A5005735997- Neonatal Respiratory Health Research
- Neuroscience of respiration and sleep
- Metabolism and Genetic Disorders
- Inhalation and Respiratory Drug Delivery
- Respiratory Support and Mechanisms
- Enzyme Structure and Function
- Biochemical Acid Research Studies
- Metal-Catalyzed Oxygenation Mechanisms
- Infant Nutrition and Health
- Maternal and Perinatal Health Interventions
- Porphyrin and Phthalocyanine Chemistry
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Protein Structure and Dynamics
- Biochemical and Molecular Research
- Cystic Fibrosis Research Advances
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Diet, Metabolism, and Disease
- Advanced Glycation End Products research
- Congenital Diaphragmatic Hernia Studies
- Cardiac Ischemia and Reperfusion
- Pancreatic function and diabetes
- Photosynthetic Processes and Mechanisms
- Clinical Nutrition and Gastroenterology
University of Nebraska at Kearney
2020-2025
Auburn University
2024
University of South Alabama
2024
Virginia Hospital Center
2018-2021
MedStar Washington Hospital Center
2018-2020
Michigan United
2018
MedStar Georgetown University Hospital
2018
Georgetown University
2006-2018
University of Michigan–Ann Arbor
2015-2018
University of Nebraska–Lincoln
2010-2017
Acute Respiratory Distress Syndrome (ARDS) is characterized by lung injury and damage to the alveolar type II cells. This study sought determine if endogenous surfactant altered in ARDS. Bronchoalveolar lavage was performed patients at-risk develop ARDS (AR, n = 20), with (A, 66) normal subjects (N, 29). The crude pellet analyzed for total phospholipids (PL), individual phospholipids, SP-A, SP-B, minimum surface tension (STmin). PL decreased both AR A (3.48 +/- 0.61 2.47 0.40 mumol/ml,...
Lung surfactant is deficient in patients with acute respiratory distress syndrome (ARDS). We performed a randomized, prospective, controlled, open-label clinical study of administration bovine to ARDS obtain preliminary information about its safety and efficacy. Patients received either by endotracheal instillation addition standard therapy or only. Three different groups receiving were studied: up eight doses 50 mg phospholipids/kg, those 100 four phospholipids/kg. Outcome measures included...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and biochemical characterization of CP4 (SP-D), a collagenous surfactant-associated proteinAnders Persson, Donald Chang, Kevin Rust, Michael Moxley, William Longmore, Edmond CrouchCite this: Biochemistry 1989, 28, 15, 6361–6367Publication Date (Print):July 25, 1989Publication History Published online1 May 2002Published inissue 25 July 1989https://pubs.acs.org/doi/10.1021/bi00441a031https://doi.org/10.1021/bi00441a031research-articleACS...
Objective. To evaluate components of pulmonary surfactant and identify mutations in the protein B gene (SP-B) a term infant with severe respiratory distress chronic lung disease. Patient Testing. Respiratory developed an delivered at term, he required extracorporeal bypass support for 2 weeks. Until his unexpected death 9.5 months, was ventilator oxygen dependent continual dexamethasone therapy. Tracheobronchial lavage samples were analyzed content proteins (SPs), DNA from blood sequenced by...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCP4: a pneumocyte-derived collagenous surfactant-associated protein. Evidence for heterogeneity of surfactant proteinsAnders Persson, Kevin Rust, Donald Chang, Michael Moxley, William Longmore, and Edmound CrouchCite this: Biochemistry 1988, 27, 23, 8576–8584Publication Date (Print):November 15, 1988Publication History Published online1 May 2002Published inissue 15 November...
Abstract Human DJ‐1 is a cytoprotective protein whose absence causes Parkinson's disease and also associated with other diseases. has an established role as redox‐regulated that defends against oxidative stress mitochondrial dysfunction. Multiple studies have suggested protein/nucleic acid deglycase plays key in the repair of glycation damage caused by methylglyoxal (MG), reactive α‐keto aldehyde formed central metabolism. Contradictory reports suggest glyoxalase but not does play major...
