A5102715451- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Cervical Cancer and HPV Research
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Pleural and Pulmonary Diseases
- Spinal Fractures and Fixation Techniques
- Cancer and Skin Lesions
- Esophageal Cancer Research and Treatment
- Epigenetics and DNA Methylation
- Cardiac, Anesthesia and Surgical Outcomes
- Anesthesia and Sedative Agents
- Animal Virus Infections Studies
- Colorectal and Anal Carcinomas
- Sex and Gender in Healthcare
- Cancer Diagnosis and Treatment
- Zebrafish Biomedical Research Applications
- Cancer-related gene regulation
- Obesity and Health Practices
- Trauma Management and Diagnosis
- Anesthesia and Pain Management
- Cancer, Hypoxia, and Metabolism
Rajendra Institute of Medical Sciences
2024
The University of Texas MD Anderson Cancer Center
2023
Indian Institute of Technology Kanpur
2018-2021
University of California, Irvine
2014
Kagoshima University
2007-2009
In head and neck squamous cell carcinoma (HNSCC), HRAS mutation is a new actionable oncogene driver. We aimed to evaluate mutational variants, comutation profile, survival outcomes of this molecularly defined population.We leveraged four deidentified patient data sets with HRAS-mutant HNSCC, MD Anderson Cancer Center, Kura Oncology, Inc trial, Foundation Medicine, American Association for Research GENIE v.12. Patient demographic information clinical courses were extracted, when available, in...
During recombinant Adeno-associated virus (AAV) production, a proportionately large amount of vectors is released in the culture supernatant, which often discarded. It has been shown that these associate with vesiculated structures, such as exosomes. Exosome-associated AAV (vexosomes) represent an additional gene-delivery platform. The efficiency vexosomes suicide gene therapy unexplored. In present study, we have generated serotype 6 containing inducible caspase 9 (iCasp9) by differential...
Current treatment approaches for hepatocellular carcinoma (HCC) have a narrow therapeutic index and alternate modes of are thus required. We utilized gene delivery vector containing inducible caspase 9 (iCasp9) gene, which is synthetic analogue based on the mammalian fused to human FK506 binding protein that allows its conditional dimerization synthetic, small molecule [chemical inducer dimerization, AP20187] results in target cell apoptosis. In our studies, we tested these vectors an...
Current chemotherapeutic regimens for acute myeloid leukemia (AML) have been modestly effective in patients and are associated with poor long-term survival (<30% at 5 years). Viral vector-based suicide gene therapy is an attractive option, if these vectors can target the AML cells high specificity efficiency. In this study, we developed a receptor-specific adeno-associated virus (AAV) based vector to CD33 antigen which overexpressed leukemic cells. A targeting peptide was rationally designed...
Recent success in clinical trials with recombinant Adeno-associated virus (AAV)-based gene therapy has redirected efforts optimizing AAV assembly and production, to improve its potency. We reasoned that inclusion of a small RNA during vector assembly, which specifically alters the phosphorylation status packaging cells may be beneficial. thus employed microRNAs (miR-431, miR-636) identified by their ability bind genome also dysregulate Mitogen-activated protein kinase (MAPK) signaling global...
IntroductIonThe use of regional blocks [regional anesthesia (RA)] in contemporary anesthetic practice has grown significantly.RA is the recommended approach for upper limb surgeries on cooperative patients. 1,2 For surgery, brachial plexus block (BPB) provides dependable and flexible RA. 3 The procedure most frequently performed world supraclavicular (SBPB), which less likely to cause complications since it can be done real time with ultrasound (USG)-guided SBPB.BPB employs a range...
Several independent studies have presented evidence for the involvement of human papillomaviruses (HPV) in aetiology breast cancer, while others reported opposite findings. Here, we analysed by a high sensitive multiplex PCR-based method prevalence alpha mucosal and beta cutaneous HPV DNA 90 ductal lavages, colostrum milk. Ten 70 DLs analyzed (14%) contained single or multiple types, from high-risk types was detected only one sample (1/70). A strong reduction positivity DL fluids observed 45...
Background and Purpose: Toxoplasmosis is caused by Toxoplasma gondii mainly diagnosed through serological methods, but the sensitivity insufficient. When it fails, one must rely on direct detection of parasites or DNA testing via polymerase chain reaction (PCR). Our goal to establish a molecular tool for domestic toxoplasmosis research PCR technology targeting B1 gene compare with ELISA results. Design Setting: This study was conducted at Rawal Institute Health Sciences, Islamabad between...