An increasing amount of sewage sludge requires reasonable management, whereas its storage might be environmentally hazardous. Due to the organic matter and nutrient presence in sediments, it may used as fertilizer. However, beyond valuable contests, can also contain toxic or dangerous ingredients like heavy metals. Therefore, there is a need develop methods for rapid assessment sediment ecotoxicity that will determine possible applicability agriculture. The Biolog® EcoPlate enables metabolic...

The rates of N cycling and soil enzyme activities involved in the transformation N-related nutrients are rarely measured soils below a 30 cm depth, even though substantial amounts nitrogen also stored deep soils. aim this study was to determine how microbial enzymatic properties changed as function depth across profiles that were developed on same parent material but differed terms soil-forming processes. Two excavated fields with lucerne two under winter wheat. We assessed N-cycling...

The aim of the study was to develop a method for early detection and identification fungal contamination building materials using an electronic nose. Therefore, laboratory experiments based on analysis air in vicinity isolates potentially found were performed. results revealed that employed gas sensors array consisting MOS-type enables differences among examined samples fungi distinguishing between non-contaminated contaminated samples, shortly after occurs. Electronic nose readouts analysed...

Loss of organic matter content cultivated soils is observed in many regions Europe. The possibility using waste as a soil additive that enriches the with and essential components important quality protection management. This research concerned influence six wastes—two industrial composts, three digestates meat bone meal—on microbial properties. study functional diversity concerns determination catabolic capacity bacterial, fungal anaerobic communities relation to carbon substrates metabolic...

Regarding the unfavourable changes in agroecosystems resulting from excessive application of mineral fertilizers, biopreparations containing live microorganisms are gaining increasing attention. We assumed that phosphorus fertilizer enriched with strains beneficial contribute to favourable enzymatic activity and genetic functional diversity microbial populations inhabiting degraded soils. Therefore, field experiments conditions, effects bacterial on status soil microbiome two chemically...

Waste exogenous organic matter, including spent mushroom substrate (SMS) and chicken manure (CM), can be used as the basis of a soil-improving cropping system in sustainable agriculture. However, there is—as yet—a lack information about important quality indicators such fungal community relative abundance, structure biodiversity soils treated with these additives. In this study, responses soil composition mycobiome diversity to SMS CM application compared control were evaluated using...

The need for finding fungicides against Fusarium is a key step in the chemical plant protection and using appropriate agents. Existing, conventional methods of evaluation isolates resistance to are costly, time-consuming potentially environmentally harmful due usage high amounts toxic chemicals. Therefore, development fast, accurate effective detection urgently required. MT2 microplates (BiologTM) method traditionally used bacteria identification their ability utilize different carbon...

Pentachlorophenol (PCP) is a widely detectable toxic chemical with detrimental effect on the functioning of ecosystems. A field trial was undertaken to study its effects activities, metabolic and genetic diversities, as well soil bacterial fungal community composition. The seeds faba bean (Vicia L.) were either treated or left untreated (Control) pentachlorophenol before sowing. During vegetative period, samples taken three times from rhizosphere in order assess influence PCP enzymatic...

The knowledge about microorganisms—activity and diversity under hop production is still limited. We assumed that, different systems of (within the same soil climatic conditions) significantly influence on composition microbial populations its functional activity (metabolic potential). Therefore, we compared a set properties in field experiment two (a) ecological based use probiotic preparations organic fertilization (b) conventional—with chemical pesticides mineral fertilizers. Soil analyses...

Abstract The aim of the study was to assess differences in bacterial community physiological profiles soils contaminated with heavy metals versus without metal contaminations. study’s soil originated from surrounding area Szopienice non-ferrous smelter (Silesia Region, Poland). control unexposed metals. Metal concentration appraised by flame atomic absorption spectrometry, whereas community-level profile determined Biolog EcoPlates TM system. microbiological activity both sites also assessed...

Abstract Arabinogalactan proteins (AGPs) are cell components implicated in plant-microbe interactions. Despite the significance of AGPs response to stress factors, their distribution during development fungal disease fruit is unknown. In our work, situ analysis AGP arrangement inoculated with Penicillium spinulosum consecutive days infection was carried out. For immunolocalization AGPs, samples were incubated JIM13, MAC207, LM2, and LM14 antibodies recognizing carbohydrate moieties. To...

Cellulose degradation is less known in the Ascomycota phylum, but it important to recognize because this process influences most biochemical cycle: carbon cycle. carried out by a complex enzyme: cellulase. Petriella setifera has recently been recognized as producer of cellulolytic enzymes. In work, was shown, that activity cellulose-degrading enzymes, source utilization and gene expression P. were not determined pre-culturing different lignocellulosic wastes. Moreover, found presence glucose...

The rational utilisation of sludge as organic matter application into the soil permits enrichment in nutrients such nitrogen and phosphorus. As dairy sewage contains large amounts minerals, agriculture appears to be a noteworthy proposal. However, waste can also source toxic substances, heavy metals, inhibitors, xenobiotics potentially pathogenic microorganisms. Therefore, it is so important monitor its microbiological biochemical properties aspect safety for human health, natural...

The microbiological hydrolysis of lignocellulose waste materials, using enzymatic biopreparations and its anaerobic processing, is a promising strategy for the efficient use renewable energy sources. This article presents optimization media to improve cellulase production by Trichoderma atroviride G79/11 further application as cellulolytic biopreparation. characterization T. phenotypic microarrays was performed Biolog® PM plates approach. investigations were aimed at increasing efficiency...

A multi-enzymatic biopreparation of Trichoderma atroviride G79/11 origin was characterized. The fungus showed relatively high cellulase production in a soybean flour-cellulose-lactose medium. Subsequently, based on its post-culture liquid, the enzyme mixture developed and liquid form reached 22 U cm-3 cellulolytic activity lyophilisate exhibited 1.09 at pH 5.1 50 °C. characterized by following activities: xylanase, β-glucosidase, carboxymethyl cellulase, polygalactouronase, pectinesterase,...

The goal of this study was to evaluate the impact alkaline fly ash on selected microbiological properties soils in vicinity an electric power plant. specific objectives were as follows: first, determine metabolic activity and functional diversity soils, such biological indicators acidic soil; second, compare changes microbial based distance from dumping site located plant; third, profile pattern with different pH levels. revealed stimulation dehydrogenases (DHA) respiration (RESP) by ash. It...

The molecular fingerprinting methods used to evaluate soil microbial diversity could also be as effective biosensors for the purposes of monitoring ecological status. biodiversity microorganisms is a relevant index activity and there necessity develop tools generate reliable results an emerging approach in field environmental control using biosensors. This work reports method under development determining high efficiency Multiplex PCR-Terminal Restriction Fragment Length Polymorphism...

Abstract Anthropogenic‐induced deterioration of soil health remains a global problem, resulting in diminished productivity agroecosystems. In order to improve quality, we investigated the impact phosphorus biofertilizer on microbiological parameters (type Brunic Arenosol) degraded as result inappropriate cultivation and fertilization, characterized by low pH decrease K Mg content. Two‐year field experiment included control treatment (FC) without microbial enrichment FA100 (fertilizer...