A5016828480- Oral microbiology and periodontitis research
- Streptococcal Infections and Treatments
- Probiotics and Fermented Foods
- Salivary Gland Disorders and Functions
- Dental Health and Care Utilization
- Oral Health Pathology and Treatment
- Bee Products Chemical Analysis
- Gut microbiota and health
- Healthcare and Venom Research
- Microbial Community Ecology and Physiology
- Immune Response and Inflammation
- Bone and Dental Protein Studies
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Bone health and treatments
- Oral health in cancer treatment
- Moringa oleifera research and applications
- Diabetes and associated disorders
- Endodontics and Root Canal Treatments
- Legionella and Acanthamoeba research
- Bone Metabolism and Diseases
- Epilepsy research and treatment
- Antimicrobial Peptides and Activities
- Dental Radiography and Imaging
- Phytochemistry and Biological Activities
Universidade de São Paulo
2011-2024
Universidade Brasil
2019
Institute of Biomedical Science
2014
Prevencia
2014
Coordenação de Aperfeicoamento de Pessoal de Nível Superior
2014
Fundação de Amparo à Pesquisa do Estado de São Paulo
2014
Although previous studies suggested an anti-inflammatory property of Brazilian red propolis (BRP), the mechanisms involved in effects BRP and its activity on macrophages were still not elucidated. This study aimed to evaluate whether attenuates inflammatory effect LPS investigate underlying mechanisms. was added RAW 264.7 murine after activation with LPS. NO production, cell viability, cytokines profile evaluated. Activation signaling pathways macrophage polarization determined by RT-qPCR...
Abstract Periodontitis is characterized by a dysbiotic microbial community and treatment strategies include the reestablishment of symbiosis reducing pathogens abundance. Aggregatibacter actinomycetemcomitans (Aa) frequently associated with rapidly progressing periodontitis. Since oral ecosystem may be affected metabolic end‐products bacteria, we evaluated effect soluble compounds released probiotic lactobacilli, known as postbiotics, on Aa biofilm expression virulence‐associated genes....
Background and Objective The potential of probiotics on the prevention control periodontitis other chronic inflammatory conditions has been suggested. Lactobacillus Bifidobacterium species influence P. gingivalis interaction with gingival epithelial cells (GECs) but may not act in a unique way. In order to select most appropriate probiotic against gingivalis, we aimed evaluate effect several strains Porphyromonas biofilm formation transcription virulence-associated factors (PgVAFs).Methods...
Inflammation is a driven force in modulating microbial communities, but little known about the interplay between colonizing microorganisms and immune response periodontitis. Since local systemic inflammation may play whole role disease, we aimed to evaluate oral fecal microbiome of patients with periodontitis correlate data levels inflammatory mediator saliva. Methods: Nine (P) Stage 3/Grade B nine age-matched non-affected controls (H) were evaluated. Microbial communities biofilms (the...
Objective This study aimed to evaluate the effect of antimicrobial photodynamic therapy (aPDT) and use probiotics on treatment halitosis. Methods Fifty-two participants, aged from 18 25 years, exhaling sulfhydride (H 2 S) ≥ 112 ppb were selected. They allocated into 4 groups (n = 13): Group 1: tongue scraper; 2: treated once with aPDT; 3: probiotic capsule containing Lactobacillus salivarius WB21 (6.7 x 10 8 CFU) xylitol (280mg), 3 times a day after meals, for 14 days; 4: aPDT days....
Diabetes has been associated with periodontitis, but the mechanisms through which periodontal diseases affect metabolic control remain unclear. Objective: This study aimed to evaluate serum leveis of inflammatory markers, IL-8, IL-6 and monocyte chemoattractant protein 1 (MCP-1), in type 2 diabetic patients presence chronic periodontitis. Material Methods: Forty two individuals were enrolled this assigned one five groups: diabetes mellitus inadequate glycemic periodontitis (DMI+P, n = 10),...
In order to improve our understanding on the microbial complexity associated with Grade C/molar-incisor pattern periodontitis (GC/MIP), we surveyed oral and fecal microbiomes of GC/MIP compared non-affected individuals (Control). Seven Afro-descendants seven age/race/gender-matched controls were evaluated. Biofilms from supra/subgingival sites (OB) feces collected submitted 16S rRNA sequencing. Aggregatibacter actinomycetemcomitans (Aa) JP2 clone genotyping salivary nitrite levels...
Introduction: Very little is known of the diversity and expression virulence factors serotypes Aggregatibacter actinomycetemcomitans . Toxic activity on Chinese hamster ovary (CHO) cells cdt ltx genotyping were evaluated in A. serotypes. Methods: Forty‐one isolates analysed for CHO cell growth inhibition. Genotyping was performed by polymerase chain reactions specific to promoter region, serotype‐specific region sequencing cdtB Results: cdtABC detected 40 strains. Analysis cdtA upstream...
