A5003075346- Receptor Mechanisms and Signaling
- Protein Kinase Regulation and GTPase Signaling
- Protein Structure and Dynamics
- Mass Spectrometry Techniques and Applications
- Neuropeptides and Animal Physiology
- Computer Graphics and Visualization Techniques
- Advanced Vision and Imaging
- Monoclonal and Polyclonal Antibodies Research
- Medical Image Segmentation Techniques
- Metabolism, Diabetes, and Cancer
- Survey Sampling and Estimation Techniques
- 3D Shape Modeling and Analysis
- Advanced Image and Video Retrieval Techniques
- Cellular transport and secretion
- Extracellular vesicles in disease
- Autophagy in Disease and Therapy
- Music Technology and Sound Studies
- Adenosine and Purinergic Signaling
Sungkyunkwan University
2020-2024
Abstract Heterotrimeric G proteins are categorized into four main families based on their function and sequence, Gs, Gi/o, Gq/11, G12/13. One receptor can couple to more than one protein subtype, the coupling efficiency varies depending GPCR-G pair. However, precise mechanism underlying different efficiencies is unknown. Here, we study structural primary secondary Gi/o coupling, using muscarinic acetylcholine type 2 (M2R) as Gi/o-coupling β -adrenergic (β AR, which primarily couples Gs)...
G proteins are major signaling partners for protein-coupled receptors (GPCRs). Although stepwise structural changes during GPCR–G protein complex formation and guanosine diphosphate (GDP) release have been reported, no information is available with regard to triphosphate (GTP) binding. Here, we used a novel Bayesian integrative modeling framework that combines data from hydrogen-deuterium exchange mass spectrometry, tryptophan-induced fluorescence quenching, metadynamics simulations derive...
Abstract The application of extracellular vesicles (EVs) as vehicles for anti‐Parkinson's agents represents a significant advance, yet their clinical translation is hampered by challenges in efficient brain delivery and complex blood‐brain barrier (BBB) targeting strategies. In this study, we engineered dopamine onto the surface adipose‐derived stem cell EVs (Dopa‐EVs) utilizing facile, two‐step cross‐linking approach. This engineering enhanced neuronal uptake primary neurons neuroblastoma...
Heterotrimeric guanine nucleotide-binding proteins (G proteins) are composed of α, β, and γ subunits, Gα has a GDP/GTP-binding pocket. When nucleotide exchange factor (GEF) interacts with Gα, GDP is released, GTP to Gα. The GTP-bound activated dissociates from GEF Gβγ, mediating the induction various intracellular signaling pathways. Depending on sequence similarity cellular function, subunits subcategorized into four subfamilies: Gαi/o, Gαs, Gαq/11, Gα12/13. Although Gαi/o subtype family...
Existing score-distilling text-to-3D generation techniques, despite their considerable promise, often encounter the view inconsistency problem. One of most notable issues is Janus problem, where canonical an object (\textit{e.g}., face or head) appears in other views. In this work, we explore existing frameworks for and identify main causes problem -- embedded bias 2D diffusion models. Based on these findings, propose two approaches to debias score-distillation view-consistent generation....
Recent studies have demonstrated that diffusion models are capable of generating high-quality samples, but their quality heavily depends on sampling guidance techniques, such as classifier (CG) and classifier-free (CFG). These techniques often not applicable in unconditional generation or various downstream tasks image restoration. In this paper, we propose a novel guidance, called Perturbed-Attention Guidance (PAG), which improves sample across both conditional settings, achieving without...
Score distillation sampling (SDS), the methodology in which score from pretrained 2D diffusion models is distilled into 3D representation, has recently brought significant advancements text-to-3D generation task. However, this approach still confronted with critical geometric inconsistency problems such as Janus problem. Starting a hypothesis that may be induced by multiview inconsistencies between scores predicted various viewpoints, we introduce GSD, simple and general plug-and-play...
Diffusion models excel in generating high-quality images. However, current diffusion struggle to produce reliable images without guidance methods, such as classifier-free (CFG). Are methods truly necessary? Observing that noise obtained via inversion can reconstruct guidance, we focus on the initial of denoising pipeline. By mapping Gaussian `guidance-free noise', uncover small low-magnitude low-frequency components significantly enhance process, removing need for and thus improving both...
SUMMARY G proteins are major signaling partners for protein-coupled receptors (GPCRs). Although stepwise structural changes during GPCR–G protein complex formation and guanosine diphosphate (GDP) release have been reported, no information is available with regard to triphosphate (GTP) binding. Here, we used a novel Bayesian integrative modeling framework that combines data from hydrogen-deuterium exchange mass spectrometry, tryptophan-induced fluorescence quenching, metadynamics simulations...