A5055464136- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- Testicular diseases and treatments
- Glycosylation and Glycoproteins Research
- Diabetes Treatment and Management
- Pancreatic function and diabetes
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- RNA Research and Splicing
- Monoclonal and Polyclonal Antibodies Research
- Tumors and Oncological Cases
- Proteoglycans and glycosaminoglycans research
- Muscle Physiology and Disorders
- Galectins and Cancer Biology
- Connective tissue disorders research
- Pituitary Gland Disorders and Treatments
- Sarcoma Diagnosis and Treatment
- Caveolin-1 and cellular processes
- Drug Transport and Resistance Mechanisms
- Cellular Mechanics and Interactions
- Bone and Dental Protein Studies
- Growth Hormone and Insulin-like Growth Factors
University of Copenhagen
2015-2025
University of Turku
2011
University of Massachusetts Chan Medical School
2011
Kyoto University
2011
Aarhus University
2011
Temple University
2006
Sanford Burnham Prebys Medical Discovery Institute
1995
Pennsylvania State University
1995
Massachusetts General Hospital
1994
Harvard University
1994
Abstract Systems-wide profiling of breast cancer has almost always entailed RNA and DNA analysis by microarray sequencing techniques. Marked developments in proteomic technologies now enable very deep clinical samples, with high identification quantification accuracy. We analysed 40 oestrogen receptor positive (luminal), Her2 triple negative tumours reached a quantitative depth >10,000 proteins. These profiles identified functional differences between subtypes, related to energy...
Bile acids (BAs) facilitate fat absorption and may play a role in glucose metabolism regulation, stimulating the secretion of gut hormones. The relative importance mechanisms involved BA-stimulated appetite regulating hormones from pancreas is not well described was purpose this study. effects bile on pancreatic studied rats compared to most nutritional secretagogue: glucose. molecular that underlie by isolated perfused rat mouse small intestine preparations supported immunohistochemistry,...
The ADAM (A DisintegrinAnd Metalloprotease) family of cell-surface proteins may have an important role in cellular interactions and modulating responses. In this report we describe a novel, secreted form human 12 (meltrin α), designated 12-S (S for short), larger, membrane-bound 12-L (L long form). These two forms arise by alternative splicing single gene located on chromosome 10q26. Northern blotting demonstrated that mRNAs both are abundant term placenta also present some tumor cell lines....
The human laminin receptor was purified and molecularly cloned to investigate its biosynthetic regulation. Laminin from normal neoplastic tissue preparatively affinity homogeneity based on the high of for laminin. apparent molecular weight different carcinoma sources placental is in range 68-72 kDa. Isoelectric focusing two-dimensional gel electrophoresis indicated that protein consists one major polypeptide chain with a pI value 6.4 +/- 0.2. cDNA clones were isolated after screening...
The ADAMs (a disintegrin and metalloprotease) family of proteins is involved in a variety cellular interactions, including cell adhesion ecto- domain shedding. Here we show that ADAM 12 binds to surface syndecans. Three forms recombinant were used these experiments: the cys-teine-rich made Escherichia coli (rADAM 12-cys), disintegrin-like cysteine-rich insect cells 12-DC), full-length human 12-S tagged with green fluorescent protein mammalian 12-GFP). Mesenchymal specifically dose-dependent...
The ADAMs (<i>a d</i>isintegrin<i>a</i>nd <i>m</i>etalloprotease) are a family of multidomain proteins with structural homology to snake venom metalloproteases. We recently described the cloning and sequencing human <i>ADAM 12</i> (meltrin α). In this report we provide evidence that metalloprotease domain ADAM 12 is catalytically active. used trapping mechanism α<sub>2</sub>-macroglobulin assay for protease activity wild-type mutant produced in COS cell transfection system. found synthesized...
A protein with properties of laminin has been isolated from human placental extracts by using monoclonal antibodies.Placental tissue was extracted 0.5 M NaCl and high molecular weight proteins were the extract salt precipitation gel filtration on Sepharose 6B.The resulting fraction which contained material cross-reactive antisera to rat used as immunogen prepare hybridomas.Thirteen hybrids produced antibodies reacted basement membrane-associated antigens in indirect immunofluorescence...
