A5057494396- Per- and polyfluoroalkyl substances research
- Toxic Organic Pollutants Impact
- Effects and risks of endocrine disrupting chemicals
- Connective tissue disorders research
- Air Quality and Health Impacts
- Animal testing and alternatives
- Protease and Inhibitor Mechanisms
- Birth, Development, and Health
- Retinal and Macular Surgery
- Environmental Toxicology and Ecotoxicology
- Proteoglycans and glycosaminoglycans research
- Health, Environment, Cognitive Aging
- Reproductive Biology and Fertility
- Luminescence and Fluorescent Materials
- Heat shock proteins research
- Fluoride Effects and Removal
- Aortic aneurysm repair treatments
- Atmospheric chemistry and aerosols
- Physiological and biochemical adaptations
- Anesthesia and Neurotoxicity Research
- Corneal surgery and disorders
- Microfluidic and Bio-sensing Technologies
- Single-cell and spatial transcriptomics
- Polysaccharides and Plant Cell Walls
- Adipose Tissue and Metabolism
University of Alberta
2010-2024
Health Canada
2020-2024
Carleton University
2013
Manchester Royal Eye Hospital
2000
Wellcome Centre for Cell-Matrix Research
1998-2000
University of Manchester
1994-2000
The bioaccumulation of perfluoroalkylated substances (PFASs) in plankton has previously been evaluated only freshwater and regional seas, but not for the large oligotrophic global oceans. Plankton samples from tropical subtropical Pacific, Atlantic Indian Oceans were collected during Malaspina 2010 circumnavigation expedition, analyzed 14 ionizable PFASs, including perfluorooctanoate (PFOA), perfluorooctanesulfonate (PFOS) their respective linear branched isomers. PFOA PFOS concentrations...
Abstract Per- and poly-fluoroalkyl substances (PFAS) are widely found in the environment because of their extensive use persistence. Although several PFAS well studied, most lack toxicity data to inform human health hazard risk assessment. This study focused on 4 model PFAS: perfluorooctanoic acid (PFOA; 8 carbon), perfluorobutane sulfonate (PFBS; perfluorooctane (PFOS; perfluorodecane (PFDS; 10 carbon). Human primary liver cell spheroids (pooled from donors) were exposed concentrations each...
Use of molecular data in human and ecological health risk assessments industrial chemicals agrochemicals has been anticipated by the scientific community for many years; however, these are rarely used assessment. Here, a logic framework is proposed to explore feasibility future development transcriptomic methods refine replace current apical endpoint-based regulatory toxicity testing paradigm. Four foundational principles outlined discussed that would need be accepted stakeholders prior this...
The growing number of chemicals in the current consumer and industrial markets presents a major challenge for regulatory programs faced with need to assess potential risks they pose human ecological health. increasing demand hazard risk assessment currently exceeds capacity produce toxicity data necessary decision making, applied is commonly generated using traditional approaches animal models that have limited context terms relevance. This scenario provides opportunity implement novel, more...
Per- and polyfluoroalkyl substances (PFAS) are some of the most prominent organic contaminants in human blood. Although toxicological implications exposure to perfluorooctane sulfonate (PFOS) perfluorooctanoate (PFOA) well established, data on lesser-understood PFAS limited. New approach methodologies (NAMs) that apply bioinformatic tools high-throughput being increasingly considered inform risk assessment for data-poor chemicals. The aim this study was compare potencies (ie, benchmark...
Per- and polyfluoroalkyl substances (PFAS) are a wide range of chemicals that used in variety consumer industrial products leading to direct human exposure. Many PFAS chemically nonreactive persistent the environment, resulting additional exposure from water, soil, dietary intake. While some have documented negative health effects, data on simultaneous exposures multiple (PFAS mixtures) inadequate for making informed decisions risk assessment. The current study leverages previous work our...
Serum perfluoroalkyl acids (PFAAs) have been linked to disruption of maternal thyroid hormone homeostasis, but results varied between studies which we hypothesized was due timing the measurements, variability in PFAA isomer patterns, or presence other stressors. In a longitudinal study design, investigated time-dependency associations isomers and hormones during pregnancy post-partum while considering peroxidase antibody (TPOAb) status mercury (Hg) co-exposure. participants prospective...
