A5055603134- RNA Research and Splicing
- Stochastic processes and financial applications
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Insurance, Mortality, Demography, Risk Management
- CRISPR and Genetic Engineering
- Coenzyme Q10 studies and effects
- Adipose Tissue and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Financial Risk and Volatility Modeling
- Risk and Portfolio Optimization
ETH Zurich
2001-2023
RNA helicases of the DEAH/RHA family are involved in many essential cellular processes, such as splicing or ribosome biogenesis, where they remodel large RNA-protein complexes to facilitate transitions next intermediate. DEAH couple adenosine triphosphate (ATP) hydrolysis conformational changes their catalytic core. This movement results translocation along RNA, which is held place by auxiliary C-terminal domains. The activity proteins strongly enhanced and diverse class G-patch activators....
SUMMARY The spliceosome is a staggeringly complex machine comprising, in humans, 5 snRNAs and >150 proteins. We scaled haploid CRISPR-Cas9 base editing to target the entire human interrogated mutants using U2 snRNP/SF3b inhibitor, pladienolide B. Hypersensitive substitutions define functional sites U1/U2-containing A-complex but also components that act as late second chemical step after SF3b dissociated. Viable resistance map not only B binding site G-patch (ATPase activator) domain of...