacal swabs chickens reared 2 small eastern Thailand tested...

The aim of this study was to determine the effects effective microorganisms (EM) product on ammonia reduction, growth performances, Salmonella and Campylobacter reduction hematological changes in broilers. Five hundred ninety four day-old female Arbor Acres were divided into 3 groups with 6 replicates each group. Group 1 control group, while 2 received EM forms daily spraying at 1:50 solution/m2 litter drinking water 1:800 dilution, respectively. Ammonia concentrations measured weekly, blood...

Arcobacter has been associated with foodborne illness in humans. Recently, this organism receiving more attention as a pathogen of public health concern. The contamination is frequently observed foods animal origin especially poultry products; however, the source well molecular epidemiology not clearly understood. In present study, we compared use repetitive sequence-based polymerase chain reaction (rep-PCR) and pulsed-field gel electrophoresis (PFGE) for genetic characterization Arcobacter....

ABSTRACT Akahoya is a volcanic soil rich in alumina, primarily deposited Kyushu, Japan. We have found that adsorbs bacteria the water surrounding cattle grazing areas, suggesting potential for environmental purification. This study investigated spectrum of microorganisms adsorbed by using column filled with through which suspension was passed. Shirasu soil, another different chemical composition, used as control. effectively diverse range including Escherichia coli , Campylobacter jejuni...

Spotty liver disease (SLD) affects free-range laying hens, leading to mortality and reduced egg production. Campylobacter species, including a novel strain, hepaticus, have been associated with SLD cases worldwide. However, the cause of like lesions found in broilers Japan is still remains unclear. The present study aimed investigate involvement Cam-pylobacter spp. broiler by conducting microbiological, molecular, serological, histopatho-logical, immunohistopathological examinations using...