Surface-active phospholipid-containing particles are traditionally considered to be the product of type II pneumocytes. We now demonstrate membrane-bound lamellar cytoplasmic organelles in adult and suckling rat enterocytes that densely reactive with phospholipid-staining reagents. These structures were seen basolateral space, within intercellular junctions, unraveling on lumenal surface, more abundant after fat feeding. Light scrapings intestinal mucosa washings contained these bodies, as...
Exposure of insulin-producing cells to elevated levels the free fatty acid (FFA) palmitate results in loss β-cell function and induction apoptosis. The endoplasmic reticulum (ER) stress is one mechanism proposed be responsible for viability response treatment; however, pathways ER by have yet determined. Protein palmitoylation a major posttranslational modification that regulates protein localization, stability, activity. Defects in, or dysregulation of, could which may induce β-cells....
A complete understanding of enzyme mechanisms requires atomistic details chemical reactions. Quantum-based molecular dynamics simulations (QMD) are a potential source this information, but tradeoffs between accuracy and computational cost have limited their use. Here, we develop reactive QMD approach to investigate isocyanide hydratase (ICH) catalysis. In simulations, analogs ICH active site residues reacted with para-nitrophenol isocyanide, forming thioimidate intermediate. Analysis...
A complete understanding of enzyme mechanisms requires atomistic details chemical reactions. Quantum-based molecular dynamics simulations (QMD) are a potential source this information, but tradeoffs between accuracy and computational cost have limited their use. Here, we develop reactive QMD approach to investigate isocyanide hydratase (ICH) catalysis. In simulations, analogs ICH active site residues reacted with para-nitrophenol isocyanide, forming thioimidate intermediate. Analysis...
A complete understanding of enzyme mechanisms requires atomistic details chemical reactions. Quantum-based molecular dynamics simulations (QMD) are a potential source this information, but tradeoffs between accuracy and computational cost have limited their use. Here, we develop reactive QMD approach to investigate isocyanide hydratase (ICH) catalysis. In simulations, analogs ICH active site residues reacted with para-nitrophenol isocyanide, forming thioimidate intermediate. Analysis...
A complete understanding of enzyme mechanisms requires atomistic details chemical reactions. Quantum-based molecular dynamics simulations (QMD) are a potential source this information, but tradeoffs between accuracy and computational cost have limited their use. Here, we develop reactive QMD approach to investigate isocyanide hydratase (ICH) catalysis. In simulations, analogs ICH active site residues reacted with para-nitrophenol isocyanide, forming thioimidate intermediate. Analysis...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTThe major glycolipid recognized by SP-D in surfactant is phosphatidylinositolAnders V. Persson, Brian J. Gibbons, James D. Shoemaker, Michael A. Moxley, and William LongmoreCite this: Biochemistry 1992, 31, 48, 12183–12189Publication Date (Print):December 8, 1992Publication History Published online1 May 2002Published inissue 8 December 1992https://pubs.acs.org/doi/10.1021/bi00163a030https://doi.org/10.1021/bi00163a030research-articleACS...
The 5′ flanking region of the Clara cell secretory protein (CCSP) gene contains two cis-acting elements which bind hepatocyte nuclear factor (HNF)-3 alpha and HNF-3 beta in vitro. To determine role these proteins mediating CCSP expression bronchiolar epithelium, chimeric CCSP-reporter constructs containing various regions were co-transfected into H-441 cells with or plasmids. These studies indicate that each transcription factors positively regulates revealed I (-132 to -76) is sufficient...
Section:ChooseTop of pageAbstract <<Materials and MethodsResultsDiscussionReferencesCITING ARTICLES
Pulmonary surfactant replacement has previously been shown to be effective in the human neonatal respiratory distress syndrome. The value of models acute lung injury other than quantitative deficiency states is, however, uncertain. In this study an model using rats with chronic indwelling arterial catheters, injured N-nitroso-N-methylurethane (NNNMU), developed. NNNMU was found produce hypoxia, increased mortality, alveolitis, and alterations pulmonary system. Alterations obtained by...