A5019317461- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Legionella and Acanthamoeba research
- Parasitic Infections and Diagnostics
- Viral gastroenteritis research and epidemiology
- Polyomavirus and related diseases
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Viral Infections and Vectors
- Animal Virus Infections Studies
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- Microbial infections and disease research
- Toxoplasma gondii Research Studies
- Vibrio bacteria research studies
- Plant and Fungal Interactions Research
- Vector-borne infectious diseases
- Parasite Biology and Host Interactions
- Viral Infections and Immunology Research
- Bacterial biofilms and quorum sensing
- Helminth infection and control
- Parasites and Host Interactions
- Bacillus and Francisella bacterial research
- Genomics and Phylogenetic Studies
Universidade Federal de Minas Gerais
2013-2019
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2018-2019
Instituto de Ensino e Pesquisa Santa Casa
2019
Centro Infantil Boldrini
2019
Yale New Haven Health System
2018
Universidade de São Paulo
2012-2016
Institut de Recherche pour le Développement
2014-2015
Centre National de la Recherche Scientifique
2014-2015
Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes
2015
Aix-Marseille Université
2014-2015
The identification of novel giant viruses from the nucleocytoplasmic large DNA group and their virophages has increased in last decade helped to shed light on viral evolution. This study describe discovery, isolation characterization Samba virus (SMBV), a belonging Mimivirus genus, which was isolated Negro River Brazilian Amazon. We also report an SMBV-associated virophage named Rio (RNV), is first be Americas.Based phylogenetic analysis, SMBV belongs A putative Megavirales order, possibly...
ABSTRACT Acanthamoeba polyphaga mimivirus (APMV) is a giant virus from the Mimiviridae family. It has many unusual features, such as pseudoicosahedral capsid that presents starfish shape in one of its vertices, through which ∼1.2-Mb double-stranded DNA released. also dense glycoprotein fibril layer covering not yet been functionally characterized. Here, we verified although these structures are essential for viral replication, they truly necessary adhesion to amoebae, natural host. In...
Hookworm infection causes anemia, malnutrition, and growth delay, especially in children living sub-Saharan Africa. The World Health Organization recommends periodic mass drug administration (MDA) of anthelminthics to school-age (SAC) as a means reducing morbidity. Recently, questions have been raised about the effectiveness MDA global control strategy for hookworms other soil-transmitted helminths (STHs). Genomic DNA was extracted from Necator americanus hookworm eggs isolated SAC enrolled...
The Megavirales are a newly described order capable of infecting different types eukaryotic hosts. For the most part, natural host is unknown. Several methods have been used to detect these viruses, with large discrepancies between molecular and co-cultures. To isolate giant we propose use species amoeba as cellular support. aim this work was new Brazilian viruses by comparing protozoa Acanthamoeba castellanii, A. polyphaga, griffini, Vermamoeba vermiformis (VV) platform for isolation using...
Giant viruses of amoebae are distinct from classical by the giant size their virions and genomes. Pandoraviruses record holders in genomes number predicted genes. Three strains, P. salinus, dulcis, inopinatum, have been described to date. We isolated three new ones, namely massiliensis, braziliensis, pampulha, environmental samples collected Brazil. describe here genomes, transcriptome proteome pangenome group encompassing six pandoravirus isolates. Genome sequencing was performed with an...
Since the discovery of giant viruses infecting amoebae in 2003, many dogmas virology have been revised and search for these has intensified. Over last few years, several new groups discovered various types samples environments.In this work, we describe isolation 68 amoeba obtained from environmental Brazil Antarctica. Isolated were identified by hemacolor staining, PCR assays electron microscopy (scanning and/or transmission). A total 64 belonging to Mimiviridae family isolated (26 lineage...
In 2003, Acanthamoeba polyphaga mimivirus (APMV) was discovered as parasitizing Acanthamoeba. It revealed to exhibit remarkable features, especially odd genomic characteristics, and founded viral family Mimiviridae. Subsequently, a second of giant amoebal viruses described, Marseilleviridae, whose prototype member is Marseillevirus, in 2009. Currently, the genomes seven different members this have been fully sequenced. Previous phylogenetic analysis suggested existence three Marseilleviridae...
