A5103259596- Endoplasmic Reticulum Stress and Disease
- Heat shock proteins research
- Crystallization and Solubility Studies
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Chemical and Physical Properties in Aqueous Solutions
- Thermodynamic properties of mixtures
- X-ray Diffraction in Crystallography
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Alzheimer's disease research and treatments
- Cancer, Hypoxia, and Metabolism
- Chemical Thermodynamics and Molecular Structure
- Cell death mechanisms and regulation
- Ovarian function and disorders
- Catalytic Alkyne Reactions
- Chemical synthesis and alkaloids
- FOXO transcription factor regulation
- Synthesis and bioactivity of alkaloids
- Catalysis for Biomass Conversion
- RNA and protein synthesis mechanisms
- Radiation Therapy and Dosimetry
- Reproductive Biology and Fertility
- Glutathione Transferases and Polymorphisms
- Catalysis and Hydrodesulfurization Studies
China Medical University
2007-2024
Second Xiangya Hospital of Central South University
2020-2023
Central South University
2020-2023
Huanghuai University
2020
Wenzhou University
2014-2015
Nanjing University
2003-2014
First Hospital of China Medical University
2007-2014
Beijing University of Chemical Technology
2014
Ministry of Education of the People's Republic of China
2013
Center for Excellence in Molecular Cell Science
2013
Aerobic glycolysis, a phenomenon known historically as the Warburg effect, is one of hallmarks cancer cells. In this study, we characterized role BAG3 in aerobic glycolysis pancreatic ductal adenocarcinoma (PDAC) and its molecular mechanisms. Our data show that aberrant expression significantly contributes to reprogramming glucose metabolism PDAC Mechanistically, increased Hexokinase 2 (HK2) expression, first key enzyme involved at posttranscriptional level. interacted with HK2 mRNA, degree...
Emerging lines of evidence have shown that blockade ubiquitin-proteasome system (UPS) activates autophagy. The molecular players regulate the relationship between them remain to be elucidated. Bcl-2 associated athanogene 3 (BAG3) is a member BAG co-chaperone family regulates ATPase activity heat shock protein 70 (HSP70) chaperone family. Studies on BAG3 demonstrated it plays multiple roles in physiological and pathological processes, including antiapoptotic activity, signal transduction,...
Proteasome inhibitors represent a novel class of antitumor agents with preclinical and clinical evidence activity against hematological malignancies solid tumors. Emerging lines suggest that the unfolded protein response is implicated in proteasome inhibitors-induced apoptosis. Glucose-regulated 78 kDa (GRP78) CCAAT/enhancer-binding homologous (CHOP) as part play critical roles cell survival or death. Here we demonstrate induction GRP78 CHOP are differently regulated upon inhibition...
Bcl-2 associated athanogene 3 (BAG3) is highly expressed in pancreatic ductal adenocarcinoma (PDAC), and its high expression appears to be a poor prognostic factor for patients with PDAC. In this study, we show that BAG3 knockdown significantly decreases migration invasion of PDACs via reduction interleukine-8 (IL-8) production. regulates IL-8 at the posttranscriptional levels interplay between recruitment RNA-binding protein HuR miR-4312. binds cis-elements located 3'-untranslational region...
Familial Alzheimer disease-causing mutations in the presenilins increase production of longer pathogenic amyloid β-peptides (Aβ42/43) by altering γ-secretase activity. The mechanism underlying this effect remains unknown, although it has been proposed that heteromeric macromolecular complexes containing mediate cleavage β-precursor protein. Using a random mutagenesis screen presenilin-1 (PS1) for PS1 endoproteolysis-impairing mutations, we identified five unique mutants, including R278I-PS1...
// De-Hui Kong 1, 2 , Si Li 1 Zhen-Xian Du 3 Chuan Liu 4 Bao-Qin Chao Zhi-Hong Zong Hua-Qin Wang Department of Biochemistry and Molecular Biology, China Medical University, Shenyang, Key Laboratory Cell Ministry Public Health, Education, Endocrinology Metabolism, The 1st Affiliated Hospital, Gynecology Obstetrics, Shengjing Correspondence to: Wang, e-mail: wanghq_doctor@hotmail.com Keywords: BAG3, HCCs, G6PD, pentose phosphate pathway Received: April 26, 2015 Accepted: November 14,...
