Botrytis cinerea Pers. Fr. is an important pathogen causing stem rot in tomatoes grown indoors for extended periods. MicroRNAs (miRNAs) have been reported as gene expression regulators related to several stress responses and B. infection tomato. However, the function of miRNAs resistance remains unclear. The miRNA patterns tomato response were investigated by high-throughput sequencing. In total, 143 known seven novel identified their corresponding was detected mock- cinerea-inoculated...

Long non-coding RNAs (lncRNAs) represent a class of riboregulators that either directly act in long form or are processed to shorter miRNAs and siRNAs. Emerging evidence shows lncRNAs participate stress responsive regulation. In this study, identify the putative maize drought stress, 8449 transcripts were first uploaded Coding Potential Calculator website for classification as protein coding RNAs, 1724 identified potential RNAs. A Perl script was written screen these ncRNAs 664 ultimately...

Post-translational modification of proteins through lysine succinylation plays important regulatory roles in living cells. Lysine was recently identified as a novel post-translational Escherichia coli, yeast, Toxoplasma gondii, HeLa cells, and mouse liver. Interestingly, only few sites have been detected plants to date. In this study, we 347 202 tomato by using high-resolution mass spectrometry. Succinylated are implicated the regulation diverse metabolic processes, including chloroplast...

Abstract Summary: Emerging evidence has revealed phased siRNAs (phasiRNAs) as important endogenous regulators in plants. However, the integrated prediction tools for phasiRNAs are still limited. In this article, we introduce a stand-alone package PhaseTank systematically characterizing and their regulatory networks. (i) It can identify phasiRNAs/tasiRNAs functional cascades (miRNA/phasiRNA→ PHAS loci→phasiRNA→target) with high sensitivity specificity. (ii) By one command analysis, it...

Northern leaf blight, caused by the fungus Exserohilum turcicum (Pass.), is a major disease of maize (Zea mays L.). MicroRNAs (miRNAs) have been recently reported as gene expression regulators related to several stress responses; however, evidence role miRNAs in plant response biotic stresses limited. In this study, miRNA patterns E. were investigated using microarray platform. A total 118 detected mock- and turcicum-inoculated leaves. Among these miRNAs, miR530, miR811, miR829, miR845...

Along with the well-studied microRNA (miRNA) and small interfering RNA (siRNA) is a new class of transfer RNA-derived (tsRNA), which has recently been detected in multiple organisms are implicated gene regulation. However, while miRNAs siRNAs known to repress expression through sequence-specific cleavage or translational repression, how tsRNAs regulate remains unclear. Here we report identification functional characterization oomycete pathogen Phytophthora sojae. We show that tRNAs processed...

Recent evidence shows that small RNAs are transferred from a species to another through cross-species transmission and exhibit biological activities in the receptor. In this study, we focused on tomato-derived sRNAs play role of defense against Botrytis cinerea. Bioinformatics method was firstly employed identify tomato-encoded as antifungal factors targeting B. cinerea genes. Then expression levels some identifed were checked cinerea-infected plant using qRT-PCR method. Exogenic RNA-induced...

Abstract The development of RNA interference (RNAi) is crucial for studying plant gene function. Its use, limited to a few plants with well‐established transgenic techniques. Spray‐induced silencing (SIGS) introduces exogenous double‐stranded (dsRNA) into by spraying, injection, or irrigation, triggering the RNAi pathway instantly silence target genes. As transient technology that does not rely on methods, SIGS has significant potential function in lacking advanced technology. In this study,...

Salvia miltiorrhiza has been comprehensively studied as a medicinal model plant. However, research progress on this species is significantly hindered by its unavailable genome sequences and limited number of expressed sequence tags in the National Center for Biotechnology Information database. Thus, transcript database must be developed to assist researchers browse, search, align gene cloning functional analysis S. miltiorrhiza. In study, Danshen Transcriptional Resource Database (DsTRD) was...

Gray mold of tomato is caused by the pathogen Botrytis cinerea. MicroRNAs play a crucial role in biotic and abiotic stress responses plants regulate their targets gene silencing. MiR394 an ancient conserved miRNA plants, it participates regulation plant development responses. In our previous study, miR394 was found to respond B. cinerea infection tomato, but roles regulatory mechanisms cinerea-infected remain unclear. down-regulated response infection, showing expression pattern opposite...

Although domesticated tomato is cultivated by wild tomato, there are a lot of differences between and such as shape, physiological function life history. Many studies show that has better salt resistance drought resistance. In addition to, tomato's fruit bigger more nutritious than tomato. The different features closely related to differentially expressed genes. We identified 126 up-regulated genes 87 down-regulated in RNA-Seq. These may be associated with resistance, nutrition. also further...

Replanting disease is a major factor limiting the artificial cultivation of traditional Chinese medicinal herb Salvia miltiorrhiza. At present, little information available regarding role miRNAs in response to replanting disease. In this study, two small RNA libraries obtained from first-year (FPR) and second-year plant (SPR) roots were subjected high-throughput sequencing method. Bioinformatics analysis revealed that 110 known 7 novel annotated S. Moreover, 39 2 identified validated for...

Coronavirus infectious disease 2019 (COVID-19), caused by severe acute respiratory virus type 2 (SARS-CoV-2), has a global public health crisis. As an RNA virus, the high gene mutability of SARS-CoV-2 poses significant challenges to development broad-spectrum vaccines and antiviral therapeutics. There remains lack specific therapeutics directly targeting SARS-CoV-2. With ability efficiently inhibit expression target genes in sequence-specific way, small interfering (siRNA) therapy exhibited...

Spray-induced gene silencing represents an eco-friendly approach for crop protection through the use of double-stranded RNA (dsRNA) to activate interference (RNAi) pathway, thereby crucial genes in pathogens. The major challenges associated with dsRNA are its limited stability and poor cellular uptake, necessitating repeated applications effective protection. In this study, nanoparticles (NPs) were proposed as effectors plants pathogens by inducing RNAi pathway expression. structural motifs,...

This study explores the use of Generative Artificial Intelligence (GAI) in assessing pilot fatigue risk by integrating facial recognition and physiological signals with Inertial Measurement Units (IMUs). By leveraging IMU technology's precise, real-time data on movement combining it GAI's advanced analysis capabilities, aims to enhance accuracy prediction models. The reveals that while traditional classifiers like Extreme Random Trees Forests offer modest performance, models such as Support...