A5087681080- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- Protein purification and stability
- Crystallography and molecular interactions
- Protein Structure and Dynamics
- Atomic and Molecular Physics
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Microbial Natural Products and Biosynthesis
- Enzyme Production and Characterization
- Biochemical and Molecular Research
- Metalloenzymes and iron-sulfur proteins
- Porphyrin Metabolism and Disorders
- Laser-Matter Interactions and Applications
- Metal complexes synthesis and properties
- Magnetism in coordination complexes
- Carbohydrate Chemistry and Synthesis
- Photosynthetic Processes and Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Metal-Catalyzed Oxygenation Mechanisms
- Polysaccharides and Plant Cell Walls
- Chemical Synthesis and Analysis
- Crystal structures of chemical compounds
University of Copenhagen
2003-2025
Technical University of Denmark
2014-2023
Ørsted (Denmark)
1986-2022
Novosibirsk State University
2009
Nikolaev Institute of Inorganic Chemistry
2009
Universitat Jaume I
2009
Roskilde University
2002-2004
European Synchrotron Radiation Facility
2004
Utrecht University
2002
Centre National de la Recherche Scientifique
2000
Non-natural peptide analogs have significant potential for the development of new materials and pharmacologically active ligands. One such architecture, β-peptoids (N-alkyl-β-alanines), has found use in a variety biologically compounds but been sparsely studied with respect to folding propensity. Thus, we here report an investigation effect structural variations on cis-trans amide bond rotamer equilibria selection monomer model systems. In addition various side chain effects, which...
This first structure of the enzyme converting dopamine to norepinephrine provides new perspectives on numerous disorders.
Soil microbiota can confer fitness advantages to plants and increase crop resilience drought other abiotic stressors. However, there is little evidence on the mechanisms correlating a microbial trait with plant stress tolerance. Here, we report that Streptomyces effectively alleviate salinity by producing spiroketal polyketide pteridic acid H (1) its isomer F (2), both of which promote root growth in Arabidopsis at concentration 1.3 nM under stress. Transcriptomics profiles show increased...
Abstract β-Peptoids are peptidomimetics based on N -alkylated β-aminopropionic acid residues (or -alkyl-β-alanines). This type of peptide mimic has previously been incorporated in biologically active ligands and hypothesized to be able exhibit foldamer properties. Here we show, for the first time, that β-peptoids can tuned fold into stable helical structures. We provide high-resolution X-ray crystal structures homomeric β-peptoid hexamers, which reveal right-handed conformations with exactly...
The mechanism for the iridium-BINAP catalyzed dehydrogenative decarbonylation of primary alcohols with liberation molecular hydrogen and carbon monoxide was studied experimentally computationally. reaction takes place by tandem catalysis through two catalytic cycles involving dehydrogenation alcohol resulting aldehyde. square planar complex IrCl(CO)(rac-BINAP) isolated from between [Ir(cod)Cl]2, rac-BINAP, benzyl alcohol. catalytically active applied in study individual steps cycles. One...
Orotidine 5'-monophosphate decarboxylase (ODCase) catalyzes the decarboxylation of orotidine 5'-monophosphate, last step in de novo synthesis uridine 5'-monophosphate. ODCase is a very proficient enzyme [Radzicka, A., and Wolfenden, R. (1995) Science 267, 90−93], enhancing reaction rate by factor 1017. This proficiency has been enigmatic, since it achieved without metal ions or cofactors. Here we present 2.5 Å resolution structure complexed with inhibitor...
We have observed nonresonant multiphoton electron detachment of ${\mathrm{H}}^{\mathrm{\ensuremath{-}}}$ ions in moderately intense (a few tens GW/${\mathrm{cm}}^{2}$) laser fields. A well-collimated beam with an energy 581 MeV was intersected by focused 10.6-\ensuremath{\mu}m radiation from a pulsed ${\mathrm{CO}}_{2}$ laser. The center-of-mass photon tuned using the relativistic Doppler shift so that minimum number simultaneous photons required for ranged three to sixteen. Definite signals...
Several series of doubly excited resonances have been observed in ${\mathrm{H}}^{\mathrm{\ensuremath{-}}}$ photodetachment, appearing as dips the production cross section neutral hydrogen. At least three are clearly visible converging on each thresholds n=5, 6, 7, and 8. The data support recent theoretical calculations for energies verify a Rydberg-dipole formula main resonance positions.
Therapeutic protein candidates should exhibit favorable properties that render them suitable to become drugs. Nevertheless, there are no well-established guidelines for the efficient selection of proteinaceous molecules with desired features during early stage development. Such can emerge only from a large body published research employs orthogonal techniques characterize therapeutic proteins in different formulations. In this work, we share study on diverse group proteins, including their...