Since initial regulatory action in 2010 Canada, bisphenol A (BPA) has been progressively replaced by structurally related alternative chemicals. Unfortunately, many of these chemicals are data-poor, limiting toxicological risk assessment. We used high-throughput transcriptomics to evaluate potential hazards and compare potencies BPA 15 cultured breast cancer cells. MCF-7 cells were exposed (0.0005-100 µM) for 48 h. TempO-Seq (BioSpyder Inc) was examine global transcriptomic changes estrogen...
Perfluoroalkyl acids (PFAAs) within the broader class of per- and polyfluoroalkyl substances (PFAS) are present in human serum as isomer mixtures, but epidemiological studies have yet to address isomer-specific associations with child development behavior.To examine between prenatal exposure 25 PFAAs, including perfluorooctane sulfonate (PFOS) perfluorooctanoate (PFOA) isomers, neurodevelopment among 490 mother-child pairs a prospective Canadian birth cohort, Alberta Pregnancy Outcomes...
The increasing global prevalence of gestational diabetes mellitus (GDM) has been hypothesized to be associated with maternal exposure environmental chemicals. Here, among 420 women participating in the Alberta Pregnancy Outcomes and Nutrition (APrON) cohort study, we examined associations between GDM second trimester blood or urine concentrations endocrine disrupting chemicals (EDCs): bisphenol-A (BPA), bisphenol-S (BPS), twelve phthalate metabolites, eight perfluoroalkyl acids (PFAAs),...
Methylmercury (MeHg) and perfluorooctanesulfonate (PFOS) are major contaminants of human blood that both common in dietary fish, thereby raising questions about their combined impact on development. Here, pregnant Sprague-Dawley rats ingested a daily dose, from gestational day 1 through to weaning, either mg/kg bw PFOS (PFOS-only), MeHg (MeHg-only), mixture 0.1 (Low-Mix), or (High-Mix). Newborns were monitored for physical milestones reflexive developmental responses, juveniles the...
Flow cytometry is a key instrument in biological studies, used to identify and analyze cells suspension. The identification of from debris commonly based on light scatter properties as it has been shown that there relationship between forward scattered cell volume this become common practice flow cytometry. Cryobiological conditions induce changes alter their properties. Cells with membrane damage freeze–thaw stress produce lower signals may fall below standard thresholds. In contrast cannot...
The success of cryopreservation protocols is largely based on membrane integrity assessments after thawing, since can be considered to give an upper limit in assessment cell viability and the plasma a primary site cryoinjury. However, exposure cells conditions associated with low temperatures induce injury cellular structure function that may not readily identified by alone. Interrupted cooling (including interrupted slow without hold time (graded freezing), rapid (two-step freezing)), yield...
Maternal exposure to per- and polyfluoroalkyl substances (PFAS) has been linked child neurodevelopmental difficulties. Neuroimaging research these difficulties white matter microstructure alterations, but the effects of PFAS on children's remains unclear. We investigated associations between maternal blood concentrations six common perfluoroalkyl sulfonates alterations in young children using longitudinal neuroimaging data. This study included 84 maternal-child pairs from a Canadian...
Abstract Per- and polyfluoroalkyl substances (PFAS) are some of the most prominent organic contaminants in human blood. Although toxicological implications from exposure to perfluorooctane sulfonate (PFOS) perfluorooctanoate (PFOA) well established, data on lesser-understood PFAS limited. New approach methodologies (NAMs) that apply bioinformatic tools high-throughput being increasingly considered inform risk assessment for data-poor chemicals. The aim this investigation was identify...
Abstract Per- and poly-fluoroalkyl substances (PFAS) are widely found in the environment because of their extensive use persistence. Although several PFAS well studied, most lack toxicity data to inform human health hazard risk assessment. This study focussed on four model PFAS: perfluorooctanoic acid (PFOA; 8 carbon), perfluorobutane sulfonate (PFBS; 4 perfluorooctane (PFOS; perfluorodecane (PFDS; 10 carbon). Human primary liver cell spheroids (pooled from donors) were exposed...