A5033710140- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Viral-associated cancers and disorders
- Acute Myeloid Leukemia Research
- Multiple Myeloma Research and Treatments
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Virus-based gene therapy research
- Protein Degradation and Inhibitors
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Cytomegalovirus and herpesvirus research
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Immunodeficiency and Autoimmune Disorders
- CNS Lymphoma Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Hematological disorders and diagnostics
- DNA Repair Mechanisms
- Cancer Genomics and Diagnostics
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
University of Calgary
2016-2025
Calgary Laboratory Services
2013-2023
University of Alberta
2012-2022
Foothills Medical Centre
2010-2022
Indiana University – Purdue University Indianapolis
2020
University of Iowa Hospitals and Clinics
2020
Yale University
2020
The University of Texas MD Anderson Cancer Center
2000-2019
Medical College of Wisconsin
2019
Washington University in St. Louis
2019
Cellular pathways for induction of programmed cell death (PCD) have been identified, but little is known about specific extracellular matrix processes that may affect apoptosis along those pathways. In this study, a series Burkitt's lymphoma (BL) lines were assayed their expression tissue inhibitor metalloproteinases (TIMP)-1. Results indicate TIMP-1-positive BL show resistance to cold-shock-induced apoptosis. Furthermore, recombinant TIMP-1, not TIMP-2 or synthetic metalloproteinase...
MS4A4A is a member of the membrane‐spanning, four domain family, subfamily A (MS4A) that includes CD20 (MS4A1), FcRβ (MS4A2) and Htm4 (MS4A3). Like first three members this transcription appears to be limited hematopoietic cells. To evaluate expression protein in cell lineages subsets we generated monoclonal antibodies against extracellular epitopes for use flow cytometry. In human peripheral blood found expressed at plasma membrane monocytes but not granulocytes or lymphocytes. vitro...
B-cell non-Hodgkin lymphoma (B-NHL) encompasses multiple clinically and phenotypically distinct subtypes of malignancy with unique molecular etiologies. Common B-NHL, such as diffuse large lymphoma, have been comprehensively interrogated at the genomic level, but rarer subtypes, mantle cell remain less extensively characterized. Furthermore, B-NHL thus far not compared using same methodology to identify conserved or subtype-specific patterns alterations. Here, we employed a targeted...
We correlated bone marrow cytogenetic findings with morphologic and immunophenotypic data in 37 patients lymphoplasmacytic lymphoma (LPL)/Waldenström macroglobulinemia (WM). Each LPL/WM case was classified as lymphoplasmacytoid (n = 18), 10), or polymorphous 9) using the Kiel criteria. Of 12 cases chromosomal abnormalities, a single numeric abnormality present 4 complex karyotype 8. The most common abnormalities were -8 3 each; structural del(6q) 6 cases. Cytogenetic significantly less...
Of 92 patients with lymphoplasmacytic lymphoma/Waldenström macroglobulinemia (LPL/WM) treated at our institution, diffuse large B-cell lymphoma (DLBCL) also developed in 12 (13%). In 10 patients, DLBCL to 128 months (median, 44 months) after the diagnosis of LPL/WM. Two had LPL/WM and simultaneously. Clinicopathologic features did not predict risk DLBCL. Onset was characterized by worsening constitutional symptoms, profound cytopenias, extramedullary disease, organomegaly. Immunoglobulin...
Despite the recent advances made in treatment of multiple myeloma, disease still remains incurable. The oncolytic potential reovirus has previously been shown and is currently phase III clinical trials for solid tumors. We tested hypothesis that can successfully target human myeloma vitro, ex vivo, vivo without affecting hematopoietic stem cell (HHSC) re-population/differentiation a murine model partially recapitulates myeloma.Human lines tumor specimens were exposed to oncolysis mechanisms...
