A5034779169- Diabetes Treatment and Management
- Regulation of Appetite and Obesity
- Pancreatic function and diabetes
- Biochemical Analysis and Sensing Techniques
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Receptor Mechanisms and Signaling
- Helicobacter pylori-related gastroenterology studies
- Ion Transport and Channel Regulation
- Bariatric Surgery and Outcomes
- Renal function and acid-base balance
- Gastrointestinal motility and disorders
- Neuropeptides and Animal Physiology
- Digestive system and related health
- Electrolyte and hormonal disorders
University of Copenhagen
2018-2025
Novo Nordisk Foundation
2019-2022
Protein intake robustly stimulates the secretion of incretin hormone, glucagon-like peptide-1 (GLP-1) but molecular mechanisms involved are not well understood. In particular, it is unknown whether proteins stimulate by activation luminal or basolateral sensors. We characterized using a physiologically relevant model - isolated perfused proximal rat small intestine. Intraluminal protein hydrolysates derived from meat (peptone; 50 mg/mL) increased GLP-1 2.3-fold (from basal 110 ± 28...
The aim of this study was to explore individual amino acid-stimulated GLP-1 responses and the underlying stimulatory mechanisms, as well identify acid-sensing receptors involved in release. Experiments were primarily based on isolated perfused rat small intestines, which have intact epithelial polarization allowing discrimination between luminal basolateral mechanisms quantitative studies intestinal absorption hormone secretion. Expression analysis acid sensors murine secreting L-cells...
Abstract Aim Intestinal glucose transport involves SGLT1 in the apical membrane of enterocytes and GLUT2 basolateral membrane. In vivo studies have shown that absorption rates appear to exceed theoretical capacity these transporters, suggesting may occur via additional pathways, which could include passive mechanisms. The aim study was investigate an vitro model, has proven useful for endocrine studies. Methods We studied both transcellular paracellular isolated vascularly perfused rat small...
Gastrointestinal hormones contribute to the beneficial effects of Roux-en-Y gastric bypass surgery (RYGB) on glycemic control. Secretin is secreted from duodenal S cells in response low luminal pH, but it unknown whether its secretion altered after RYGB and if secretin contributes postoperative improvement We hypothesized that increases as a result diversion nutrients more distal parts small intestine, thereby affects islet hormone release.A specific radioimmunoassay was developed, evaluated...
Fiber-free diet impairs intestinal and colonic health in mice, parallel with a reduction glucagon like peptide-1 (GLP-1) levels. Endogenous GLP-1 is important for growth maintenance of the integrity. We aimed to investigate whether fiber-free reduces luminal content metabolites which, upon supplementation, could increase secretion restore adverse effects diet.
Significance Statement During acute base excess, the renal collecting duct β -intercalated cells ( -ICs) become activated to increase urine excretion. This process is dependent on pendrin and cystic fibrosis transmembrane regulator (CFTR) expressed in apical membrane of -ICs. The signal that leads activation this was unknown. Plasma secretin levels during alkalosis, receptor (SCTR) functionally We find mice with global knockout for SCTR lose their ability acutely forces lower ventilation...
Abstract Background We previously reported that, among all the naturally occurring amino acids, l -valine is most powerful luminal stimulator of glucagon-like peptide 1 (GLP-1) release from upper part rat small intestine. This makes an interesting target for nutritional-based modulation GLP-1 secretion. However, molecular mechanism -valine-induced secretion remains unknown. Methods aimed to investigate effect orally given in mice and identify details stimulated using isolated perfused...
Gastrin and cholecystokinin (CCK) are hormones released from endocrine cells in the antral stomach (gastrin), duodenum, jejunum (CCK). Recent reports, based on secretion experiments an enteroendocrine cell line (NCI-H716) gastrin receptor expression proglucagon-expressing rat colon, suggested that could be a regulator of glucagon-like peptide-1 (GLP-1) secretion. To investigate these findings, we studied acute effects CCK-8 (a CCK1/CCK2 (gastrin) agonist) gastrin-17 CCK2(gastrin) robust ex...
The molecular sensors underlying nutrient-stimulated GLP-1 secretion are currently being investigated. Peripheral administration of melanocortin-4 receptor (MC4R) agonists have been reported to increase plasma concentrations in mice and humans but it is unknown whether this effect results from a direct on the secreting L-cells intestine, other effects intestine or extra-intestinal effects. We investigated L-cell expression MC4R mouse human by reanalyzing publicly available RNA sequencing...
Abstract Objective Gastrointestinal hormones contribute to the beneficial effects of Roux-en-Y gastric bypass surgery (RYGB) on glycemic control. Secretin is secreted from duodenal S-cells in response low luminal pH, but it unknown whether its secretion altered after RYGB and if secretin contributes post-operative improvement We hypothesized that increases as a result diversion nutrients more distal parts small intestine, thereby affects islet hormone release. Methods A specific...