Previously, we reported gold nanoparticles coated with semifluorinated ligands self-assembled into nanoparticle vesicles (AuNVs) a sub-100 nm diameter in tetrahydrofuran (THF). (1) Although this size is potentially useful for vivo use, the biomedical applications of AuNVs were limited, as vesicular structure collapsed water. In paper, demonstrate that can be dispersed water by cross-linking each thiol-terminated PEG so cross-linked work drug delivery carrier enabling light-triggered release....

Gold nanoparticles (GNPs) show promise as both drug and imaging carriers with applications in diagnosis therapy. For the safe effective use of such gold nanomaterials biomedical field, it is crucial to understand how size shape affect their biological features, vitro cellular uptake speed accumulation well cytotoxicity. Herein, we focus on triangular (TNPs) four different sizes (side length 46, 55, 72, 94 nm; thickness 30 nm) compare internalization efficiency those spherical (SNPs) various...

We developed a substrate that enables highly sensitive and spatially uniform surface-enhanced Raman scattering (SERS). This comprises densely packed gold nanoparticles (d-AuNPs)/titanium dioxide/Au film (d-ATA). The d-ATA demonstrates modal ultrastrong coupling between localized surface plasmon resonances (LSPRs) of AuNPs Fabry–Pérot nanocavities. exhibits significant enhancement the near-field intensity, resulting in 78-fold increase SERS signal for crystal violet (CV) compared to...

Surface-enhanced Raman scattering (SERS) is an attractive technique in molecular detection with high sensitivity and label-free characteristics. However, its use protein limited by the large volume of proteins, hindering approach to narrow spaces hotspots. In this study, we fabricated a Au nanoTriangle plate Array on Gel (AuTAG) as SERS substrate attaching (AuNT) arrangement thermoresponsive hydrogel surface. The AuTAG acts actively tunable plasmonic device, which interparticle distance...

Overexpression of the ATP-binding cassette transporter ABCG2 reportedly causes multidrug resistance, whereas altered drug-resistance profiles and substrate specificity are implicated for certain variant forms ABCG2. At least three have been hitherto documented on basis their amino acid moieties (i.e., arginine, glycine threonine) at position 482. In present study we generated those variants by site-directed mutagenesis expressed them in HEK-293 cells. Exogenous expression Arg(482), Gly(482),...

We report the layer-by-layer (LbL) preparation of multilayered thin films that consist solely DNA. The properties were varied by assembling layers from different oligonucleotide building blocks, which are composed repeating homopolymeric units nucleotides [adenosine (A), cytosine (C), guanine (G), and thymidine (T)] or "random" sequences. Films assembled oligonucleotides with a single complementary unit did not show continual layer buildup. To form multilayer system, it was necessary for...

Active gap control on surface-enhanced Raman scattering (SERS) measurements is performed using tunable plasmonic nanostructures, which are fabricated through the formation of gold nanoparticle self-assembled thin films solid substrates and their subsequent transference onto polyacrylic acid gel. When hemoproteins used as targets, signal intensities increased more than 10-fold due to this active control. As a service our authors readers, journal provides supporting information supplied by...

Abstract Sophisticated control of the spatial arrangement gold nanorods provides significant advantages in design plasmonic systems. However, dynamic modulation nanorod arrangements remains challenging. Here, we present a novel strategy for thermo-responsive with uniform alignment on solid substrate using polymer brushes. In this system, cationic and were immobilized into anionic brushes via moderate electrostatic interactions, providing vertically aligned arrays. Upon heating, assembled...

Cartilage is a connective tissue composed of mainly water, collagen (COL) and proteoglycans (PGs) including chondroitin sulfate (CS). Near-infrared (NIR) spectroscopy adequate for examination soft hard tissues with large amount water non-destructively non-invasively. We measured tablets containing CS COL using NIR to develop an evaluation method PGs in cartilage Calibration curves were constructed the spectra that show quantitative linear relationship between concentration height second...

Abstract Delivery of exogenous nucleic acids (NAs) for gene regulation in bacteria, bypassing the barrier cell wall, is essential advancing fundamental microbiology and genetic engineering, treatment bacterial diseases. However, current methods that rely on electrical or chemical interventions are limited by their complexity, specialized expertise, laboratory‐specific instrumentation. This study explores capability gold nanoclusters (AuNCs) as carriers delivering small‐interfering RNA...

This study aims at the synthesis of Janus gold nanoparticles (Janus GNPs) with hydrophilic/hydrophobic faces by a simple ligand exchange reaction in an homogeneous system and elucidation self-assembled structures GNPs water. As hydrophilic surface ligands, we synthesized hexaethylene glycol (E6)-terminated thiolate ligands C3, C7, or C11 alkyl chains, referred to as E6C3, E6C7, E6C11, respectively. hydrophobic ligand, butyl-headed C4-E6C11, which C4 was introduced on E6C11 terminus,...

