A5069713423- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Mesenchymal stem cell research
- Proteoglycans and glycosaminoglycans research
- MicroRNA in disease regulation
- Autophagy in Disease and Therapy
- Osteoarthritis Treatment and Mechanisms
- Corneal Surgery and Treatments
- Genomics, phytochemicals, and oxidative stress
- RNA modifications and cancer
- Curcumin's Biomedical Applications
- Telomeres, Telomerase, and Senescence
- Protease and Inhibitor Mechanisms
- Pharmacological Effects of Medicinal Plants
- Immune cells in cancer
- Neurogenesis and neuroplasticity mechanisms
- Bone Metabolism and Diseases
- Calpain Protease Function and Regulation
- Electrostatic Discharge in Electronics
- Fungal Biology and Applications
- Chemokine receptors and signaling
- Diabetes and associated disorders
- Cholesterol and Lipid Metabolism
- Ubiquitin and proteasome pathways
- Carbon and Quantum Dots Applications
Zunyi Medical University
2011-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2024
Institute of Hematology & Blood Diseases Hospital
2024
Sichuan University
2015-2024
Affiliated Hospital of Zunyi Medical College
2022
State Key Laboratory of Biotherapy
2015
Nanjing General Hospital of Nanjing Military Command
2014
Osaka University
2014
Nanjing University
2014
University of Notre Dame
2014
Our study first proposed that curcumin could protect human endothelial cells from the damage caused by oxidative stress via autophagy. Furthermore, our results revealed causes some novel cellular mechanisms promote autophagy as a protective effect. Pretreatment with remarkably improves survival of umbilical vein (HUVECs) H 2O 2-induced viability loss, which specifically evokes an autophagic response. Exposed to 2, curcumin-treated HUVECs upregulate level microtubule-associated protein 1...
Abstract Stem cell senescence is an important cause of aging. Delaying may present a novel way to combat aging and age‐associated diseases. This study provided mechanistic insight into the protective effect ganoderic acid D (GA‐D) against human amniotic mesenchymal stem (hAMSCs) senescence. GA‐D, Ganoderma lucidum ‐derived triterpenoid, markedly prevented hAMSCs via activating Ca 2+ calmodulin (CaM)/CaM‐dependent protein kinase II (CaMKII)/nuclear erythroid 2‐related factor 2 (Nrf2) axis,...
Despite unsurpassed anti-tumor activity of bortezomib for multiple myeloma (MM), drug resistance has emerged as a challenge, especially when MM cells adhere to the stroma. This study aimed determine whether bone marrow stromal (BMSCs) have role in development chemoresistance MM. Our data demonstrate that secretion interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), and cell-to-cell contact with microenvironment-derived from patients (MM-BMSCs) significantly decreased sensitivity...
Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by high levels of proinflammatory cytokines and epithelial barrier dysfunction. The root Ligularia fischeri (Ledeb.) Turcz. traditional Chinese medicinal herb with diverse therapeutic properties, which has been successfully used to treat inflammation-related diseases. However, little known about its effect mechanism against UC.To investigate the efficacy L. extracts UC.L. samples were prepared using alcohol...
<title>Abstract</title> <bold>Background</bold>: In the in-vitro expansion of mesenchymal stem cells (MSCs), replicative or stress-induced senescence poses a significant challenge, leading to loss their cellular properties and therapeutic functions. Currently, there is lack efficient strategies address this issue. <bold>Methods</bold>: Here we presented novel approach combat promote cell proliferation by coculturing human MSCs with peripheral blood lymphocytes (PBLs). After treatment PBL...
Aging is an important risk factor in the occurrence of many chronic diseases. Senescence and exhaustion adult stem cells are considered as a hallmark aging organisms. In this study, senescent human amniotic mesenchymal cell (hAMSC) model subjected to oxidative stress was established vitro using hydrogen peroxide. We investigated effects ganoderic acid D (GA-D), natural triterpenoid compound produced from Ganoderma lucidum , on hAMSC senescence. GA-D significantly inhibited β -galactosidase...
The cystine-cystine (CC) chemokine monocyte chemoattractant protein-1 (MCP-1) has been established playing a pathogenic role in the development of atherosclerosis due to its chemotactic ability leading monocytes locate subendothelia. Recent studies have revealed more MCP-1 functions other than chemotaxis. Here we reported that various concentrations (0.1-100 ng/ml) induced human umbilical vein endothelial cell (HUVEC) strain CRL-1730 apoptosis, caspase-9 activation, and couple mitochondrial...
