Abstract Stem cell senescence is an important cause of aging. Delaying may present a novel way to combat aging and age‐associated diseases. This study provided mechanistic insight into the protective effect ganoderic acid D (GA‐D) against human amniotic mesenchymal stem (hAMSCs) senescence. GA‐D, Ganoderma lucidum ‐derived triterpenoid, markedly prevented hAMSCs via activating Ca 2+ calmodulin (CaM)/CaM‐dependent protein kinase II (CaMKII)/nuclear erythroid 2‐related factor 2 (Nrf2) axis,...

Abstract MicroRNAs (miRNA) have been shown to be associated with tumor diagnosis, prognosis, and therapeutic response. MiR-328-3p plays a significant role in breast cancer growth; however, its actual function how it modulates specific biological functions is poorly understood. Here, miR-328-3p was significantly downregulated cancer, especially patients metastasis. Mitochondrial carnitine palmitoyl transferase 1a (CPT1A) downstream target gene the miR-328-3p-regulated pathway. Furthermore,...

Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by high levels of proinflammatory cytokines and epithelial barrier dysfunction. The root Ligularia fischeri (Ledeb.) Turcz. traditional Chinese medicinal herb with diverse therapeutic properties, which has been successfully used to treat inflammation-related diseases. However, little known about its effect mechanism against UC.To investigate the efficacy L. extracts UC.L. samples were prepared using alcohol...

Nanoparticles (NPs) are considered to influence the inflammatory process; however, precise mechanism and significance in tumors still not clear. In this study, when CT26 LL2 mouse cancer cells were treated with 6-nm anatase titanium dioxide NPs (TDNPs) without ultraviolet irradiation, oxidative stress induction of cytokines observed. Oxidative was further increased by disease-associated conditions such as high glucose concentrations hypoxia. Inhaled or orally administered TDNPs generated...

Osteoarthritis (OA) is one of the most common but refractory degenerative articular cartilage diseases. The emerging human amniotic mesenchymal cells (hAMSCs) have been identified a promising stem cell source in repair, and hyaluronic acid (HA) versatile regulator for transplantation. Herein, an effective straightforward intra-articular injection therapy using cocktail hAMSCs HA developed to treat knee OA rat model. injured remarkably regenerated very close level normal after 56 days...

Proton pump inhibitors (PPIs) are administered commonly to aged people; however, their effect on colorectal cancer (CRC) has still not been fully elucidated. Here, we examined the of PPIs and consequent alkalization CRC cells. PPI administration alkalized fecal pH increased serum gastrin concentration. pH8 treatment (alkalization) CMT93 mouse colon cells inhibited cell growth invasion, oxidative stress apoptosis, decreased mitochondrial volume protein levels cyclin D1 phosphorylated...

Hyperglycemia and dyslipidemia are classic features of patients with diabetes mellitus (DM). Cordyceps taii, a folk medicinal fungus native to southern China, possesses various pharmacological activities. This study aimed assess the glucose-lowering hypolipidemic effects polysaccharides from C. taii (CTP) in streptozotocin (STZ)-induced diabetic mice. Kunming mice were intraperitoneally injected STZ at dose 100 mg/kg body weight. After induction diabetes, randomly divided into five groups:...

Aging is an important risk factor in the occurrence of many chronic diseases. Senescence and exhaustion adult stem cells are considered as a hallmark aging organisms. In this study, senescent human amniotic mesenchymal cell (hAMSC) model subjected to oxidative stress was established vitro using hydrogen peroxide. We investigated effects ganoderic acid D (GA-D), natural triterpenoid compound produced from Ganoderma lucidum , on hAMSC senescence. GA-D significantly inhibited β -galactosidase...

// Chie Nakashima 1 , 2 Kazuhiko Yamamoto Shingo Kishi Takamitsu Sasaki Hitoshi Ohmori Rina Fujiwara-Tani Shiori Mori Isao Kawahara Yukiko Nishiguchi Takuya Masuo Kondoh 3 Yi Luo 4 Tadaaki Kirita and Hiroki Kuniyasu Department of Molecular Pathology, Nara Medical University, Kashihara, 634-8521, Japan Oral Maxillofacial Surgery, 634-8522, Drug Innovation Center, Graduate School Pharmaceutical Sciences, Osaka Suita, 565-0871, Key Laboratory Neuroregeneration Jiangsu Ministry Education,...

