A5038044627- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
- RNA regulation and disease
- COVID-19 Clinical Research Studies
- Biosensors and Analytical Detection
Montana State University
2021-2023
Over 950,000 whole-genome sequences of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have been determined for viruses isolated from around the world. These are critical understanding spread and evolution SARS-CoV-2. Using global phylogenomics, we show that mutations frequently occur in C-terminal end ORF7a. We isolate one these mutant a patient sample use viral challenge experiments to link this (ORF7aΔ115) growth defect. ORF7a is implicated immune modulation, truncation...
Polymerase Chain Reaction (PCR) is the reigning gold standard for molecular diagnostics. However, SARS-CoV-2 pandemic reveals an urgent need new diagnostics that provide users with immediate results without complex procedures or sophisticated equipment. These demands have stimulated a tsunami of innovations improve turnaround times compromising specificity and sensitivity has established PCR as paragon Here we briefly introduce origins isothermal amplification, before turning to emergence...
Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we repurpose the type III-A CRISPR complex from Thermus thermophilus (TtCsm) programmable capture and concentration specific RNAs mixtures. The target bound TtCsm generates two cyclic oligoadenylates (i.e., cA
CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for RNA remain limited. Here, we combine sequence-specific cleavage by ribonucleases with programmable repair to make deletions and insertions in RNA. This work establishes a recombinant technology immediate applications the facile engineering viruses.
Type-III CRISPR-Cas systems have recently been adopted for sequence-specific detection of SARS-CoV-2. Here, we make two major advances that simultaneously limit sample handling and significantly enhance the sensitivity SARS-CoV-2 RNA directly from patient samples. First, repurpose type III-A CRISPR complex Thermus thermophilus (TtCsm) programmable capture concentration specific RNAs mixtures. The target bound TtCsm primarily generates cyclic oligoadenylates (i.e., cA3 cA4) allosterically...
Abstract Over 200,000 whole genome sequences of SARS-CoV-2 have been determined for viruses isolated from around the world. These critical understanding spread and evolution SARS-CoV-2. Using global phylogenomics, we show that mutations frequently occur in C-terminal end ORF7a. We one these mutant a patient sample used viral chal-lenge experiments to demonstrate Δ115 mutation results growth defect. ORF7a has implicated immune modulation, truncation distinct changes interferon stimulated gene...
Abstract CRISPR RNA-guided endonucleases have enabled precise editing of DNA. However, options for RNA remain limited. Here, we combine sequence-specific cleavage by ribonucleases with programmable repair to make deletions and insertions in RNA. This work establishes a new recombinant technology immediate applications the facile engineering viruses. One-Sentence Summary Programmable enable technology.
Over 200,000 whole genome sequences of SARS-CoV-2 have been determined for viruses isolated from around the world. These critical understanding spread and evolution SARS-CoV-2. Using global phylogenomics, we show that mutations frequently occur in C-terminal end ORF7a. We isolate one these mutant a patient sample use viral challenge experiments to demonstrate this mutation results growth defect. ORF7a has implicated immune modulation, truncation distinct changes interferon stimulated gene...