A5010447231- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Legionella and Acanthamoeba research
- Plant and Fungal Interactions Research
- Viral gastroenteritis research and epidemiology
- Genomics and Phylogenetic Studies
- Parvovirus B19 Infection Studies
- Infection Control and Ventilation
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Virology and Viral Diseases
- SARS-CoV-2 detection and testing
- Legume Nitrogen Fixing Symbiosis
- Parasitic Infections and Diagnostics
- COVID-19 epidemiological studies
- Cancer Research and Treatments
- Bacillus and Francisella bacterial research
Universidade Federal de Minas Gerais
2018-2024
Since the discovery of giant viruses infecting amoebae in 2003, many dogmas virology have been revised and search for these has intensified. Over last few years, several new groups discovered various types samples environments.In this work, we describe isolation 68 amoeba obtained from environmental Brazil Antarctica. Isolated were identified by hemacolor staining, PCR assays electron microscopy (scanning and/or transmission). A total 64 belonging to Mimiviridae family isolated (26 lineage...
Here we report the discovery of Yaravirus, a lineage amoebal virus with puzzling origin and evolution. Yaravirus presents 80-nm-sized particles 44,924-bp dsDNA genome encoding for 74 predicted proteins. annotation showed that none its genes matched sequences known organisms at nucleotide level; amino acid level, six proteins had distant matches in nr database. Complimentary prediction three-dimensional structures indicated possible function 17 total. Furthermore, were not able to retrieve...
ABSTRACT Giant viruses with their gigantic genomes are among the most intriguing components of virosphere. How these attained such giant remains unclear, despite considerable efforts to understand this phenomenon. Here, we describe discovery cedratvirus pambiensis, an amoebal virus isolated in Brazil. Although virion morphology and replication cycle c. pambiensis very similar those described for other cedratviruses, whole genome sequencing revealed largest ever described, 623,564 base pairs...
Giant viruses have been isolated and characterized in different environments, expanding our knowledge about the biology of these unique microorganisms. In last 2 years, a new group was discovered, cedratviruses, currently composed only two isolates members putative family, "Pithoviridae," along with previously known pithoviruses. Here we report isolation biological genomic characterization novel cedratviruses from samples collected France Brazil. Both were using Acanthamoeba castellanii as...
Among the most intriguing structural features in known virosphere are mimivirus surface fibrils, proteinaceous filaments approximately 150 nm long, covering capsid surface. Fibrils important to promote particle adhesion host cells, triggering phagocytosis and cell infection. However, although mimiviruses one of abundant viral entities a plethora biomes worldwide, there has been no comparative analysis on fibril organization abundance among distinct isolates. Here, we describe isolation...
This article describes a practical method for prospecting and isolating giant viruses based on direct inoculation of environmental samples into amoeba cultures Acanthamoeba castellanii. The that infect amoebas have already been isolated from various in several countries worldwide, including extreme environments. Here we describe the methodologic procedures regarding A. castellanii, preparation samples, culture amoebas, observation cytopathic effects can indicate presence potential isolation...
Abstract Here we report the discovery of Yaravirus, a new lineage amoebal virus with puzzling origin and phylogeny. Yaravirus presents 80 nm-sized particles 44,924 bp dsDNA genome encoding for 74 predicted proteins. More than 90% (68) genes have never been described before, representing ORFans. Only six had distant homologs in public databases: an exonuclease/recombinase, packaging-ATPase, bifunctional DNA primase/polymerase three hypothetical Furthermore, were not able to retrieve viral...
Marseilleviruses (MsV) are a group of viruses that compose the Marseilleviridae family within Nucleocytoviricota phylum. They have been found in different samples, mainly freshwater. MsV classically organized into five phylogenetic lineages (A/B/C/D/E), but current taxonomy does not fully represent all diversity lineages. Here, we describe novel strain isolated from Brazilian saltwater sample named Marseillevirus cajuinensis. Based on genomics and analyses, M. cajuinensis exhibits 380,653-bp...