We demonstrate that binary mixtures of small and large gold nanoparticles (GNPs) (5/15, 5/30, 10/30, 15/30 nm in diameter) the presence a glucose-terminated fluorinated oligo(ethylene glycol) ligand can spontaneously form size-segregated assemblies. The outermost layer assembly is composed single small-sized GNPs, while larger-sized GNPs are located interior, forming what referred to as yolk/shell assembly. Time course study reveals aggregate together, these kinetically trapped aggregations...

The revolutionary idea that DNA can play a non-biological role in material world has indeed become today's reality. Although much attentions have recently been paid to "structural nanotechnology" centered on origami, due the need for computation, hybridization and modification are usually not easily accessed, reproduced, or manipulated, preparation of nanostructures using origami may yet set threshold researcher advance their fields with functional nanomaterials. It is suggested that,...

Stimuli-responsive assembly of gold nanoparticles (AuNPs) with precise control the plasmonic properties, size, and stimuli responsivity has shown potential benefits regard to biosensing devices drug-delivery systems. Here we present a new pH-responsive coassembly system oligo(ethylene glycol) (OEG)-coated AuNPs anionic polymers as an external mediator via hydrogen bonding in water. Hydrogen-bond-driven coassemblies OEG-AuNPs poly(acrylic acid) (PAA) were confirmed by monitoring peaks...

Abstract Gold nanorods (GNRs) coated with a single kind of ligand show thermoreponsive two‐step assembly to provide hierarchical structure. The GNRs (33 nm in length × 14 diameter) hexa(ethylene glycol) (HEG) derivative form side‐by‐side assemblies at 30 °C ( T A1 ) as steady state through dehydration. By further heating over 40 A2 ), larger assemblies, which are composed the assembled units, formed structures. dehydration temperature HEG varies depending on free volume unit, corresponds...

Conjugation with gold nanorods enhanced the adjuvanticity of RNA adjuvant for intranasal inactivated influenza vaccines, providing efficient protection against infection in mice.

Abstract Gold nanorods (AuNRs) have unique optical properties such as transverse and longitudinal localized surface plasmon resonance (T‐ L‐LSPR). As the L‐LSPR absorption depends on angle of AuNRs to incident light polarization, orientational control is a crucial issue. In spite various techniques AuNR orientation, dynamic orientation tuning solid substrate remains challenging. Herein, changes are demonstrated in anionic polymer (DNA) brushes via their thickness by salt concentration....

The mixing behavior of an exchangeable phospholipid (A) with sterol (B) in host bilayers made from 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) containing varying concentrations cholesterol has been examined via the nearest-neighbor recognition method. At low (i.e., 2.5 mol %), between A and B is close to ideal. Incremental increases concentration 40 % led net affinity B. Similar experiments that were carried out presence chloroform showed a leveling effect, where moderate...

This paper presents the thermoresponsive assembly behaviors of gold nanoparticles (AuNPs; 3, 5, and 10 nm in diameter) that are coated with a self-assembled monolayer oligo(ethylene glycol) (OEG) ligands terminated alkyl heads. AuNPs (5 OEG without an head did not assemble within temperature range from 20 to 70 °C. However, ethyl, iso-propyl, propyl-headed afforded at temperatures 56, 33, 19 °C, respectively, indicating can be tuned over wide by slight changes hydrophobicity head. Almost no...

The development of a strategy for the assembly nanoscale building blocks, in particular, anisotropic nanoparticles, into desired structures is important construction functional materials and devices. However, control over orientation rod-shaped nanoparticles on substrate integration solid-state devices remains challenging. Here, we report fabrication finely aligned gold nanorod (GNR) arrays using polymer (DNA) brushes as template. nanorods modified with cationic surface ligands were...

Pattern fabrication was performed by transcribing Au nano-dots from Si to the hydrogel surface. The exhibited excellent adhesion despite extensive expansion and contraction. on a can produce iridescent color, its spectral response be finely modulated controlling nano-dot spacing. This is novel tunable photonic crystal made soft wet matter.

Silver halide (AgX, X = Cl, Br, I)-based materials represent an emerging class of heterogeneous photocatalysts. Despite progress in the synthesis carrier-separated AgX-based photocatalysts, a number issues remain unaddressed, including complicated synthesis, unfavorably large size and therefore poor photocatalytic performance resultant structures. Here we show one-step DNA-programmable Ag/AgCl nanostructures that takes only approximately 1 min for application. The optimal DNA-encapsulated...

The nanoparticle-based delivery of siRNA with a noncationic outermost surface at low particle concentration is greatly desired. We newly synthesized bifurcated ligand (BL) possessing hydrophobic and hydrophilic arms as for gold nanoparticles (AuNPs) to allow delivery. concept underlying the design this that amphiphilic property should AuNPs permeate cell cytosol thorough endosomal membrane. BLs quaternary cationic ligands were codisplayed on 40 nm AuNPs, which subsequently coated via...