The giant viruses are the largest and most complex in virosphere. In last decade, new members have constantly been added to this group. Here, we provide an in-depth descriptive analysis of replication cycle Cedratvirus getuliensis, one known date. We tracked virion entry, early steps virus factory particles morphogenesis, during phase, observed a unique sequential organization immature particle elements, including horseshoe rectangular compartments, revealed by transverse longitudinal...
Giant viruses are complex members of the virosphere, exhibiting outstanding structural and genomic features. Among these viruses, pandoraviruses some most intriguing members, giant particles genomes presenting at up to 2.5 Mb, with many genes having no known function. In this work, we analyzed, by virological microscopic methods, replication cycle steps three new pandoravirus isolates from samples collected in different regions Brazil. Our data indicate that all analyzed can deeply modify...
Since the recent discovery of Samba virus, first representative family Mimiviridae from Brazil, prospecting for mimiviruses has been conducted in different environmental conditions Brazil. Recently, we isolated using Acanthamoeba sp. three new mimiviruses, all lineage A amoebal mimiviruses: Kroon virus urban lake water; Amazonia Brazilian Amazon river; and Oyster farmed oysters. The aims this work were to sequence analyze genome these (mimi-BR) update analysis genome. genomes 97%-99%...
To investigate circulation of mimiviruses in the Amazon Region Brazil, we surveyed 513 serum samples from domestic and wild mammals. Neutralizing antibodies were detected 15 sample pools, mimivirus DNA was 9 pools capuchin monkeys 16 cattle.
We report a 3-year-old child who was hospitalized because of severe manifestations the central nervous system. The died after 6 days hospitalization. Analysis postmortem cerebrospinal fluid showed presence yellow fever virus RNA. Nucleotide sequencing confirmed that wild-type virus.
Since Acanthamoeba polyphaga mimivirus was identified in 2003, several other giant viruses of amoebae have been isolated, highlighting the uniqueness this group. In context, tupanviruses were recently isolated from extreme environments Brazil, presenting virions with an outstanding tailed structure and genomes containing most complete set translation genes virosphere. Unlike amoebae, present a broad host range, being able to replicate not only sp. but also such as Vermamoeba vermiformis,...
Viruses are extremely diverse and abundant present in countless environments. Giant viruses of the Megavirales order have emerged as a fascinating research topic for virologists around world. As evidence their ubiquity ecological impact, mimiviruses been found multiple environmental samples. However, isolation these from samples is inefficient, mainly due to methodological limitations lack information regarding interactions between substrates. In this work, we demonstrate long-lasting...
Viruses depend on cells to replicate and can cause considerable damage their hosts. However, hosts have developed a plethora of antiviral mechanisms counterattack or prevent viral replication maintain homeostasis. Advantageous features are constantly being selected, affecting host-virus interactions constituting harsh race for supremacy in nature. Here, we describe new mechanism unveiled by the interaction between giant virus its amoebal host. Faustovirus mariensis infects Vermamoeba...
The complexity of giant virus genomes is intriguing, especially the presence genes encoding components protein translation machinery such as transfer RNAs and aminoacyl-tRNA-synthetases; these features are uncommon among other viruses. Although orthologs codified by their hosts, one can hypothesize that having translation-related might represent a gain fitness during infection. Therefore, aim this study was to evaluate expression mimivirus infection Acanthamoeba castellanii under different...
The family Mimiviridae, comprised by giant DNA viruses, has been increasingly studied since the isolation of Acanthamoeba polyphaga mimivirus (APMV), in 2003. In this work, we describe genome analysis two new mimiviruses, each isolated from a distinct Brazilian environment. Furthermore, for first time, are reporting genomic characterization mimiviruses group C Brazil (Br-mimiC), where predominance A previously reported. genomes Br-mimiC isolates Mimivirus gilmour (MVGM) and golden (MVGD)...
The inclusion of Mimiviridae members in the putative monophyletic nucleocytoplasmic large DNA virus (NCLDV) group is based on genomic and phylogenomic patterns. This shows that, along with other viral families, they share a set genes known as core or "hallmark genes," including gene for major capsid protein (MCP). Although previous studies have suggested that maturation mimivirus MCP transcripts dependent splicing, there little information about processing this transcript isolates. Here we...