A5068343254- Neurobiology and Insect Physiology Research
- Antimicrobial Peptides and Activities
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Medicinal Plant Research
- Cancer-related Molecular Pathways
- RNA modifications and cancer
- DNA Repair Mechanisms
- Ion Channels and Receptors
- Microtubule and mitosis dynamics
- Protein Structure and Dynamics
- Plant Stress Responses and Tolerance
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Ion channel regulation and function
- Meat and Animal Product Quality
- Insect and Pesticide Research
University of Kansas Medical Center
2021-2024
Kansas State University
2017-2021
The transient receptor potential melastatin (TRPM) tetrameric cation channels are involved in a wide range of biological functions, from temperature sensing and taste transduction to regulation cardiac function, inflammatory pain, insulin secretion. structurally conserved TRPM cytoplasmic domains make up >70 % the total protein. To investigate mechanism by which contribute gating, we employed electrophysiology cryo-EM study TRPM5-a channel that primarily relies on activation via intracellular Ca
Manduca sexta stress response peptide-2 (SRP2) is predicted to be a 25-residue peptide (FGVKDGKCPSGRVRRLGICVPDDDY), which may function as an insect cytokine regulate immune responses. Produced inactive precursor, endogenous proSRP2 probably converted active SRP2 by limited proteolysis in invading pathogens, along with prophenoloxidase and pro-Spätzle activation. In addition immunity, control head morphogenesis or other developmental processes the lepidopteran insect. We have examined...
In response to stress conditions such as wounding or infections in insects, several short peptides are processed act cytokines that induce AMP gene expression. To study their structure-activity relationship, immune inducibility, tissue specificity, responsiveness, and development relatedness, we chemically synthesized Manduca sexta peptide-1, a 25-residue peptide with one disulfide bond (SRP1: FGVRVGTCPSGYVRRGTFCFPDDDY). Upon injection of the SRP1 into naïve larvae, antimicrobial genes were...
Abstract The Transient Receptor Potential Melastatin (TRPM) family of tetrameric cation channels is involved in a wide range biological functions, from sensory perception to cardiac function. structurally conserved TRPM cytoplasmic domains make up > 70 % the total protein. To investigate mechanism by which contribute gating, we employed electrophysiology and cryo-EM study TRPM5 – channel that primarily relies on activation via intracellular Ca 2+ . Here show mammalian strongly altered...
With the genome sequence of Manduca sexta available, we have identified ten open reading frames coding for precursors paralytic peptide (PP) homologs: uENF1, uENF2, and SRP1–8. While uENF1 uENF2 are located upstream PP gene, temporarily call other eight stress responsive peptides (SRPs), since is broader than infection, a type biotic stresses. SRP2 predicted to be 25‐residue (FGVKDGKCPSGRVRRLGICVPDDDY) stabilized with disulfide bond. The proteolytic activation proSRP2 likely linked serine...
Abstract Intrinsically disordered proteins (IDPs) are key components of regulatory networks that control crucial aspects cell decision making. The intrinsically transactivation domain (TAD) tumor suppressor p53 mediates its interactions with multiple pathways to the homeostasis during cellular response genotoxic stress. Many cancer-associated mutations have been discovered in p53-TAD, but their structural and functional consequences poorly understood. Here, by combining atomistic...
Abstract SKD3, also known as human CLPB, belongs to the AAA+ family of A TPases a ssociated with various ctivities. Mutations in SKD3/CLPB gene cause 3-methylglutaconic aciduria type VII and congenital neutropenia. SKD3 is upregulated acute myeloid leukemia, where it contributes anti-cancer drug resistance. resides mitochondrial intermembrane space, forms ATP-dependent high-molecular weight complexes, but its biological function mechanistic links clinical phenotypes are currently unknown....