Changes in cell shape are a morphological hallmark of differentiation. In this study we report that the expression ADAM12, disintegrin and metalloprotease, dramatically affects morphology preadipocytes, changing them from flattened, fibroblastic appearance to more rounded shape. We showed highest levels ADAM12 mRNA were detected preadipocytes at critical stage when become permissive for adipogenic Furthermore, as assessed by immunostaining, was transiently expressed surface concomitant with...
Abstract As in developmental and regenerative processes, cell survival is of fundamental importance cancer. Thus, a tremendous effort has been devoted to dissecting the molecular mechanisms involved understanding resistance tumor cells programmed death. Recently, stromal fibroblasts initiation progression elucidated. Here, we show that apoptosis occurs human breast carcinoma but only rarely seen nonmalignant lesions. Furthermore, ADAM12, disintegrin metalloprotease up-regulated cancer,...
Abstract Purpose: We have previously found ADAM12, a disintegrin and metalloprotease, to be an interesting biomarker for breast cancer. The purpose of this study was determine the gene protein expression profiles ADAM12 in different grades stages bladder Experimental Design: evaluated tumors from 96 patients with cancer using customized Affymetrix GeneChip. Gene validated reverse transcription-PCR, quantitative PCR, situ hybridization. Protein by immunohistochemical staining on tissue arrays...
A large number of glucagon-like-peptide-1 (GLP-1)- and peptide-YY (PYY)-producing L cells are located in the colon, but little is known about their contribution to whole body metabolism. Since bile acids (BAs) increase GLP-1 PYY release, since BAs spill over from ileum we decided investigate ability stimulate colonic secretion. Using isolated perfused rat/mouse colon as well stimulation rat vivo, demonstrate that significantly enhance secretion with average increases 3.5- 2.9-fold,...
The ADAMs (adisintegrinand metalloprotease) are a family of multidomain proteins that believed to play key roles in cell-cell and cell-matrix interactions. We have shown recently human ADAM 12-S (meltrin α) is an active metalloprotease. It synthesized as zymogen, with the prodomain maintaining protease latent form. now provide evidence latency mechanism 12 can be explained by cysteine switch model, which coordination Zn<sup>2+</sup> site catalytic domain residue critical for inhibition...
Tetranectin is a protein shared by the blood and extracellular matrix. composed of four identical, noncovalently bound polypeptides each with molecular mass approximately 21 kD. There some evidence that tetranectin may be involved in fibrinolysis proteolysis during tissue remodeling, but its precise biological function not known. enriched cartilage shark, gene expression pattern mammalian skeletal system has been determined. In present study we have examined putative osteogenesis. newborn...
The ADAMs (adisintegrinand metalloprotease) comprise a large family of multidomain proteins with cell-binding and metalloprotease activities. ADAM12 cysteine-rich domain (rADAM12-cys) supports cell attachment using syndecan-4 as primary surface receptor that subsequently triggers β<sub>1</sub>integrin-dependent spreading, stress fiber assembly, focal adhesion formation. This process contrasts on fibronectin, which is integrin-initiated but syndecan-4-dependent. In the present study, we...
Abstract Objectives The concentration of bioavailable insulin‐like growth factor (IGF) I and II is important to foetal growth. It regulated by binding proteins (IGFBP) 1 through 6. Proteolytic cleavage IGFBP‐3 takes place in human pregnancy serum; accordingly, serum levels decrease markedly during pregnancy. ADAM12 (A disintegrin m etalloprotease) an IGFBP‐5 protease present serum. goal this study was determine whether maternal a useful indicator health. Methods We developed enzyme‐linked...
The correlation between serum alpha‐foetoprotein (AFP) and the clinical pathological finding of 24 germ cell tumours arising from testes (14 cases), ovaries (3 mediastinum retroperitoneal region (2 sacrococcygeal cases) are presented. Irrespective marked differences in age sex patients, primary site outcome, cases constituted a homogeneous group fundamental histological patterns AFP synthesis. In all endodermal sinus tumour or teratocarcinomas with distinct vitelline component an increased...