A5038177729- Enzyme Production and Characterization
- Enzyme-mediated dye degradation
- Iron Metabolism and Disorders
- Advanced biosensing and bioanalysis techniques
- Enzyme Catalysis and Immobilization
- Microplastics and Plastic Pollution
- Graphene and Nanomaterials Applications
- Bacteriophages and microbial interactions
- Nanoparticle-Based Drug Delivery
- Trace Elements in Health
- Microbial Natural Products and Biosynthesis
- Chemical Synthesis and Analysis
- Biochemical and Structural Characterization
- Biochemical and biochemical processes
- Lignin and Wood Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Biofuel production and bioconversion
- Biosensors and Analytical Detection
- Microbial bioremediation and biosurfactants
- Genomics and Phylogenetic Studies
- Lanthanide and Transition Metal Complexes
- Antimicrobial Peptides and Activities
- Synthesis and Catalytic Reactions
- Marine Sponges and Natural Products
- Catalytic C–H Functionalization Methods
Agency for Science, Technology and Research
2015-2024
Singapore Institute of Technology
2024
Institute of Molecular and Cell Biology
2021-2023
Cell Biotech (South Korea)
2023
Biocat
2023
Nanyang Technological University
2010-2020
Nanyang Polytechnic
2014
Howard Hughes Medical Institute
2010
California Institute of Technology
2010
Jadavpur University
2005-2007
Owing to their small size, biocompatibility, unique and tunable photoluminescence, physicochemical properties, graphene quantum dots (GQDs) are an emerging class of zero‐dimensional materials promising a wide spectrum novel applications in bio‐imaging, optical, electrochemical sensors, energy devices, so forth. Their widespread use, however, is largely limited by the current lack high yield synthesis methods high‐quality GQDs. In this contribution, facile method electrochemically exfoliate...
The monolithic three-dimensional (3D) graphene network is used as the support for Pt nanoparticles (NPs) to fabricate an advanced 3D graphene-based electrocatalyst. Distinct from previous strategies, monodispersed NPs with ultrafine particle size (∼3 nm) are synthesized using ferritin protein nanocages template and subsequently self-assembled on by leveraging hydrophobic interaction between graphene. Following self-assembly, ferritins removed, resulting in a stable NP/3D composite. composite...
Bioengineered protein-based nanodevices with tunable and reproducible memristive performance are fabricated by combining the unique high loading capacity of Archaeoglobus fulgidus ferritin OWL-generated nanogaps. By tuning iron amount inside ferritin, ON/OFF ratio conductance switching can be modulated accordingly. Higher molecular exhibits better owing to higher electrochemical activity ferric complex core. As a service our authors readers, this journal provides supporting information...
Abstract Graphene quantum dots (GQDs) have been emerging as next‐generation bioimaging agents because of their intrinsic strong fluorescence, photostability, aqueous stability, biocompatibility, and facile synthesis. In this work, GQDs are encapsulated in ferritin protein nanocages to develop multi‐functional nanoplatforms toward multi‐modal imaging cancer therapy. Encapsulation ultra‐small is expected reduce quick excretion from the body increase efficiency. To expand functionality...
Self-assembling protein cages have been exploited as templates for nanoparticle synthesis. The ferritin molecule, a cage present in most living systems, stores excess soluble ferrous iron the form of an insoluble ferric complex within its cavity. Magnetic nanocores formed by loading engineered from Archaeoglobus fulgidus (AfFtn-AA) were studied potential magnetic resonance (MR) imaging contrast agent. self-assembly characteristics AfFtn-AA investigated using dynamic light scattering...
Archaeoglobus fulgidus ferritin (AfFtn) is the only tetracosameric known to form a tetrahedral cage, structure that remains unique in structural biology. As result of (2-3) symmetry, four openings (∼45 Å diameter) are formed cage. This open assembly contradicts paradigm typical cage: closed having octahedral (4-3-2) symmetry. To investigate molecular mechanism affecting this atypical assembly, amino acid residues Lys-150 and Arg-151 were replaced by alanine. The data presented here shed...
T(2) contrast is gaining importance in high field strength MRI. We report a strategy for developing agent from paramagnetic metal ions synthesized within an engineered protein cage. The manganese-ferritin nanocomposite showed relaxivity indicating its potential as ultrasensitive agent.
Lignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount lignin produced as by-product paper industry, while cellulosic components plant biomass are utilized pulp. In spite vast potential, remains least exploited component due to its extremely complex and heterogenous structure. Several enzymes have been reported lignin-degrading properties could be potentially used in biorefining if their catalytic improved by enzyme...
The electrons generated by photosynthetic water splitting have been studied for direct electron transfer under light irradiation.
The isolation of early intermediates in the laccase-catalysed oligomerisation lignin model compounds indicated preferential formation C<sub>5</sub>–C<sub>5′</sub> over C<sub>5</sub>–O–C<sub>4′</sub> linkages.
Polyethylene terephthalate (PET) hydrolase enzymes show promise for enzymatic PET degradation and green recycling of single-use vessels representing a major source global pollution. Their full potential can be unlocked with enzyme engineering to render activities on recalcitrant substrates commensurate cost-effective at scale. Thermostability is highly desirable property in industrial enzymes, often imparting increased robustness significantly reducing quantities required. To date, most...
In biomineralization processes, a supramolecular organic structure is often used as template for inorganic nanomaterial synthesis. The E2 protein cage derived from Geobacillus stearothermophilus pyruvate dehydrogenase and formed by the self‐assembly of 60 subunits, has been functionalized with non‐native iron‐mineralization capability incorporating two types iron‐binding peptides. peptides introduced at interior surface do not affect subunits. contrast to wild‐type, engineered cages can...
Abstract The laccase‐catalyzed oxidative polymerization of monomeric and dimeric lignin model compounds was carried out with oxygen as the oxidant in aqueous medium. oligomers were characterized by using gel permeation chromatography (GPC) matrix‐assisted laser desorption ionization time‐of‐flight mass spectroscopy (MALDI‐TOF MS) analysis. Oxidative led to formation oligomeric species a number‐average molecular weight ( M n ) that ranged from 700 2300 Da low polydispersity index....
Activating industrially important aromatic hydrocarbons by installing halogen atoms is extremely in organic synthesis and often improves the pharmacological properties of drug molecules. To this end, tryptophan halogenase enzymes are potentially valuable tools for regioselective halogenation arenes, including various indole derivatives similar scaffolds. Although endogenous show reasonable substrate scope towards compounds, their efficacy can be improved engineering. Using a structure-guided...