A5100680253- Food Allergy and Anaphylaxis Research
- Alzheimer's disease research and treatments
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Occupational exposure and asthma
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- Cholinesterase and Neurodegenerative Diseases
- Contact Dermatitis and Allergies
- Moyamoya disease diagnosis and treatment
- Cerebrovascular and genetic disorders
- Saffron Plant Research Studies
- Genetically Modified Organisms Research
- Analytical Chemistry and Sensors
- Marine Sponges and Natural Products
- Computational Drug Discovery Methods
- Cardiac Fibrosis and Remodeling
- Protein Degradation and Inhibitors
- Biological Control of Invasive Species
- Folate and B Vitamins Research
- Phytase and its Applications
- Electrochemical sensors and biosensors
- stochastic dynamics and bifurcation
- Metabolism and Genetic Disorders
Nanjing Medical University
2025
Jiangsu Province Hospital
2025
Jinan University
2023-2024
National Clinical Research Center for Digestive Diseases
2024
Beijing Tian Tan Hospital
2024
Capital Medical University
2014-2024
Sun Yat-sen University
2019-2023
Shanxi Medical University
2023
Beihua University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Abstract Whether stem-cell-like cancer cells avert ferroptosis to mediate therapy resistance remains unclear. In this study, using a soft fibrin gel culture system, we found that tumor-repopulating (TRCs) with cell characteristics resist chemotherapy and radiotherapy by decreasing sensitivity. Mechanistically, through quantitative mass spectrometry lipidomic analysis, determined mitochondria metabolic kinase PCK2 phosphorylates activates ACSL4 drive ferroptosis-associated phospholipid...
Deposition of aggregated amyloid β-protein (Aβ), a proteolytic cleavage product the precursor protein (1), is critical step in development Alzheimer's disease (2). However, we are far from understanding molecular mechanisms underlying initiation Aβ polymerization <i>in vivo</i>. Here, report that seeding Aβ, which catalyzes fibrillogenesis soluble generated apically missorted cultured epithelial cells. Furthermore, generation this depends exclusively on presence cholesterol Taken together...
Abstract Cut gardenia ( Gardenia jasminoides Ellis) foliage is widely used as a vase material or flower bouquet indoors; however, insufficient indoor light accelerates its senescence, which shortens viewing time. In this study, applying melatonin to delay leaf senescence when exposed extremely low condition (darkness), and the results showed that 1.0 mM was effective concentration. At concentration, chlorophyll contents fluorescence parameters (F v /F m , F 0 Y(II)) increased, while...
Biliary tract cancer (BTC) has greatly influenced patient survival for years. Nowadays, immunotherapy represents a promising breakthrough and proteomics is one of powerful technologies in biomarker research. We collected plasma tissue samples from 37 patients with advanced BTC 92 proteins were analyzed by proximity extension assay (PEA). Through linear mixed effect models, compared to partial response (PR) group, 8 proteins, IL7, ANGPT2, IL15, HO-1, CXCL1, CXCL5, IL33, VEGFA, exhibited...
A planar amperometric glucose microsensor based on oxidase immobilized by chitosan film Prussian Blue layer has been developed. The experimental results show that the optimum detection potential is 50 mV (versus Ag/AgCl) and pH 6.5. Under selective conditions sensor exhibits excellent sensitivity of 98 nA/M a linear range 0.1-6.0 mM. apparent Michanelis-Menten constant 21 response time less than 60 seconds. No change in to was observed during one month. Foremost, interference ascorbic uric...
Susceptibility to pepsin digestion of candidate transgene products is regarded an important parameter in the weight-of-evidence approach for allergenicity risk assessment genetically modified crops. It has been argued that protocols used this should better reflect physiological conditions encountered representative food consumption scenarios. To evaluate whether inclusion more conditions, such as sub-optimal and lower concentrations, combination with pancreatin digestion, improved...
Familial Alzheimer disease-causing mutations in the presenilins increase production of longer pathogenic amyloid β-peptides (Aβ42/43) by altering γ-secretase activity. The mechanism underlying this effect remains unknown, although it has been proposed that heteromeric macromolecular complexes containing mediate cleavage β-precursor protein. Using a random mutagenesis screen presenilin-1 (PS1) for PS1 endoproteolysis-impairing mutations, we identified five unique mutants, including R278I-PS1...
The susceptibility of a dietary protein to proteolytic degradation by digestive enzymes, such as gastric pepsin, provides information on the likelihood systemic exposure structurally intact and biologically active macromolecule, thus informing safety proteins for human animal consumption. Therefore, purpose standardized in vitro studies that are performed during assessments is distinguish whether interest susceptible or resistant pepsin via study design enables study-to-study comparison....
Galanthamine, one kind of Amaryllidaceae alkaloid extracted from the Lycoris species, is used in treatment Alzheimer's disease. In regards to medical and economic importance, biosynthesis regulatory mechanism secondary metabolites remain uninvestigated.BLAST was identify sequence tyrosine decarboxylase transcriptome aurea (L'Hér) Herb. The enzyme activity this TYDC determined by using heterologous expressed protein Escherichia coli cells. related productive contents tyramine were detected...