Abstract Background Gut microbiota have a complex role on the survivability, digestive physiology, production, and growth performance in animals. Recent studies emphasized effects of prebiotics therapy gut disease, but relationship between elephant gut-related diseases remains elusive. Here, case study was undertaken to evaluate mechanism inulin treatment colic Asian ( Elephas maximus Linnaeus). Methods Fecal samples were collected from sick four healthy elephants. Analysis microbial profile...

One of the challenges using imaging techniques as a tool to study cellular physiology has been inability resolve structures that are not located near surface preparation. Nonlinear optical microscopy, in particular two photon-excited fluorescence microscopy (2PM), overcome this limitation, providing deeper penetration (several hundred µm) ex vivo and preparations. We have used approach gut achieve first enteric neurons nerve fibers mucosa, submucosa, submucosal myenteric plexuses, circular...

Osteogenic inducers play central roles in effective stem cell-based treatment of bone defects/losses. However, the current routine osteogenic inducer is a cocktail comprising three components that must be improved due to low induction efficiency and side effects. Therefore, there an urgent need develop safer more osteoinducers. Herein, we demonstrated effect Ganoderal A (GD-A), tetracyclic triterpenoid compound from Ganoderma lucidum. GD-A showed no cytotoxicity toward human amniotic...

Abstract Cancer‐derived myocardial damage is an important cause of death in cancer patients. However, the development dietary interventions for treating such has not been advanced. Here, we investigated effect intervention with lauric acid (LAA) and glucose, which was effective against skeletal muscle sarcopenia a mouse cachexia model, on damage. Treatment H9c2 rat cardiomyoblasts promoted mitochondrial respiration increased ATP production by Seahorse flux analysis, but did increase...

CD44 antigen (CD44) is a transmembrane protein found in cell adhesion molecules and involved the regulation of various physiological processes cells. It was hypothesized that directly affected chondrogenic differentiation human amniotic mesenchymal stem cells (hAMSCs). In present study, expression chondrocyte‑associated factors detected absence presence antibody blocker anti‑CD44 during hAMSCs. Following inhibition expression, transcriptional levels genes SRY‑box transcription factor 9,...

Clinically, when the diagnosis of colorectal cancer is clear, patients are more concerned about their own prognosis survival. Special population with high risk accidental death, such as elderly patients, likely to die due causes other than tumors. The main purpose this study construct a prediction model cause-specific death (CSD) in using competing-risk approach, so help clinicians predict probability CSD cancer.The data were extracted from Surveillance, Epidemiology, and End Results (SEER)...

Patients with cancer die from cardiac dysfunction second only to the disease itself. Cardiotoxicity caused by anticancer drugs has been emphasized as a possible cause; however, details remain unclear. To investigate this mechanism, we treated rat cardiomyoblast H9c2 cells sunitinib, lapatinib, 5-fluorouracil, and cisplatin examine their effects. All increased ROS, lipid peroxide, iron (II) levels in mitochondria decreased glutathione peroxidase-4 GSH/GSSG ratio. Against background,...

The effect of diabetic conditions on liver metastasis was examined using CT26 mouse colon cancer cells. cells produced angiotensin (A)-I and A-II from angiotensinogen; the production abrogated by inhibitors renin chymase. Renin expression increased with an increase in concentration glucose medium. In a streptozotocin-induced BALB/c diabetes model that fed high-calorie diet, blood sugar level associated increasing size number metastases. this model, suppressed anti-angiotensin treatment...

Abstract Skeletal muscle volume is associated with prognosis of cancer patients. Maintenance skeletal an essential concern in treatment. In nutritional intervention, it important to focus on differences metabolism between tumor and muscle. We examined the influence oral intake glucose (0%, 10%, 50%) 2% medium‐chain fatty acid (lauric acid, LAA , C12:0) growth atrophy mouse peritoneal metastasis models using CT 26 colon cells HT 29 human cells. After 2 weeks experimental breeding, were...

Aims: Regenerating islet‐derived family, member 4 (Reg IV) is associated with the progression of various cancers. The aim was to examine Reg IV expression in adenoid cystic carcinomas (ACCs) salivary glands. Methods and results: detected by immunohistochemistry compared clinicopathological parameters. Expression phosphorylated epidermal growth factor receptor (pEGFR), AKT (pAKT) MUC2 examined immunohistochemistry. function assessed antisense S‐oligodeoxynucleotides (AS) ACC3 human ACC cells....