Graft-versus-host disease (GVHD) is a major transplantation complication. The purpose of this study was to measure immune cell subsets by flow cytometry early after (before median day GVHD onset) identify that may play role in pathogenesis. We also measured the later determine which be influenced or its treatment. studied 219 patients. found acute (aGVHD) preceded high counts CD4 T cells and CD8 cells. It followed low total naive B cells, cytolytic NK myeloid plasmacytoid dendritic Chronic...
Since 2014, programmed cell death protein 1 (PD-1)/programmed ligand (PD-L1) checkpoint inhibitors have been approved by various regulatory agencies for the treatment of multiple cancers including melanoma, lung cancer, urothelial carcinoma, renal head and neck classical Hodgkin lymphoma, colorectal gastroesophageal hepatocellular other solid tumors. Of these drug/disease combinations, a subset also has agency-approved, commercially available companion/complementary diagnostic assays that...
Abstract Objectives Recent studies show that blocking CD47-SIRPα interactions is a promising target in checkpoint inhibition for cancer immunotherapy. However, to date, the expression of CD47 not well characterized various hematolymphoid neoplasms. Methods This study evaluates wide range neoplasms using immunohistochemistry on 834 cases. Results variable but widespread among tumor types and within individual samples both intensity percentage. The highest expressions percentage positive...
Waldenstrom macroglobulinemia (WM) is a clinicopathologic syndrome in which B-cell neoplasm involving the bone marrow, usually lymphoplasmacytic lymphoma (LPL), associated with immunoglobulin M paraprotein serum. Extramedullary involvement occurs subset of patients and infrequently examined histologically. The files M.D. Anderson Cancer Center were searched for WM who underwent biopsy one or more extramedullary sites during course disease. Each specimen was classified using criteria World...
Multiple myeloma (MM) is a clonal plasma cell malignancy that accounts for 10-15% of newly diagnosed hematological cancers. Although significant advances have been made in the treatment MM disease still remains incurable. The oncolytic potential reovirus has previously demonstrated by others and us currently phase III clinical trials solid tumors. In addition I trial recently initiated MM. Despite activity, mechanism(s) death caused yet not well elucidated. A comprehensive understanding...
Leukocyte differentials are an important component of clinical care. Morphologic assessment peripheral blood smears (PBS) may be required to accurately classify leukocytes. However, manual microscopy is labor intensive. The CellaVision DM96 automated system that acquires digital images leukocytes on PBS, pre-classifies the cell type, and displays them screen for a Technologist or Pathologist approve reclassify. Our study compares results with microscopy.Three hundred fifty-nine PBS were...
The MS4A gene family in humans includes CD20 and at least 15 other genes. exists as homo-oligomers the plasma membrane, however different proteins expressed same cell may hetero-oligomerize. Given importance of B-cell function a therapeutic target, we sought to explore potential for hetero-oligomerization with proteins. We investigated expression primary human B-cells four genes previously shown be lines (MS4A4A, MS4A6A, MS4A7, MS4A8B), well two comprising closely related TMEM176 family,...
Abstract T cells engrafted with chimeric AgRs (CAR) are showing exciting potential for targeting B cell malignancies in early-phase clinical trials. To determine whether the second-generation CAR was essential optimal antitumor activity, two CD28-based constructs CD19 were tested their ability to redirect mouse function against established lymphoma a BALB/c syngeneic model system. armed either eliminated A20 vivo; however, one construct induced dose-dependent acute toxicity associated raised...
Summary. We present evidence that different B‐cell tumours, in bone marrow, have relationships to stroma. Marrow core biopsies from 46 patients with tumours were immunostained antibodies for distinct stromal cells. Cases included follicular lymphoma (FL), chronic lymphocytic leukaemia/small (CLL/SLL), mantle cell (MCL), lymphoplasmacytic (LPL), and nodal, extranodal splenic marginal zone (NMZL, MALT, SMZL). In normal low‐affinity nerve growth factor receptor (LNGFR) highlighted a fine...