Osteoarthritis (OA) affects a large number of patients; however, human umbilical cord stem cells exhibit therapeutic potential for treating OA. The aim the present study was to explore interaction between and degenerated chondrocytes, on chondrocytes. Human cord‑derived mesenchymal (hUC‑MSCs) were harvested from cords, flow cytometry used analyze surface antigen markers, in addition, chondrogenic, osteogenic adipogenic differentiation investigated. OA at P3 cocultured with hUC‑MSCs separated...
Abstract ZNF32 is a recently identified zinc finger protein and its functions remain largely unknown. Autophagy has been shown to affect cell proliferation survival. Here, we innovatively show the effect of on autophagy autophagy-associated death in breast carcinoma cells also elucidate underlying mechanisms. We examined autophagic activity LC3 II expression human lines with increased or decreased expression. Pharmacological inhibition (rapamycin) activation (EGF) assays were used...
CD44 antigen (CD44) is a transmembrane protein found in cell adhesion molecules and involved the regulation of various physiological processes cells. It was hypothesized that directly affected chondrogenic differentiation human amniotic mesenchymal stem cells (hAMSCs). In present study, expression chondrocyte‑associated factors detected absence presence antibody blocker anti‑CD44 during hAMSCs. Following inhibition expression, transcriptional levels genes SRY‑box transcription factor 9,...
Abstract Background The ubiquitin-proteasome system and macroautophagy (hereafter referred to autophagy) are two complementary pathways for protein degradation. Emerging evidence suggests that proteasome inhibition might be a promising approach tumor therapy. Accumulating data suggest autophagy is activated as compensatory mechanism upon activity impaired. Method Autophagy activation was measured using acridine orange staining LC3 transition. Cell viability apoptosis were MTT assay flow...
Human amniotic epithelial cells (hAECs) are a useful and noncontroversial source of stem for cell therapy regenerative medicine, but their limited proliferative ability hinders the acquisition adequate quantities clinical use due to not expressing telomerase in hAECs. Our previous study showed that hyaluronic acid (HA), an important component extracellular matrix, promoted proliferation human mesenchymal cells. Herein, we hypothesize HA might improve capability In present study, role on...
JS-K, a nitric oxide-releasing diazeniumdiolates, is effective against various tumors. We have discovered that JS-K was Hepatitis B virus (HBV)-positive HepG2.2.15 cells. This study used iTRAQ to identify differentially expressed proteins following treatment of Silenced Transgelin (shTAGLN-2.15) cells were constructed, and the cell viability analyzed by CCK8 assay after with JS-K. There 182 in treated-HepG2.2.15 cells; 73 up-regulated 109 down-regulated. These categorized according GO...
Osteoporosis is a common disease characterized by skeletal fragility and microarchitectural deterioration. However, existing conventional drugs exhibit limited efficacy can elicit severe adverse effects; moreover, novel stem cell-based therapies have not exhibited sufficient therapeutic efficacy. Our hypothesis that an appropriate osteogenic inducer may improve their In this study, we found bisdemethoxycurcumin (BDMC) stimulates the differentiation of human amniotic mesenchymal cells...
Realgar (As 4 S ) has been used in traditional Chinese medicines for treatment of malignancies. The poor solubility As hampered its clinical applications. quantum dots (RQDs) were developed to overcome these problems. Previous studies revealed that the RQDs effective against endometrial cancer JEC cells and hepatocarcinoma HepG2 via inducing apoptosis.Apoptosis autophagy are important programmed cell death pathways leading anticancer effects. This study further examined effects on autophagy,...
Although stem cell transplantation is an effective strategy in the treatment of type 1 diabetes mellitus (T1DM), mechanisms underlying its therapeutic effects remain unclear. We hypothesized that cells target gut microbiota and intestinal mucosal immunity to promote against T1DM. investigated human amniotic mesenchymal (hAMSCs) on streptozotocin-induced T1DM mice. hAMSCs promoted significant reductions blood glucose levels increased number insulin-secreting model. Compared with model mice,...
The replicative senescence of human amniotic epithelial stem cells (hAECs) is a major concern towards its clinical application. This study found that 300-kDa hyaluronic acid (HA) could effectively delay the hAECs, as indicated by downregulation cellular markers and alteration cell cycle, substantially improve differentiation capacities hAECs. HA was confirmed to regulate CD44 isoform switch upregulating CD44s downregulating CD44v, thus exerting an anti-aging effect. We further induced...