Probiotics may be considered as an additional strategy to achieve a balanced microbiome in periodontitis. However, the mechanisms underlying use of probiotics prevention or control periodontitis are still not fully elucidated. This vitro study aimed evaluate effect two commercially available strains lactobacilli on gingival epithelial cells (GECs) challenged by Aggregatibacter actinomycetemcomitans . OBA-9 GECs were infected with A. strain JP2 at MOI 1:100 and/or co-infected Lactobacillus...
Pinheiro ET, Kawamoto D, Ota-Tsuzuki C, Almeida LRS, Nunes ACR, Longo PL, Wikstrom M, Mayer MPA. Analysis of genotypic variation in genes associated with virulence Aggregatibacter actinomycetemcomitans clinical isolates. J Periodont Res 2011; 46: 310–317. © 2011 John Wiley & Sons A/S Background and Objective: Although certain serotypes are more aggressive periodontitis than other serotypes, the correlation between distinct lineages traits this species is poorly understood. This study aimed...
The dysbiotic microbiota associated with aggressive periodontitis includes Aggregatibacter actinomycetemcomitans, the only oral species known to produce a cytolethal distending toxin (AaCDT). Give that CDT alters cytokine profile in monocytic cells, we aimed test hypothesis plays role bone homeostasis by affecting differentiation of precursor cells into osteoclasts. Recombinant AaCDT was added murine marrow monocytes (BMMC) presence or absence RANKL and cell viability osteoclast were...
Background Cerebral palsy ( CP ) individuals present with epilepsy, which requires the use of antiepileptic drug AED ). Hypothesis Since an inflammatory response may contribute to epileptogenesis, hypothesis tested was that constipation would be associated gingivitis and in children adolescents CA . Design A comparative study conducted 101 aged 5‐17 years (10.8 ± 4.9), classified as constipated (G1; n = 57) or not (G2; 44). Clinical patterns, used, body mass index BMI ), fluid intake, toilet...
The benefits of probiotics on dysbiotic microbiomes and inflammation are dependent the tested strain, host factors, resident microbiome. There is limited knowledge effects in A. actinomycetemcomitans-associated periodontitis. Thus, Lactobacillus acidophilus LA5 (LA5) was orally inoculated for 30 days C57Bl/6 mice infected with actinomycetemcomitans JP2 (Aa) S. gordonii (Sg). Alveolar bone loss, gingival gene expression, oral gut were determined. controlled loss Aa+Sg-infected mice,...
Periodontitis is a destructive inflammatory response triggered by dysbiosis. Lactobacillus acidophilus LA5 (LA5) may impair microbial colonization and alter the host. Thus, we evaluated effect of on alveolar bone loss in periodontitis murine model investigated its oral gut microbiomes. Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Streptococcus gordonii were inoculated C57BL/6 mice (P+), with (L+). SHAM infected controls (P- and/or L- groups) also evaluated. After...
Periodontitis is an inflammatory disease induced by a dysbiotic oral microbiome. Probiotics of the genus Bifidobacterium may restore symbiotic microbiome and modulate immune response, leading to periodontitis control. We evaluated effect two strains able inhibit Porphyromonas gingivalis interaction with host cells biofilm formation, but distinct immunomodulatory properties, in mice model. Experimental (P+) was C57Bl/6 microbial consortium human organisms. B. bifidum 162 2A [B+ (1622)] breve...
The isoflavone (3S)-vestitol, obtained from red propolis, has exhibited anti-inflammatory, antimicrobial, and anti-caries activity; however, few manuscripts deal with its anti-inflammatory mechanisms in macrophages. objective is to elucidate the of (3S)-vestitol on those cells. Peritoneal macrophages C57BL6 mice, stimulated lipopolysaccharide, were treated 0.37 0.59 µM for 48 h. Then, nitric oxide (NO) quantities, viability, release 20 cytokines transcription several genes related cytokine...
Oral bacteria are implicated not only in oral diseases but also gut dysbiosis and inflammatory conditions throughout the body. The periodontal pathogen
Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in pathogenesis periodontitis. P. has an arsenal specialized virulence factors that contribute to its pathogenicity. Among them, fimbriae play a role initial attachment and organization biofilms. Different genotypes fimA have been related length pathogenicity bacterium. Objectives The aim this study was identify 5 types genotype strains smokers nonsmokers with periodontitis, before after...
Aggregatibacter actinomycetemcomitans (Aa) is abundant within the microbial dysbiotic community of some patients with periodontitis. Aa outer membrane protein 29 (OMP29), a member OMPA family, mediates invasion to gingival epithelial cells (GECs). This study evaluated effect OMP29 and its paralogue OMP29par on response GECs Aa. The omp29 or/and omp29par deletion mutants AaΔ29, AaΔ29P, AaΔ29Δ29P were constructed, recombinant OMP29His was obtained. Microarray analysis evaluation cxcl-8 gene...
This study evaluated systemic bacteremia caused by chewing in a 42-year-old male with chronic severe periodontitis and uncontrolled type 2 diabetes mellitus. Aerobic anaerobic Gram-positive bacilli were detected culture, Aggregatibacter actinomycetemcomitans Porphyromonas gingivalis real time polymerase chain reaction blood samples.