A5069511584- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- DNA and Nucleic Acid Chemistry
- Advancements in Solid Oxide Fuel Cells
- Fuel Cells and Related Materials
- Electrocatalysts for Energy Conversion
- Nanoparticle-Based Drug Delivery
- Molecular Biology Techniques and Applications
- Polymer Surface Interaction Studies
- Central Venous Catheters and Hemodialysis
- Intestinal and Peritoneal Adhesions
- Catalytic Processes in Materials Science
- Chemical Looping and Thermochemical Processes
- Mechanical Circulatory Support Devices
- Nanoplatforms for cancer theranostics
- Catalysis and Hydrodesulfurization Studies
- Gas Sensing Nanomaterials and Sensors
- Photoacoustic and Ultrasonic Imaging
- Cardiac Arrest and Resuscitation
- Thermography and Photoacoustic Techniques
- Vitamin D Research Studies
- Vitamin C and Antioxidants Research
Prefectural University of Hiroshima
2024
Showa University Koto Toyosu Hospital
2018
Showa University
2018
The University of Tokyo
2014-2017
Graduate School USA
2015
Kobe University
2011-2014
Utsunomiya University
1992
A critical role of polyethylene glycol (PEG) crowding in the packaging plasmid DNA (pDNA) into polyplex micelles (PMs) was investigated using a series PEG-b-poly(l-lysine) (PEG–PLys) block copolymers with varying molecular weights both PEG and PLys segments. Rod-shaped PMs preferentially formed when tethered chains covering pDNA precondensed state were dense enough to overlap one another (reduced tethering density (RTD) > 1), whereas globular obtained they not overlapped (RTD < 1). These...
The role of poly(ethylene‐glycol) (PEG) in rod‐shaped polyplex micelle structures, having a characteristic core folded plasmid DNA (pDNA) and shell tethered PEG chains, is investigated using PEG‐detachable micelles. Rod shapes undergo change to compacted globule by removal from micelles prepared block copolymer with acid‐labile linkage between poly( l ‐lysine) (PLys) through exposure acidic milieu. This structural supports the previous investigation on rod that prevents structure being...
The applicability of small interfering RNA (siRNA) in future therapies depends on the availability safe and efficient carrier systems. Ideally, siRNA delivery requires a system that is stable circulation but upon specific uptake into target cells can rapidly release its cargo cytoplasm. Previously, we evaluated novel generation systems ("decationized" polyplexes) for DNA delivery, it was shown folate targeted decationized polyplexes had an excellent safety profile showed intracellular...
Selectively spooling single plasmid DNA (pDNA), as a giant polyelectrolyte, into nanosized toroidal structure or folding it rod-like has been accomplished by polyion complexation with block catiomers to form polymeric micelles in varying NaCl concentrations. The interactive potency between the pDNA and was determined play critical role defining ultimate of pDNA; formation structures achieved 600 0 mM solutions, respectively. Compared structure, possessed superior biological functions capable...
We present the cases of eight patients (mean age 75 years; EuroSCORE II 17.0 ± 22.0) who underwent post-cardiotomy venovenous extracorporeal membrane oxygenation (ECMO) without heparinization due to serious bleeding. Three liver cirrhosis, two chronic hemodialysis, three redo sternotomy, and urgent surgery were included. Respiratory ECMO Survival Prediction score was - 5.1 4.2 (estimated survival rate: approximately 30%). Mean duration 14 days with 9 circuit exchanges. Five weaned from...
A tadpole-shaped nanostructure characterized by phase segregation occurring in a single pDNA-containing polymeric micelle is shown.
Upon PEG removal, the change of rod shapes into globule is observed for packaged DNA in polyplex micelles that are prepared from block copolymers with acid-labile linkage between and poly(L-lysine) (PLys). On page 1193, K. Osada, Kataoka, co-workers use details this transition behavior to elucidate essential regulating factors micelle structures, order find their structure–function relationship promote utilization as a gene delivery system.
High temperature Raman spectroscopy of Sm doped CeO 2 /carbonate composite was measured in order to discuss the interaction between carbonate ion and solid oxide electrolyte at an interface LT-SOFC. In spectra with breathing mode NK initial heating process, whereas separated ν 1 (CO 3 2- ) bands assigned Na CO K are observed clearly lower temperature, unifying these due state migration carbonates than eutectic point (983 K). Degree is affected by apparent average thickness (AAT) amount...
In pancreatic cancer, the access of therapeutic reagents to cancer cells was restricted by thick fibrotic tissue, and thus anti-angiogenic therapy targeting endothelial cells, which are more accessible, is a promising strategy. Gene based on messenger RNA (mRNA) delivery allows for sustainable introduction factors without risks associated with DNA delivery, such as insertion mutagenesis, uncontrollably prolonged transgene expression. Systemic intravenous injection proper route mRNA from...
It is well-known that high temperature fuel cells as MCFCs and SOFCs operated at ca. 923-1273 K,generate electricity with power density efficiency. Since component materials cell configuration sustainable operating under oxidation/ reduction atmosphere are undefined, comprehensive improvement for included electrodes electrolyte needed. Composite including solid oxide carbonate indicate conductivity relative low expected various can be utilized the operation of etc. Wang et al. consider there...
Abstract not Available.
DC etching of aluminum is used in the fabrication electrodes to enlarge surface area base plate, thereby increasing its capacitance. To clarify basic mechanism this process, situ monitoring was attempted using a photoacoustic (PA) technique. The plate 99.99% foil, electrolyte an aqueous solution 2mol/dm3 HCl, 0.2mol/dm3 AlCl3. Etching done under galvanostatic conditions at 80°C. light source for PA technique Ar ion laser beam that chopped mechanically. A signal from piezoelectric transducer...