A5001440213- Cell Adhesion Molecules Research
- HER2/EGFR in Cancer Research
- PARP inhibition in cancer therapy
- Toxin Mechanisms and Immunotoxins
- Mitochondrial Function and Pathology
- Cellular Mechanics and Interactions
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Calcium signaling and nucleotide metabolism
- RNA Research and Splicing
- Photoreceptor and optogenetics research
- Muscle Physiology and Disorders
- Cancer Cells and Metastasis
- Monoclonal and Polyclonal Antibodies Research
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Cancer Immunotherapy and Biomarkers
- Developmental Biology and Gene Regulation
- MicroRNA in disease regulation
- Blood disorders and treatments
- thermodynamics and calorimetric analyses
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
Kansas State University
2014-2024
National Heart Lung and Blood Institute
1992-1999
National Institutes of Health
1992-1999
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
1986-1993
The ADAMs (a disintegrin and metalloprotease) family of proteins is involved in a variety cellular interactions, including cell adhesion ecto- domain shedding. Here we show that ADAM 12 binds to surface syndecans. Three forms recombinant were used these experiments: the cys-teine-rich made Escherichia coli (rADAM 12-cys), disintegrin-like cysteine-rich insect cells 12-DC), full-length human 12-S tagged with green fluorescent protein mammalian 12-GFP). Mesenchymal specifically dose-dependent...
Satellite cells, which are skeletal muscle stem divide to provide new myonuclei growing fibers during postnatal development, and then maintained in an undifferentiated quiescent state adult muscle. This is considered be essential for the maintenance of satellite but their molecular regulation unknown. We show that Hesr1 (Hey1) Hesr3 (Heyl) (which known Notch target genes) expressed simultaneously only cells. In single-knockout mice, no obvious abnormalities cells or regenerative potentials...
Mono-ADP-ribosylation is a reversible modification of proteins, with NAD:arginine ADP-ribosyltransferases (EC 2.4.2.31) and ADP-ribosylarginine hydrolases 3.2.2.19) catalyzing the opposing reactions in an ADP-ribosylation cycle. A membrane-associated arginine-specific (mono)-ADP-ribosyltransferase was purified 215,000-fold from rabbit skeletal muscle. On basis amino acid sequences HPLC-purified tryptic peptides, degenerate oligonucleotide primers were synthesized used polymerase chain...
An arginine-specific mono-ADP-ribosyltransferase is expressed on the surface of differentiated mouse skeletal muscle cells and anchored in membrane via a glycosylphosphatidylinositol tail. Following incubation intact with [adenylate-32P]NAD analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), 97-kDa [32P]ADP-ribosylated protein was observed under reducing conditions 140-kDa complex nonreducing conditions. The ADP-ribosylated purified laminin affinity column....
Delta-like 1 (Dll1) is a mammalian ligand for Notch receptors. Interactions between Dll1 and in trans activate the pathway, whereas binding to cis inhibits signaling. undergoes proteolytic processing its extracellular domain by ADAM10. In this work we demonstrate that represents substrate several other members of ADAM family. co-transfected cells, constitutively cleaved ADAM12, N-terminal fragment released medium. ADAM12-mediated cleavage cell density-dependent, takes place orientation, does...
The clostridium-like ecto-ADP-ribosyltransferase ARTC1 is expressed in a highly restricted manner skeletal muscle and heart tissue. Although well studied, the identification of targets vivo subsequent characterization ARTC1-regulated cellular processes on proteome level have been challenging only few ARTC1-ADP-ribosylated are known. Applying our recently developed mass spectrometry-based workflow to C2C12 myotubes tissues from wild-type mice, we identify hundreds proteins whose modifications...
ClpB is a member of protein-disaggregating multi-chaperone system in Escherichia coli. The mechanism protein-folding reactions mediated by currently unknown, and the functional role different sequence regions under discussion. We have expressed purified full-length three truncated variants with N-terminal, C-terminal, double N- C-terminal deletion. studied protein concentration-dependent ATP-induced oligomerization ClpB, casein-induced activation ATPase, ClpB-assisted reactivation denatured...
