Keratinocytes and dermal endothelial cells, excluding leukocytes that infiltrate wounds, are the main source of soluble factors regulating healing skin ulcers. We used immunohistochemistry to analyze expression various chemotactic growth their receptors in margin diabetic foot ulcers normal nondiabetic skin. Our study found significantly elevated transforming factor-beta1 (TGF-beta1) type I TGF-beta (TGFbetaR1), granulocyte macrophage colony-stimulating factor (GM-CSF), epidermal (EGF)...

Although there is some unevenness in the chapters, most are excellent, and I strongly recommend that all diagnostic laboratories should have access to a copy.

The extent to which plasma HDL concentration regulates reverse cholesterol transport (RCT) is not known. principal acceptors of unesterified (UC) from cultured cells are small pre-β-HDL, we have shown increase in during intravenous infusion apolipoprotein A-I/phosphatidylcholine (apoA-I/PC) discs humans. We now examined the effects on tissue fluid and RCT. ApoA-I/PC or proapoA-I/PC were infused into 16 healthy males. Eleven had been given radiocholesterol label pools; 12 prenodal leg lymph...

Changes in normal human leg lymph protein concentration, output, and flow/lymph concentration relationship, as well lactate dehydrogenase alkaline phosphatase activity were followed during procedures known to increase capillary filtration venous stasis, muscular exercise warming of tissues. Lymph flow increased by 83% two hour ergometer cycling, 117% warm water foot bath. During a period stasis dropped 50%. There was an the rest following all three types experiment, most pronounced after...

Journal Article Surgical lymphaticovenous shunts in patients with secondary lymphoedema Get access J Nielubowicz, Nielubowicz Research Laboratory, Polish Academy of Sciences, Warsaw, Poland Search for other works by this author on: Oxford Academic Google Scholar W Olszewski British Surgery, Volume 55, Issue 6, June 1968, Pages 440–442, https://doi.org/10.1002/bjs.1800550609 Published: 06 December 2005

Background: The manual lymphatic drainage in lymphedema has proved to be successful; however, this method cannot applied millions of patients around the world. only solution is offer inexpensive, easily accessible mechanical devices for pneumatic compression (IPC). These should designed on parameters edema fluid hydromechanics. Recent data point high pressures and long time compression. Aim: To validate effects 3 years daily pressure, inflation IPC therapy terms decrease limb...

Peptides secreted by adipose tissue (adipokines) may enter blood via capillaries or lymph. The relative importance of these pathways for a given adipokine might influence its biological effects. Because this has not been studied in any species, we measured the concentrations seven adipokines and eight nonsecreted proteins afferent peripheral lymph venous plasma from 12 healthy men. Data were used to derive indices microvascular permeability, which conjunction with molecular radii estimate...

Lymphedema (LE) is a morbid disease characterized by chronic limb swelling and adipose deposition. Although it clear that lymphatic injury necessary for this pathology, the mechanisms underlie lymphedema remain unknown. IL-6 known regulator of homeostasis in obesity has been shown to be increased primary secondary models lymphedema. Therefore, purpose study was determine role deposition The expression analyzed clinical tissue specimens serum from patients with or without LE, as well two...

Filarial lymphedema is complicated by frequent episodes of dermatolymphangioadenitis (DLA). It not certain whether DLA filarial or bacterial etiology. The frequency episodic does depend on the presence absence microfilariae. Antibiotic therapy effective in prevention and treatment DLA. These observations point to rather than etiology Skin lymph node biopsies, tissue fluid, lymph, blood from patients with chronic lymphedema, during acute DLA, were cultured for detection bacteria. A high...

Human leg lymphatics contract spontaneously, rhythmically propelling lymph. This intrinsic property regulates the fluid environment in intercellular space skin, connective tissue, and perivascular spaces. The pressures generated by lymphatic contractions constitute main force for lymph flow. mechanism is of utmost importance during night rest, anesthesia, immobilization, as well those with damaged peripheral motor neurons. All supporting forces are only secondary to created spontaneous...

Abstract The twenty‐four hour variation in concentration and output of total protein, lactate dehydrogenase alkaline phosphatase has been studied the peripheral lymph from legs 5 healthy volunteers over a period five days. highest these proteins was found collected during first two hours after night's rest. During day continuous decrease occurred without any direct correlation to flow. protein between early morning late about 40 %, 90% 45 o , respectively.

Rheumatoid arthritis (RA) is characterized by inflammatory reactions in joints and adjacent tissues unaccompanied clinically evident changes lymphatics lymph nodes draining the inflamed areas. The explanation for this phenomenon, which contrasts with infectious processes soft that evoke major lymphatic system, unclear. To determine factors produced of RA patients are transported to nodes, we measured levels immunoglobulins, cytokines, chemokines prenodal from foot quantified their rate...

Normal human peritoneal cells collected during elective laparatomy from patients with gallbladder stones without clinically detectable inflammatory changes were characterized phenotypically immunocytochemical method and flow cytometry, special attention paid to the presence of memory cells. The responsiveness normal PCs mitogen and, specifically, role macrophages in this process was studied. consisted 45% monocytes/ (CD68+), as many CD2+ T lymphocytes, 8% CD57+ NK K 2% CD22+ B, CD4/CD8 ratio...

Background The pre-nodal afferent lymph is the fluid which directly derives from extracellular milieu every parenchymal organ and, as it continues to circulate between cells, collects products deriving metabolism/catabolism. A comprehensive qualitative and quantitative investigation of self-antigenic repertoire transported by human still missing. Methodology/Principal Findings major difference plasma could be visualized FPLC 2D gel in amount low molecular weight corresponding peptide...

Pneumatic compression of tissues with lymph stasis is, aside from the manual massage, a commonly used therapeutic modality in limb lymphedema. A number pneumatic devices have been constructed. There is lack reports comparative studies determining inflation pressure levels, inflation/deflation cycle times, and total pumping times. Aim: We tried to answer question how high long timing should be applied soft reach tissue fluid (TF) head above 30 mmHg, necessary initiate proximal flow. Methods:...

This study indicates that circulating progenitors of bone marrow origin give rise to cells with smooth muscle-like properties during formation neointimal thickenings in the arterial wall after allotransplantation and balloon injury. A segment abdominal aorta was transplanted from female F344 male LEW rats, grafts were analyzed for by using SRY gene as a marker. Immunostaining demonstrated CD45-positive leukocytes made up 35–45% 8-week period examined. Concurrently, 70% host origin, shown...

Peripheral lymph lipoproteins have been characterized in animals, but there is little information about their composition, and none ultrastructure, normal humans. Therefore, we collected afferent leg from 16 healthy males quantified lipids apolipoproteins fractions separated by high performance-size exclusion chromatography. Apolipoprotein B (apoB) was found almost exclusively low density lipoproteins. The distribution of apoA-I, particularly lipoprotein A-I (LpA-I) without A-II particles,...