Abstract Oculocutaneous albinism (OCA) is a genetically heterogeneous disorder. Six genes are associated with autosomal recessive OCA ( TYR , OCA2 TYRP1 SLC45A2 SLC24A5 and LRMDA ), one gene, GPR143 X-linked ocular (OA). Molecular genetic analysis provides diagnosis in approximately 60% of individuals clinical OA/OCA. A considerably number the remaining 40% heterozygous for causative sequence variation . To identify missing variants these, we used NGS based approach, genotyping segregation...
The sirtuins are NAD+ -dependent lysine deacylases, comprising seven isoforms (SIRT1-7) in humans, which involved the regulation of a plethora biological processes, including gene expression and metabolism. share common hydrolytic mechanism but display preferences for different ϵ-N-acyllysine substrates. SIRT7 deacetylates targets nuclei nucleoli remains one lesser studied isoforms, part due to lack chemical tools specifically probe activity. Here we expressed and, using small-angle X-ray...
Sine Larsen, former President and General Secretary Treasurer of the IUCr, is remembered.
Laser photodetachment on a relativistic ${\mathrm{H}}^{\mathrm{\ensuremath{-}}}$ beam has revealed several series of resonances in which both electrons are excited. These ``bound states the continuum'' appear as dips partial production cross sections various excited neutral hydrogen. We present here detailed account experimental observation and results fits measured yields to sets Fano profiles. A simple analytic formula for energy-level spacing this quantum-mechanical three-body system is verified.
Due to low ion permeability of lipid bilayers, it has been and still is common practice use transporter molecules such as ionophores or lipophilic chelators increase transmembrane diffusion rates loading efficiencies radionuclides into liposomes. Here, we report a novel very simple method for the positron emitter 64Cu2+ liposomes, which important in vivo emission tomography (PET) imaging. By this approach, copper added liposomes entrapping chelator, causes spontaneous across bilayer where...
Multiphoton detachment from ${\mathrm{H}}^{\mathrm{\ensuremath{-}}}$ has been studied using linearly polarized light for photon energies 0.15 to 0.39 eV. Characteristic threshold structures were observed. The electron rates are estimated at peak laser intensities 2 12 GW/${\mathrm{cm}}^{2}$ in the laboratory frame. two-photon data reveal intensity-dependent shifts of energy.
A study is presented of the photodetachment cross section ${\mathrm{H}}^{\mathrm{\ensuremath{-}}}$ ion near one-electron threshold in electric fields ranging from approximately 5\ifmmode\times\else\texttimes\fi{}${10}^{\mathrm{\ensuremath{-}}7}$ up to 2.4\ifmmode\times\else\texttimes\fi{}${10}^{\mathrm{\ensuremath{-}}4}$ a.u. The lowest-field data, nominally at zero field, are consistent with Wigner law for p-wave processes. At larger field values, using \ensuremath{\sigma}-polarized laser...
Tryptophan hydroxylase (TPH) is a mononuclear non-heme iron enzyme, which catalyzes the reaction between tryptophan, O2, and tetrahydrobiopterin (BH4) to produce 5-hydroxytryptophan 4a-hydroxytetrahydrobiopterin. This first rate-limiting step in biosynthesis of neurotransmitter hormone serotonin (5-hydroxytryptamine). We have determined 1.9 Å resolution crystal structure catalytic domain (Δ1−100/Δ415−445) chicken TPH isoform 1 (TPH1) complex with tryptophan substrate an iron-bound imidazole....
Cyclic tetrapeptide and depsipeptide natural products have proven useful as biological probes drug candidates due to their potent activities histone deacetylase (HDAC) inhibitors. Here, we present the syntheses of a class cyclic HDAC inhibitors, azumamides, by concise route in which key step preparation noncanonical disubstituted β-amino acid building block was an Ellman-type Mannich reaction. By tweaking reaction conditions during this transformation, gained access well two epimeric...
In the present study, small-angle X-ray scattering (SAXS) and static light (SLS) have been used to study solution properties self-interaction of recombinant human serum albumin (rHSA) molecules in three pharmaceutically relevant buffer systems. Measurements are carried out up high protein concentrations as a function ionic strength by adding sodium chloride probe role electrostatic interactions. The effective structure factors (Seff) vector magnitude q extracted from profiles fit...
Rhamnogalacturonan lyase (RG‐lyase) specifically recognizes and cleaves α‐1,4 glycosidic bonds between l ‐rhamnose d ‐galacturonic acids in the backbone of rhamnogalacturonan‐I, a major component plant cell wall polysaccharide, pectin. The three‐dimensional structure RG‐lyase from Aspergillus aculeatus has been determined to 1.5 Å resolution representing first known polysaccharide family 4 an enzyme with this catalytic specificity. 508‐amino acid polypeptide displays unique arrangement three...