Background The bacterial luciferase (lux) gene cassette consists of five genes (luxCDABE) whose protein products synergistically generate bioluminescent light signals exclusive supplementary substrate additions or exogenous manipulations. Historically expressible only in prokaryotes, the lux operon was re-synthesized through a process multi-bicistronic, codon-optimization to demonstrate for first time self-directed bioluminescence emission mammalian HEK293 cell line vitro and vivo....

Pseudomonas fluorescens HK44 represents the first genetically engineered microorganism approved for field testing in United States bioremediation purposes. Strain harbors an introduced lux gene fused within a naphthalene degradative pathway, thereby allowing this recombinant microbe to bioluminesce as it degrades specific polyaromatic hydrocarbons such naphthalene. The process can therefore be monitored by detection of light. P. was inoculated into vadose zone intermediate-scale,...

ABSTRACT An estrogen-inducible bacterial lux -based bioluminescent reporter was developed in Saccharomyces cerevisiae for applications chemical sensing and environmental assessment of estrogen disruptor activity. The strain, designated S. BLYES, constructed by inserting tandem response elements between divergent yeast promoters GPD ADH1 on pUTK401 (formerly pUA12B7) that constitutively express luxA luxB to create pUTK407. Cotransformation this plasmid with a second (pUTK404) containing the...

Bacteriophages occur in high numbers environmental ecosystems and are thus significant mediators of microbial survival activities. However, interactions between populations phages situ have been largely ignored. Current understanding the process relies on studies performed with well-fed, laboratory-grown host bacteria. The purpose experiments reported here was to determine bacteriophage-host under environmentally relevant conditions nutrient limitation. These revealed importance a phenomenon...

Whole-cell, genetically modified bioreporters are designed to emit detectable signals in response a target analyte or related group of analytes. When integrated with transducer capable measuring those signals, biosensor results that acts as self-contained analytical system useful basic and applied environmental, medical, pharmacological, agricultural sciences. Historically, these devices have focused on signaling proteins such green fluorescent protein, aequorin, firefly luciferase, and/or...

Background Expression of autonomous bioluminescence from human cells was previously reported to be impossible, suggesting that all bioluminescent-based mammalian reporter systems must therefore require application a potentially influential chemical substrate. While this disproven when the bacterial luciferase (lux) cassette demonstrated function in cell, its expression required multiple genetic constructs, functional only single cell type, and generated significantly reduced signal compared...

The luxA, B, C, D, and E genes from Photorhabdus luminescens were cloned functionally expressed in Saccharomyces cerevisiae to construct a bacterial lux-based yeast bioreporter capable of autonomous bioluminescence emission. was engineered using series pBEVY expression vectors that allowed for bi-directional constitutive or inducible the individual genes. luxD gene, encoding acyl-ACP transferase ultimately supplies requisite aldehyde substrate bioluminescent reaction, fused internal...

As the use of genetically engineered microorganisms for agricultural tasks becomes more frequent, ability bacteria to exchange genetic material in setting must be assessed. Transduction (bacterial virus-mediated horizontal gene transfer) is a potentially important mechanism transfer natural environments. This study investigated potential plant leaves act as surfaces on which transduction can take place among microorganisms. Pseudomonas aeruginosa and its generalized transducing bacteriophage...

Pseudolysogeny is an environmental condition in which the starved bacterial cell coexists unstable relationship with infecting viral genomes. As nutrients are supplied to bacterium, pseudolysogens resolve into either true lysogeny or active production of virions. The direct result pseudolysogenic relationships extension effective phage half-lives natural environments. In this paper a continuous culture model interactions between host organisms and bacteriophages leading pseudolysogeny...

Bioluminescent and fluorescent reporter systems have enabled the rapid continued growth of optical imaging field over last two decades. Of particular interest has been noninvasive signal detection from mammalian tissues under both cell culture whole animal settings. Here we report on advantages limitations using a recently introduced bacterial luciferase (lux) system engineered for increased bioluminescent expression in cellular environment. Comparison with firefly (Luc) green protein...

A pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer both chromosomal and plasmid DNA between strains Pseudomonas aeruginosa. Several alleles three different P. aeruginosa were found to transduce at frequencies 10(-8) 10(-10) transductants per PFU multiplicities infection (MOI) 0.1 1. Transduction certain increased up 1000-fold as MOIs decreased 0.01. UT1 also indigenous populations microorganisms in...

Aim: To incorporate into the lambda phage genome, a luxI‐based acyl‐homoserine lactone (AHL) synthase genetic construct and exploit autoamplified power of quorum sensing to translate infection event chemical signature detectable by lux‐based bioluminescent bioreporter, with focus towards facile detection microbial pathogens. Methods Results: The luxI gene from Vibrio fischeri was inserted genome model phage‐based biosensor system for general Escherichia coli. AHL signalling molecules...