A5087939494- Lanthanide and Transition Metal Complexes
- Advanced MRI Techniques and Applications
- MRI in cancer diagnosis
- Acute Kidney Injury Research
- Electrolyte and hormonal disorders
- Renal and related cancers
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- Chronic Kidney Disease and Diabetes
- Nitric Oxide and Endothelin Effects
- Radiation Dose and Imaging
- Muscle and Compartmental Disorders
- SARS-CoV-2 and COVID-19 Research
- Renal cell carcinoma treatment
- Amino Acid Enzymes and Metabolism
- Renal function and acid-base balance
- Renal and Vascular Pathologies
- Sulfur Compounds in Biology
- Radioactive element chemistry and processing
- Intracranial Aneurysms: Treatment and Complications
- Tissue Engineering and Regenerative Medicine
- Diabetes and associated disorders
- Vaccine Coverage and Hesitancy
- COVID-19 epidemiological studies
- Pregnancy and preeclampsia studies
New Mexico VA Health Care System
2018-2025
University of New Mexico
2018-2025
New Mexico Heart Institute
2019-2024
Raymond G. Murphy VA Medical Center
2021-2024
University of New Mexico Hospital
2018
The University of Texas Health Science Center at San Antonio
2001-2017
South Texas Veterans Health Care System
2003-2017
Murphy Oil Corporation (United States)
2001-2017
Philadelphia College of Osteopathic Medicine
2010
Universität Hamburg
2008
Renal hypertrophy and extracellular matrix accumulation are early features of diabetic nephropathy. We investigated the role NAD(P)H oxidase Nox4 in generation reactive oxygen species (ROS), hypertrophy, fibronectin expression a rat model type 1 diabetes induced by streptozotocin. Phosphorothioated antisense (AS) or sense oligonucleotides for were administered 2 weeks with an osmotic minipump 72 h after streptozotocin treatment. protein was increased kidney cortex compared non-diabetic...
Infections by viruses are associated with approximately 12% of human cancer. Kaposi’s sarcoma-associated herpesvirus (KSHV) is causally linked to several malignancies commonly found in AIDS patients. The mechanism KSHV-induced oncogenesis remains elusive, due part the lack an adequate experimental system for cellular transformation primary cells. Here, we report efficient infection and rat embryonic metanephric mesenchymal precursor cells (MM cells) KSHV. Cellular occurred at as early day 4...
Angiotensin II (Ang II) stimulates hypertrophy of glomerular mesangial cells. The signalling mechanism by which Ang exerts this effect is not precisely known. Downstream potential targets are the extracellular-signal-regulated kinases 1 and 2 (ERK1/ERK2). We demonstrate that activates ERK1/ERK2 via AT1 receptor. Arachidonic acid (AA) mimics action on phospholipase A2 inhibitors blocked II-induced activation. antioxidant N-acetylcysteine as well NAD(P)H oxidase diphenylene iodonium...
In hyperglycemia, the serum sodium concentration ([Na]S) receives influences from (a) fluid exit intracellular compartment and thirst, which cause [Na]S decreases; (b) osmotic diuresis with sums of urinary plus potassium lower than baseline euglycemic [Na]S, results in a increase; (c), some cases, gains or losses fluid, sodium, through gastrointestinal tract, respiratory skin. Hyperglycemic patients hypernatremia have large deficits body water usually hypovolemia develop severe clinical...
It has been presupposed that the thermodynamic stability constant (K(therm)) of gadolinium-based MRI chelates relate to risk precipitating nephrogenic systemic fibrosis. The present study compared low-K(therm) gadodiamide with high-K(therm) gadoteridol in cultured fibroblasts and rats uninephrectomies. Gadolinium content was assessed using scanning electron microscopy equipped energy-dispersive X-ray spectroscopy paraffin-embedded tissues. In vitro, demonstrated dose-dependent fibronectin...
Colony-stimulating factor-1 (CSF-1), released by osteoblasts, stimulates the proliferation of osteoclast progenitors via c-fms receptor (CSF-1R) and, in combination with activator nuclear factor-kappaB ligand (RANKL), leads to formation mature osteoclasts. Whether CSF-1R is expressed osteoblasts and mediates specific biological effects has not been explored. Wild-type primary calvaria (OB) were analyzed for expression (RT-PCR Western blot) functionality (immunocomplex kinase assay). OB serum...
Abstract The leitmotifs of magnetic resonance imaging (MRI) contrast agent-induced complications range from acute kidney injury, symptoms associated with gadolinium exposure (SAGE)/gadolinium deposition disease, potentially fatal encephalopathy, and irreversible systemic fibrosis. Gadolinium is the active ingredient these agents, a non-physiologic lanthanide metal. mechanisms MRI diseases are unknown. Mice were treated agent. Human tissues contrast-naïve agent-treated patients obtained...
Mice deficient in either platelet-derived growth factor (PDGF) B chain or PDGF receptor (PDGFR) β lack mesangial cells. stimulates proliferation and migration of metanephric mesenchymal cells, from which cells are derived. Binding to PDGFR-β induces autophosphorylation at specific tyrosine residues activates various effector proteins, including phosphatidylinositol-3-kinase (PI3-K). This study explored the role PI 3-K reactive oxygen species (ROS) PDGF-mediated signaling using established...
Gadolinium-based contrast agents are increasingly used in clinical practice. While these pharmaceuticals verified causal nephrogenic systemic fibrosis, there is a growing body of literature supporting their role as symptoms associated with gadolinium exposure after intravenous use and encephalopathy following intrathecal administration. multidentate organic ligands that strongly bind the metal ion to reduce toxicity metal. The notion cationic dissociates from chelates causes disease...
Systemic fibrosis can be induced in humans with gadolinium-based contrast, and cumulative doses correlate severity. Bone marrow–derived fibrocytes accumulate the dermis. Whether target organs liberate chemokines to recruit these or whether are stimulated home affected tissue is unknown. Transgenic (tagged) donor rats were treated contrast. marrow was obtained from diseased animals age-matched controls. Rats subtotal nephrectomies lethally irradiated underwent salvage transplantation either...
PDGF B chain or receptor (PDGFR)-β-deficient (−/−) mice lack mesangial cells. To study responses of α- and β-receptor activation to ligands, metanephric mesenchymal cells (MMCs) were established from embryonic day E11.5 wild-type (+/+) −/− mouse embryos. BB stimulated cell migration in +/+ cells, whereas AA did not. Conversely, was chemotactic for MMCs. The mechanism by which PDGFR-β inhibited AA-induced investigated. BB, but not AA, increased intracellular Ca 2+ the production reactive...
The adult kidney is derived from the interaction between metanephric blastema and ureteric bud. Platelet-derived growth factor (PDGF) receptor β essential for development of mature glomerular tuft, as mice deficient this lack mesangial cells. This study investigated role Src tyrosine kinase in PDGF-mediated reactive oxygen species (ROS) generation migration mesenchymal cells (MMCs). Cultured embryonic MMCs wild-type PDGF receptor-deficient embryos were established. Migration was determined...
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Hypertonic saline infusion is used to correct hyponatremia with severe symptoms. The selection of the volume infused hypertonic ( V Inf ) should address prevention overcorrection or undercorrection. Several formulas computing this have been proposed. limitations common these consist (1) failure include potential determinants change in serum sodium concentration ([ Na ]) including exchanges between osmotically active and inactive compartments, changes hydrogen binding body water hydrophilic...