Agata Leszczuk

ORCID: 0000-0002-8876-8377
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About
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Research Areas
  • Polysaccharides and Plant Cell Walls
  • Polysaccharides Composition and Applications
  • Postharvest Quality and Shelf Life Management
  • Plant Reproductive Biology
  • Plant Taxonomy and Phylogenetics
  • Plant Molecular Biology Research
  • Botanical Research and Applications
  • Enzyme Production and Characterization
  • Polar Research and Ecology
  • Aluminum toxicity and tolerance in plants and animals
  • Plant Gene Expression Analysis
  • Plant Surface Properties and Treatments
  • Plant Stress Responses and Tolerance
  • Food composition and properties
  • Tree Root and Stability Studies
  • Plant responses to water stress
  • Plant tissue culture and regeneration
  • Photosynthetic Processes and Mechanisms
  • Biocrusts and Microbial Ecology
  • Plant pathogens and resistance mechanisms
  • Microbial Metabolites in Food Biotechnology
  • Yeasts and Rust Fungi Studies
  • Botany, Ecology, and Taxonomy Studies
  • Climate change and permafrost
  • Seedling growth and survival studies

Institute of Agrophysics, Polish Academy of Sciences
2017-2025

Maria Curie-Skłodowska University
2015-2016

Arabinogalactan proteins (AGPs) are a class of heavily glycosylated occurring as structural element the cell wall-plasma membrane continuum. The features AGPs described earlier suggest that may be implicated in plant adaptation to stress conditions important developmental phases during reproduction process. In this paper, microscopic and immunocytochemical studies conducted using specific antibodies (JIM13, JIM15, MAC207) recognizing carbohydrate chains showed significant changes AGP...

10.3390/cells10081880 article EN cc-by Cells 2021-07-24

Arabinogalactan proteins (AGPs) are constituents of the plant cell walls, which increasingly being considered as essential molecules in formation extracellular matrix. Given their molecular structure and advantage carbohydrate moiety, glycosylation level its modification is assumed a cause functional diversity AGPs. The aim current work was to evaluate features AGP potential molecule involved ripening process. For this purpose, tomato fruits were analyzed at specific five stages using...

10.1016/j.scienta.2022.111718 article EN cc-by Scientia Horticulturae 2022-11-26

Abstract Arabinogalactan proteins (AGPs) are cell components implicated in plant-microbe interactions. Despite the significance of AGPs response to stress factors, their distribution during development fungal disease fruit is unknown. In our work, situ analysis AGP arrangement inoculated with Penicillium spinulosum consecutive days infection was carried out. For immunolocalization AGPs, samples were incubated JIM13, MAC207, LM2, and LM14 antibodies recognizing carbohydrate moieties. To...

10.1038/s41598-019-54022-3 article EN cc-by Scientific Reports 2019-11-22

Arabinogalactan proteins (AGPs) are constituents of the cell wall-plasma membrane continuum in fruit tissue. The aim study was to characterise AGPs contained by determination their chemical structure and morphological properties. results were obtained from ex situ investigations a comparative analysis present Malus × domestica at different stages ripening green through mature stage over-ripening during storage. HPLC colorimetric methods used for analyses composition monosaccharides extracted...

10.3390/biology9080225 article EN cc-by Biology 2020-08-14

Arabinogalactan proteins (AGPs) are proteoglycans with an unusual molecular structure characterised by the presence of a protein part and carbohydrate chains. Their specific properties at different stages fruit ripening programme make AGPs unique markers this process. An important function is to co-form amorphous extracellular matrix in cell wall-plasma membrane continuum; thus, changes these molecules can determine distribution other components. The aim current work was characterise...

10.3389/fpls.2024.1365490 article EN cc-by Frontiers in Plant Science 2024-03-20

Changes in the arrangement of cell wall components determine properties (integrity, stiffness), thereby affecting macro-scale fruits, which are important for consumers and industry. Arabinogalactan proteins (AGPs) ubiquitous plant cell, they have various functions. Currently, AGPs considered to be one less well-known, enigmatic proteoglycans, a consequence their heterogeneous structure unclear mechanism activity. An immunocytochemical study was conducted elucidate distribution pectic...

10.1093/aob/mcy133 article EN Annals of Botany 2018-06-15

Abstract Arabinogalactan proteins (AGPs) are ubiquitous cell wall and plasma membrane components characterised by extensive glycosylation heterogeneity of their carbohydrate protein units. The aim the study was to evaluate structural features AGPs present in apple fruits at different stages ripening process. were extracted using Yariv reagent examined SDS-PAGE, immunoblotting, FT-IR, AFM. In situ analysis, immunofluorescence (CLSM) immunogold-labelling (TEM), performed. We demonstrated that...

10.1038/s41598-020-77749-w article EN cc-by Scientific Reports 2020-11-26

In this study, the presence of calcium oxalate crystals and distribution arabinogalactan proteins (AGPs) in ovary Fragaria x ananassa (Duchesne) were investigated. The arrangement AGP epitopes was analyzed using immunohistochemistry techniques with JIM13, JIM15, MAC207 monoclonal antibodies. To confirm immunofluorescence studies, experiments at subcellular level performed immunogold method transmission electron microscopy. We observed as druses middle layer ovary, which created a distinct...

10.1007/s00344-018-09912-7 article EN cc-by Journal of Plant Growth Regulation 2019-01-03

Abstract Ripening is a process involving various morphological, physiological, and biochemical changes in fruits. This affected by modifications the cell wall structure, particularly composition of polysaccharides proteins. The assembly network proteoglycans named arabinoxylan pectin arabinogalactan protein1 (APAP1). complex consists protein (AGP) core with domain including (AG) type II, homogalacturonan (HG), rhamnogalacturonan I (RG-I). present paper aims to determine impact disturbance...

10.1093/hr/uhae145 article EN cc-by Horticulture Research 2024-05-24

Abstract Background Oxygen concentration is a key characteristic of the fruit storage environment determining shelf life and quality. The aim work was to identify cell wall components that are related response low oxygen conditions in determine effects such on ripening process. Tomato ( Solanum lycopersicum) fruits at different stages process were stored an anoxic hypoxic environment, 0% 5% concentrations, respectively. We used comprehensive comparative methods: from microscopic...

10.1186/s12870-024-05226-x article EN cc-by BMC Plant Biology 2024-06-06

Several studies have shown beneficial effects of short exposure to oxidative stress on stored fruit, such as better preservation, increased firmness, preservation polyphenolic compounds, and reduced risk postharvest disorders bitter pit superficial scald in apples. In this study the effect short-term conditions physiology apple fruit was investigated. Apple three cultivars were exposed hypoxic storage various lengths induce anaerobiosis. The response evaluated by means cell wall...

10.1038/s41598-023-40782-6 article EN cc-by Scientific Reports 2023-08-24

The ultrastucture of mesophyll cells Deschampsia antarctica Desv. (Poaceae) leaves was investigated using the standard method preparing material for examination in transmission electron microscopy (TEM). were collected from Antarctic hairgrass growing a tundra microhabitat and representing xermorphic morphological anatomical features. general features are similar to those another grass leaves. observations ultrastructure have shown that organelles located close each other relatively small...

10.2478/v10067-012-0031-y article EN Annales Universitatis Mariae Curie-Sklodowska sectio C – Biologia 2013-01-01
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