A5069729642- Sarcoma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Soft tissue tumor case studies
- Soft tissue tumors and treatment
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Chromatin Remodeling and Cancer
- Advanced MRI Techniques and Applications
- Histiocytic Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Advanced NMR Techniques and Applications
- Cancer and Skin Lesions
- Protein Degradation and Inhibitors
- NMR spectroscopy and applications
- Peptidase Inhibition and Analysis
- Histone Deacetylase Inhibitors Research
- Vascular Malformations and Hemangiomas
- Genomic variations and chromosomal abnormalities
- Advanced Breast Cancer Therapies
- Metal-Catalyzed Oxygenation Mechanisms
Memorial Sloan Kettering Cancer Center
2009-2023
Sarcoma Oncology Center
2006-2023
Kettering University
2012-2022
Rockefeller University
2011
Louisiana State University System
2011
Pennington Biomedical Research Center
2011
Temple Street Children's University Hospital
2004
Surface-enhanced resonance Raman scattering gold nanostars allow detection of macro- and microscopic foci premalignant cancerous lesions in vivo.
Abstract Classification of liposarcoma into three biological types encompassing five subtypes, (a) well-differentiated/dedifferentiated, (b) myxoid/round cell, and (c) pleomorphic, based on morphologic features cytogenetic aberrations, is widely accepted. However, diagnostic discordance remains even among expert sarcoma pathologists. We sought to develop a more systematic approach classification gene expression analysis identify subtype-specific differentially expressed genes that may be...
CTNNB1 mutations or APC abnormalities have been observed in ∼85% of desmoids examined by Sanger sequencing and are associated with Wnt/β‐catenin activation. We sought to identify molecular aberrations “wild‐type” tumors (those without alteration) determine their prognostic relevance. was 117 desmoids; a mutation 101 (86%) 16 were wild type. Wild‐type status did not associate tumor recurrence. Moreover, unsupervised clustering based on U133A‐derived gene expression profiles, wild‐type mutated...
// Marta Kovatcheva 1,5,* , David D. Liu 2,5,* Mark A. Dickson 3,7 Mary E. Klein 1,5 Rachael O’Connor 8 Fatima O. Wilder Nicholas Socci 6 William Tap Gary K. Schwartz 7,9 Samuel Singer Aimee M. Crago 5,8 and Andrew Koff 1,2,5 1 The Louis V. Gerstner Graduate School of Biomedical Sciences, Sloan-Kettering Institute, Memorial Cancer Center, New York, USA 2 Program in Biochemistry, Cellular Molecular Biology, Weill College Medicine, Cornell University, 3 Department 4 Surgery, 5...
Liposarcoma remains the most common mesenchymal cancer, with a mortality rate of 60% among patients this disease. To address present lack therapeutic options, we embarked upon study microRNA (miRNA) expression alterations associated liposarcomagenesis goal exploiting differentially expressed miRNAs and gene products they regulate as potential targets. MicroRNA was profiled in samples normal adipose tissue, well-differentiated liposarcoma, dedifferentiated liposarcoma by both deep sequencing...
Liposarcomas are the most common type of soft tissue sarcoma but their genetics poorly defined. To identify genes that contribute to liposarcomagenesis and serve as prognostic candidates, we undertook expression profiling 140 primary liposarcoma samples, which were randomly split into training set (n = 95) test 45). A multigene predictor for distant recurrence-free survival (DRFS) was developed by supervised principal component method. Expression levels 588 in used calculate a risk score...
Myxofibrosarcoma is a common mesenchymal malignancy with complex genomics and heterogeneous clinical outcomes. Through gene-expression profiling of 64 primary high-grade myxofibrosarcomas, we defined an expression signature associated outcome. The gene most significantly disease-specific death distant metastasis was ITGA10 (integrin-α10). Functional studies revealed that myxofibrosarcoma cells strongly depended on integrin-α10, whereas normal did not. Integrin-α10 transmitted its...
Molecular events underlying progression of well-differentiated liposarcoma (WDLS) to dedifferentiated (DDLS) are poorly defined. This study sought identify copy number alterations (CNA) associated with dedifferentiation WDLS, DDLS morphology, and patient outcomes.Fifty-five WDLS 52 were analyzed using Agilent 244K comparative genomic hybridization Affymetrix U133A expression arrays. CNAs identified by RAE analysis. Thirty-nine the specimens categorized morphologically a single...
Abstract Gastrointestinal stromal tumors (GIST) are characterized by activating mutations in the c-KIT gene which confers ligand-independent activation of KIT receptor. Imatinib mesylate has been shown to effectively block constitutively active and delay tumor growth. However, resistance imatinib is emerging as a major clinical problem novel therapies needed. We report that treatment GIST cells with transcriptional inhibitor flavopiridol, initially down-regulates antiapoptotic proteins...
Abstract High‐resolution magic‐angle‐spinning (HR‐MAS) NMR spectroscopy detects resolved signals from membrane phospholipids and proteins in intact cell tissue samples. MAS has the additional advantage of quenching spin‐diffusion through a mutual “flip‐flop” neighbor spins by time‐independent dipolar coupling as long is “inhomogeneous.” Under MAS, significant magnetization transfer (MT) was observed between water each proton site phospholipid NMR‐observable protein signals. The MT rates are...
Abstract Well‐differentiated liposarcoma (WDLS) and dedifferentiated (DDLS) represent the most common biological group of liposarcoma, there is a pressing need to develop targeted therapies for patients with advanced disease. To identify potential therapeutic targets, we sought differences in adipogenic pathways between DDLS, WDLS, normal adipose tissue. In microarray analysis DDLS ( n = 84), WDLS 79), fat 23), C/EBPα, transcription factor involved cell cycle regulation differentiation, was...
Proton NMR spectra of freshly isolated human skeletal muscle samples contain creatine and phosphocreatine resonances with distinct chemical shifts that are easily visualized magic angle spinning (MAS, the sample rapidly at 54.7 degrees respect to magnetic field) methods. The identification resonance was based on two findings: (i) possible small dipolar coupling does not contribute line splitting under rapid MAS, (ii) 1H signal decreases concurrently observed in 31P experiments. In MAS...
Abstract Purpose: This study sought to identify β-catenin targets that regulate desmoid oncogenesis and determine whether external signaling pathways, particularly those inhibited by sorafenib (e.g., PDGFRβ), affect these alter natural history or treatment response in patients. Experimental Design: In vitro experiments utilized primary cell lines examine regulation of targets. Relevance results was assessed vivo using Alliance trial A091105 correlative biopsies. Results: CTNNB1 knockdown...
<div>AbstractPurpose:<p>This study sought to identify β-catenin targets that regulate desmoid oncogenesis and determine whether external signaling pathways, particularly those inhibited by sorafenib (e.g., PDGFRβ), affect these alter natural history or treatment response in patients.</p>Experimental Design:<p><i>In vitro</i> experiments utilized primary cell lines examine regulation of targets. Relevance results was assessed <i>in vivo</i>...
<p>Supplemental Methods Tables, Supplemental Figures, Figures</p>
<div>AbstractPurpose:<p>This study sought to identify β-catenin targets that regulate desmoid oncogenesis and determine whether external signaling pathways, particularly those inhibited by sorafenib (e.g., PDGFRβ), affect these alter natural history or treatment response in patients.</p>Experimental Design:<p><i>In vitro</i> experiments utilized primary cell lines examine regulation of targets. Relevance results was assessed <i>in vivo</i>...
<p>Supplemental Methods Tables, Supplemental Figures, Figures</p>