Context: BAG3 plays a regulatory role in number of cellular processes. Recent studies have attracted much attention on its activation selective autophagy. In addition, we very recently reported that is implicated BECN1-independent autophagy, namely noncanonical Objective: The current study aimed to investigate the potential involvement canonical autophagy triggered by Earle's Balanced Salt Solution (EBSS) starvation. Setting and Design: Replacement complete medium with EBSS was used trigger...
Abstract BAG3 is an evolutionarily conserved co-chaperone expressed at high levels and has a prosurvival role in many tumor types. The current study reported that was induced under specific floating culture conditions enrich breast cancer stem cell (BCSC)-like cells spheres. Ectopic overexpression increased CD44 + /CD24 − CSC subpopulations, first-generation second-generation mammosphere formation, indicating promotes self-renewal maintenance cancer. We further demonstrated mechanically,...
Abstract Background The ubiquitin-proteasome system and macroautophagy (hereafter referred to autophagy) are two complementary pathways for protein degradation. Emerging evidence suggests that proteasome inhibition might be a promising approach tumor therapy. Accumulating data suggest autophagy is activated as compensatory mechanism upon activity impaired. Method Autophagy activation was measured using acridine orange staining LC3 transition. Cell viability apoptosis were MTT assay flow...
Proteasome inhibitors represent a novel class of antitumor agents with pre-clinical and clinical evidence activity against hematologic malignancies solid tumors. However, emerging indicates that antiapoptotic factors may also accumulate as consequence exposure to these drugs, thus it seems plausible the activation survival signaling cascades might compromise their antitumoral effects. Peroxiredoxins (PRDXs) are family thiol-containing peroxidases identified primarily by ability remove...
Abstract The presenilin (PS) complex, including PS, nicastrin, APH‐1 and PEN‐2, is essential for γ‐secretase activity, which required amyloid β‐protein (Aβ) generation. However, the precise individual roles of three cofactors in PS complex Aβ generation remain to be clarified. Here, distinguish activity from those endoproteolysis, we investigated their reconstituted by coexpression N‐ C‐terminal fragments (NTF CTF) PS‐null cells. We demonstrate that PS1 NTF CTF forms heterodimer restores was...
Context: The inhibition of the 26S proteasome may lead to endoplasmic reticulum stress, which has been shown be implicated in antitumoral effects inhibitors. Oxygen-regulated protein 150 (ORP150) is an inducible chaperone that up-regulated after numerous cellular insults and a cytoprotective role for maintenance viability.
An expedient approach was developed to construct the 11H-indolo[3,2-c]quinoline scaffold starting from acyclic alkyne substrates. The five- and six-membered nitrogen-containing rings in tetracyclic skeleton were elaborated efficiently by gold(III)-catalyzed 5-endo-dig cyclization Hendrickson reagent promoted regioselective 6-endo cyclization.
Solid tumour frequently undergoes metabolic stress during development because of inadequate blood supply and the high nutrient expenditure. p53 is activated by glucose limitation maintains cell survival via triggering checkpoint. However, exact downstream contributors are not completely identified. BAG3 a cochaperone with multiple cellular functions implicated in reprogramming pancreatic cancer cells. The current study demonstrated that transcriptionally suppressed expression p53-dependent...
Pancreatic stellate cells (PSCs) are a subset of pancreatic cancer-associated fibroblasts, which play critical role in fibrosis, characteristic feature cancer. The interplay between PSCs and cancer is vital for promotion tumor progression metastasis. BAG3 correlated with poor prognostics patients ductal adenocarcinoma (PDAC), however, the exact mechanisms remain largely unknown. In this study, we demonstrated that downregulation decreased IL6 release by PDACs, reduction was, at least...
The presenilin (PS) complex, including PS, nicastrin (NCT), APH‐1 and PEN‐2, is essential for γ‐secretase activity. Previously, the PS C‐terminal tail was shown to be Here, further understand precise mechanism underlying activation of regulated by cofactors, we focused on role PS1 region transmembrane domain (TM) 8 in For this purpose, co‐expressed C‐terminally truncated (PS1ΔC) completely lacking activity short fragment PS‐null cells, because successful reconstitution cells co‐expression...