A5086689278- Wound Healing and Treatments
- Dermatologic Treatments and Research
- Bacterial biofilms and quorum sensing
- Diabetic Foot Ulcer Assessment and Management
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Electrospun Nanofibers in Biomedical Applications
- Dermatology and Skin Diseases
- Nuclear Receptors and Signaling
- Antimicrobial Resistance in Staphylococcus
- Pressure Ulcer Prevention and Management
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Antimicrobial Peptides and Activities
- Organ and Tissue Transplantation Research
- S100 Proteins and Annexins
- Surgical Sutures and Adhesives
- Ion Channels and Receptors
- Immune Response and Inflammation
- Cancer-related gene regulation
- Biomedical Ethics and Regulation
- Reconstructive Surgery and Microvascular Techniques
- Ion channel regulation and function
- Periodontal Regeneration and Treatments
- Laser Applications in Dentistry and Medicine
Northwestern University
2015-2024
University of North Carolina at Chapel Hill
2024
University of Chicago
2020
Université de Montréal
2020
Plastic Surgery Hospital
2012-2013
Plastic Surgery Foundation
2013
McLean Hospital
2000-2010
Harvard University
2000-2010
Harvard University Press
2005
Laboratory of Molecular Genetics
2003
Abstract Tyrosine hydroxylase (TH) catalyzes the first and rate‐limiting step of catecholamine synthesis its expression is necessary for neurotransmitter specification all catecholaminergic neurons, while dopamine β‐hydroxylase (DBH) essential noradrenergic phenotype. In present study, we show that Nurr1, an orphan nuclear receptor critical dopaminergic (DA) neuron development, directly transactivates promoter activity TH gene in a cell type‐dependent manner, it does not regulate DBH...
A growing body of evidence suggests that in addition to hypoxia, ischemia-reperfusion injury, and intrinsic host factors, bacterial biofilms represent a fourth major pillar chronic wound pathogenesis. Given most studies date rely on vitro or observational clinical data, our aim was develop novel, quantitative animal model enabling further investigation the biofilm hypothesis vivo. Dermal punch wounds were created New Zealand rabbit ears, used as uninfected controls, inoculated with green...
Multipotent mesenchymal stem cells (MSCs) are found in various tissues and can proliferate extensively vitro. MSCs have been used preclinical animal studies clinical trials many fields. Adipose derived (ASCs) several advantages compared to other for use cell-based treatments because they easy isolate with relative abundance. However, quantitative approaches wound repair using ASCs limited of lack models which allow quantification. Here, we addressed the effect topically delivered by analysis...
Bacterial biofilms, which are critical mediators of chronic wounds, remain difficult to treat with traditional methods. Bacteriophage therapy against biofilm has not been rigorously studied in vivo. The authors evaluate the efficacy a species-specific bacteriophage Staphylococcus aureus biofilm-infected wounds using validated, quantitative, rabbit ear model.Six-millimeter dermal punch New Zealand ears were inoculated wild-type or mutant, biofilm-deficient S. aureus. In vivo was established...
Heterozygous mutations of the PHOX2B gene account for a broad variety disorders autonomic nervous system, either isolated or combined, including congenital central hypoventilation syndrome (CCHS), tumours sympathetic system and Hirschsprung disease. In CCHS, prevalent mutation is an expansion 20-alanine stretch ranging from +5 to +13 alanines, whereas frameshift missense are found occasionally. To determine molecular basis impaired function, we assayed transactivation DNA binding properties...
Diabetic patients exhibit dysregulated inflammatory and immune responses that predispose them to chronic wound infections the threat of limb loss. The molecular underpinnings responsible for this have not been well elucidated, particularly in setting biofilms. This study evaluates host biofilm-impaired wounds using TallyHo mouse, a clinically relevant polygenic model type 2 diabetes. No differences cytokine or Toll-like receptor (TLR) expression were noted unwounded skin noninoculated...
Introduction The recent literature suggests that chronic wound biofilms often consist of multiple bacterial species. However, without appropriate in vivo, polybacterial biofilm models, our understanding these complex infections remains limited. We evaluate and compare the effect single- mixed-species on host healing dynamics using a quantitative, rabbit ear model. Methods Six-mm dermal punch wounds New Zealand ears were inoculated with Staphylococcus aureus strain UAMS-1, Pseudomonas...
Background: Bacterial biofilm is recognized as a major detriment to wound healing. The efficacy of traditional care against has never been studied. authors evaluated the effect clinical strategies biofilm-infected wounds in quantitative, vivo model. Methods: Using rabbit ear model, were inoculated with Pseudomonas aeruginosa or left uninfected controls. Inoculated acted untreated controls underwent treatment: every-other-day sharp débridement (I), lavage (II), Silvadene (III), and (IV),...
Blocking Na x expression improves scarring and atopic dermatitis–like symptoms by improving barrier function.
Abstract Breast implant rupture occurs in both saline and silicone implants, with estimated risk of between 5.3% 15.1% over a 10-year period. Concerns regarding the effect breast implants on immune system remain despite currently published data that does not support link ruptured or not, autoimmune symptoms. The authors aimed to determine if there were systemic local changes caused by rupture. Healthy females either intact recruited. Enzyme-linked immunosorbent assay (ELISA) was performed...
Background: 3-Hydroxy-3-methylglutaryl-coenzyme A reductase inhibitors, also known as statins, are the most commonly prescribed cholesterol-lowering medications in world. Statins have been shown to inhibit connective tissue growth factor (CTGF) gene and protein expression vitro, previous studies authors' laboratory demonstrated that CTGF plays a significant role wound healing scarring. The authors explore whether administration of various statins wounds has any effect on hypertrophic scar...
Background Approximately 15% of the United States population suffers from chronic kidney disease (CKD), often demonstrating an associated impairment in wound healing. This study outlines development a surgical murine model CKD order to investigate mechanisms underlying this impairment. Methods was induced mice by partial cauterization one cortex and contralateral nephrectomy, modifying previously published technique. After minimum 6-weeks, splinted, dorsal excisional wounds were created...
Abstract Bacterial biofilms, a critical chronic wound mediator, remain difficult to treat. Energy‐based devices may potentially improve healing, but with no evidence of efficacy against biofilms. This study evaluates noncontact, low‐frequency ultrasound ( NLFU ) in the treatment biofilm‐infected wounds. Six‐millimeter dermal punch wounds rabbit ears were inoculated 10 7 colony‐forming units P seudomonas aeruginosa or left as sterile controls. A biofilm was established vivo using our...
The importance of bacterial biofilms to chronic wound pathogenesis is well established. Different treatment modalities, including topical dressings, have yet show consistent efficacy against biofilm. This study evaluates the impact a novel, antimicrobial Test Dressing on Pseudomonas aeruginosa biofilm-infected wounds. Six-mm dermal punch wounds in rabbit ears were inoculated with 10(6) colony-forming units P. aeruginosa. Biofilm was established vivo using our published model. changes...
The retinoic acid‐inducible and developmentally regulated transcription factor AP‐2 plays an important role during development. In adult mammals, is expressed in both neural non‐neural tissues. However, the function of different neuronal phenotypes poorly understood. this study, transcriptional regulation tyrosine hydroxylase (TH) dopamine β‐hydroxylase (DBH) genes by was investigated. binding sites were identified upstream regions genes. Electrophoretic mobility shift assays (EMSA) DNase I...