The susceptibility of newly expressed proteins to digestion by gastrointestinal proteases (e.g., pepsin) has long been regarded as one the important endpoints in weight-of-evidence (WOE) approach assess allergenic risk genetically modified (GM) crops. European Food Safety Authority (EFSA) suggested that current study protocols used for this assessment should be more accurately reflect diverse physiological conditions encountered human populations and post-digestion analysis include...
The phytochemical investigation of the roots Croton crassifolius led to isolation 16 new halimane furanoditerpenoids, crohalifuranes A–P (1–16), along with 15 known analogues, 17–31. structures including their absolute configurations were elucidated by NMR and MS data analysis, comparison experimental calculated electronic circular dichroism data, single-crystal X-ray diffraction chemical methods. Crohalifuranes A (1) B (2) are tetranor- 19-nor-halimane diterpenoids featuring a rare...
Integrin αV gene expression is often dysregulated in cancers especially hepatocellular carcinoma (HCC); however, the mechanism of regulation poorly understood. Here, it demonstrated that sulfatide activated integrin transcription, through histone H3K9/14 acetylation at promoter, and high are closely associated with poor prognosis. To elucidate acetylation, sulfatide-bound proteins were screened by mass spectrometry (MS), bromodomain containing protein 1 (BRD1) was identified as an...
Abstract Moyamoya disease (MMD) is a cerebrovascular narrowing and occlusive condition characterized by progressive stenosis of the terminal portion internal carotid artery formation an abnormal network dilated, fragile perforators at base brain. However, role PANoptosis, apoptotic mechanism associated with vascular disease, has not been elucidated in MMD. In our study, total 40 patients’ genetic data were included, 815 MMD-related differential genes screened, including 215 upregulated 600...
Mitochondrial DNA (mtDNA) copy number (CN) is a biomarker of mitochondrial function and has been reported associated with kidney disease. However, its association IgA nephropathy (IgAN), the most common cause glomerulonephritis (GN), not evaluated. We included 664 patients biopsy-proven IgAN measured mtDNA-CN in peripheral blood by multiplexed real-time quantitative polymerase chain reaction (RT-qPCR). examined associations between clinical variables found that higher had estimated...
The integration of omic data with metabolic networks has been demonstrated to be an effective approach elucidate the underlying mechanisms in life. Because pathways Thermoanaerobacter tengcongensis (T. tengcongensis) are incomplete, we used a 1-(13)C-glucose culture monitor intracellular isotope-labeled metabolites by GC/MS and identified gap gene glucose catabolism, Re-citrate synthase. Based on genome annotation biochemical information, reconstructed network metabolism amino acid synthesis...
Background:Alzheimer-associated neuronal thread protein (AD7c-NTP) has been found to be a candidate biomarker of Alzheimer's disease (AD). Objective:To investigate the effects urine collected time, different preservatives addition, and storage condition on measurement AD7c-NTP by en zyme-linked immunosorbent assay (ELISA). Methods:Three hundred samples were from 20 participants at three time points five consecutive days. These immediately placed 4°C detected within 2 h. The single spot first...
The γ-secretase complex, composed of presenilin, nicastrin (NCT), anterior pharynx-defective 1 (APH-1), and presenilin enhancer 2 (PEN-2), is assembled in a highly regulated manner catalyzes the intramembranous proteolysis many type I membrane proteins, including Notch amyloid precursor protein. family receptors plays important roles cell fate specification during development adult tissues, aberrant hyperactive signaling causes some forms cancer. γ-Secretase-mediated processing at surface...
A germacrane sesquiterpenoid library containing 30 compounds (2-31) was constructed by structural modification of a major component aristolactone (1) from the traditional Chinese medicine Aristolochia yunnanensis. Compound 11 identified as promising anticardiac fibrosis agent systematic screening this library. could inhibit expression fibronectin (FN), α-smooth muscle actin (α-SMA), and collagens in transforming growth factor β 1 (TGFβ1)-stimulated cardiac fibroblasts at micromolar level...
Western corn rootworm (WCR), Diabrotica virgifera virgifera, LeConte, is an insect pest that poses a significant threat to the productivity of modern agriculture, causing economic and crop losses. The development genetically modified (GM) crops expressing one or more proteins confer tolerance specific pests, such as WCR, was historic breakthrough in agricultural biotechnology continues serve invaluable tool management. Despite this, evolving resistance existing control expressed current...
Introduction: Moyamoya disease (MMD) is a chronic cerebrovascular that can lead to ischemia and hemorrhagic stroke. The relationship between oxidative phosphorylation (OXPHOS) MMD pathogenesis remains unknown. Methods: gene expression data of 60 participants were acquired from three Gene Expression Omnibus (GEO) datasets, including 36 24 in the control groups. Differentially expressed genes (DEGs) patients groups identified. Machine learning was used select key OXPHOS-related associated with...
Background We showed in our previous study that the N-terminal 17-mer peptide of amyloid precursor protein (APP17-mer peptide), an active segment with trophic and antioxidative effects, protects skin fibroblasts against ultraviolet (UV) damage downregulates matrix metalloproteinase 1 (MMP-1) expression. The aim current was to explore protective effects P165, 5-mer analog is resistant enzymolysis, on UVA-induced human dermal (HDFs). Methods HDFs were cultured Dulbecco’s modified Eagle’s...