A5023600314- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Plant Pathogenic Bacteria Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Disease Resistance and Genetics
- Forest Insect Ecology and Management
- Mycotoxins in Agriculture and Food
- Yeasts and Rust Fungi Studies
- Transgenic Plants and Applications
- Bacteriophages and microbial interactions
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Horticultural and Viticultural Research
- Botanical Research and Applications
- Essential Oils and Antimicrobial Activity
- Viral gastroenteritis research and epidemiology
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Agronomic Practices and Intercropping Systems
- Plant tissue culture and regeneration
- Plant pathogens and resistance mechanisms
- Probiotics and Fermented Foods
- Insect symbiosis and bacterial influences
University of Évora
2015-2025
Instituto Politécnico de Santarém
2023-2025
Research Center for Natural Resources, Environment and Society
2023-2025
Universidade Federal Rural de Pernambuco
2024
Universidade Federal da Paraíba
2024
Universidade Federal dos Vales do Jequitinhonha e Mucuri
2024
Universidade Estadual da Paraíba
2024
Universidade Federal de Lavras
2024
Consejo Superior de Investigaciones Científicas
2014
Fungal endophytes are micro-organisms that colonize healthy plant tissues without causing disease symptoms. They described as growth and resistance promoters have shown antimicrobial activity. The spatial-temporal distribution of endophytic communities in olive cultivars has been poorly explored. This study aims to investigate the richness diversity fungi different seasons sites, within Alentejo region, Portugal. Additionally, because impact some pathogenic (e.g. Colletotrichum spp.) varies...
Grapevine trunk diseases (GTDs) are the most widespread fungal diseases, affecting grapevines in all major growing regions of world, and their complete eradication is still not possible. Aiming to search alternatives avoid spread high incidence these present work intended molecularly identify grapevine endophytic community, phytopathogenic fungi associated with GTDs vineyards within Alentejo region, test potential antagonist microorganisms as biological control candidates against...
Grapevine trunk diseases (GTDs) are among the most important problems that affect longevity and productivity of vineyards in all major growing regions world. They slow-progression caused by several wood-inhabiting fungi with similar life cycles epidemiology. The simultaneous presence multiple pathogens a single plant together inconsistent GTDs symptoms expression, their isolation asymptomatic plants, absence effective treatments make these extremely complex to identify eradicate. Aiming gain...
Plant pathogens including viruses, bacteria, fungi, nematodes, and insects, can cause huge economic losses on a wide range of crops forest species worldwide [...]
Cactus pear var. miúda (Nopalea cochenillifera L. Salm-Dyck) is an important crop for the Northeast region of Brazil, composing one main sources animal feed. By April 2021, cladode rot caused death several cactus plants in a production area located Itaporanga, Paraíba state, Brazil (7°21'55.35" S and 38°11'38.68" W). The infected cladodes showed brown circular necrotic spots, soft with perforations that extended throughout cladode, followed by tipping over plants. incidence disease ranged...
Fusarium spp. and Magnaporthiopsis maydis are soil-inhabiting fungi respectively the causal agents of fusarium ear rot late wilt, two important diseases that can affect maize, one most cereal crops worldwide. Here, we present sensitive real-time PCR TaqMan MGB (Minor Groove Binder) assays detect discriminate several (F. oxysporum, F. verticillioides, graminearum) from M. maydis. The method is based on selective qPCR amplification internal transcribed spacer (ITS) region allows quantification...
In this study, the presence and variability of Colletotrichum spp. was evaluated by comparing fungal isolates obtained from olive trees under long-time phytosanitary treatments with without any (treated untreated, respectively). Olive fruits highly susceptible ‘Galega vulgar’ cultivar growing in Alentejo region were used as samples. From 210 sampled (half treated half untreated orchards), 125 (59.5%) presented spp., a significant lower number infected (39) when compared to orchards (86). The...
Venturia oleaginea and Pseudocercospora cladosporioides are two of the most important olive fungal pathogens causing leaf spots: peacock spot, cercosporiosis, respectively. In present study, communities associated with presence these were investigated. Overall, 300 symptomatic asymptomatic trees from different cultivars sampled Alentejo, Portugal. A total 788 isolates obtained classified into 21 OTUs; Ascomycota was clearly predominant phylum (96.6%). Trees cultivar 'Galega vulgar' showed a...
Tomato is one of the most important horticultural crops in world and severely affected by Fusarium diseases. To successfully manage these diseases, new insights on expression plant–pathogen interaction genes involved immunity responses to spp. infection are required. The aim this study was assess level field tomato samples evaluate differential target interactions groups presenting different levels. Our able detect 16 from a total 20 samples, proving effectiveness primer set designed ITS...
Olive anthracnose is a very common and severe disease caused by diverse species of fungi belonging to Colletotrichum acutatum gloeosporioides complexes. To understand aspects the colonization primary infection in olives, spp. were isolated from interior 2-year stems, flower buds, immature fruits three important olive cultivars, Galega vulgar, Cobrançosa, Azeiteira, different sites within Alentejo, major olive-producing region Portugal. A total 270 trees was sampled, 68 isolates obtained 46...
Sensitive detection of viruses in olive orchards is actually main importance since these pathogenic agents cannot be treated, their dissemination quite easy, and they can have eventual negative effects on oil quality. The work presented here describes the development application a new SYBR® Green-based real-time quantitative PCR (qPCR) analysis for specific reliable quantification highly spread tree viruses: Olive latent virus 1 (OLV-1), Tobacco necrosis D (TNV-D), mild mosaic (OMMV), leaf...
The genetic variability among 13 isolates of Olive mild mosaic virus (OMMV) and 11 Tobacco necrosis D (TNV-D) recovered from Olea europaea L. samples various sites in Portugal, was assessed through the analysis coat protein (CP) gene sequences. This amplified reverse transcriptase polymerase chain reaction (RT-PCR), cloned, 5 clone sequences each isolate, were analysed compared, including OMMV TNV-D originally different hosts countries available GenBank, totalling 131 encoded CP consisted...
Plant diseases result in severe losses to natural plant systems, and also cause problems for economics production agricultural systems [...]
Olea europaea Geminivirus (OEGV) was recently identified in olive Italy through HTS. In this work, we used HTS to show the presence of an OEGV isolate Portuguese trees and suggest evolution direction OEGV. The bipartite genome (DNA-A DNA-B) OEGV-PT is similar Old World begomoviruses length, but it lacks a pre-coat protein (AV2), which typical feature New (NW). DNA-A organization closer NW, containing four ORFs; three complementary-sense AC1/Rep, AC2/TrAP, AC3/REn one virion-sense AV1/CP, no...