ADAM12 is upregulated in human breast cancers and a predictor of chemoresistance estrogen receptor-negative tumors. induced during epithelial-to-mesenchymal transition, feature associated with claudin-low tumors, which are enriched cancer stem cell (CSC) markers. It currently unknown whether plays an active role promoting the CSC phenotype cells. expression was downregulated representative lines, SUM159PT Hs578T, using siRNA transfection or inducible shRNA expression. Cell characteristics...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTImmunological and structural conservation of mammalian skeletal muscle glycosylphosphatidylinositol-linked ADP-ribosyltransferasesIan J. Okazaki, Anna Zolkiewska, Maria S. Nightingale, Joel MossCite this: Biochemistry 1994, 33, 43, 12828–12836Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January...
ADAM12-L and ADAM12-S represent two major splice variants of human metalloproteinase-disintegrin 12 mRNA, which differ in their 3'-untranslated regions (3'UTRs). ADAM12-L, but not ADAM12-S, has prognostic chemopredictive values breast cancer. Expression levels the ADAM12 clinical samples are highly discordant, suggesting post-transcriptional regulation gene. The miR-29, miR-30, miR-200 families have potential target sites 3'UTR they may negatively regulate expression.miR-29b/c, miR-30b/d,...
ADAM 12, a member of the (protein containing disintegrin and metalloprotease) family metalloprotease-disintegrins, has been implicated in differentiation fusion skeletal myoblasts, its expression is dramatically up-regulated many cancer cells. While extracellular portion 12 contains an active metalloprotease cell-adhesion domain, function cytoplasmic much less clear. In this paper, we show that tail mediates interactions with non-receptor protein tyrosine kinase Src. The interaction direct,...
Skeletal myoblasts grown in vitro and induced to differentiate either form differentiated multinucleated myotubes or give rise quiescent, undifferentiated "reserve cells" that share several characteristics with muscle satellite cells. The mechanism of determination reserve cells is poorly understood. We find the expression level metalloprotease disintegrin ADAM12 much higher proliferating C2C12 than myotubes. Inhibition differentiating cultures by small interfering RNA accompanied lower...
Deletion of a single glutamate in torsinA correlates with early-onset dystonia, the most severe form neurological disorder characterized by uncontrollable muscle contractions. TorsinA is targeted to ER (endoplasmic reticulum) eukaryotic cells. We investigated processing and membrane association dystonia-associated Glu-deletion mutant (torsinAdeltaE). found that signal sequence (residues 1-20 from 40 amino-acid long N-terminal hydrophobic region) cleaved Drosophila S2 cells, as shown...
Myogenic cells have the ability to adopt two divergent fates upon exit from cell cycle: differentiation or self-renewal. The Notch signaling pathway is a well-known negative regulator of myogenic differentiation. Using mouse primary myoblasts cultured in vitro C2C12 cells, we found that activity essential for maintaining expression Pax7, transcription factor associated with self-renewal lineage, quiescent undifferentiated after they cycle. Stimulation by constitutively active Notch-1,...
Metalloprotease-disintegrin ADAM12 is overexpressed and frequently mutated in breast cancer. We report here that expression cultured mammalian cells up-regulated by Notch signals. Expression of a constitutively active form Notch1 murine fibroblasts, myoblasts, or mammary epithelial activation the endogenous signaling co-culture with ligand-expressing increases protein mRNA levels. Up-regulation requires new transcription, activated CSL-dependent manner, abolished upon inhibition IκB kinase....
ADAM 12, a member of the family transmembrane metalloprotease-disintegrins, has been implicated previously in differentiation skeletal myoblasts. In present study, we show that cytoplasmic tail mouse 12 interacts vitro and vivo with Src homology 3 domain p85alpha regulatory subunit phosphatidylinositol (PI) 3-kinase. By site-directed mutagenesis, have identified three p85alpha-binding sites involving PXXP motifs located at amino acids 825-828, 833-836, 884-887. Using green fluorescent...