A5106722626- Cancer, Hypoxia, and Metabolism
- Barrier Structure and Function Studies
- Gastrointestinal motility and disorders
- Nutrition and Health in Aging
- Congenital gastrointestinal and neural anomalies
- Mitochondrial Function and Pathology
- Muscle Physiology and Disorders
- Fatty Acid Research and Health
- Stroke Rehabilitation and Recovery
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Vestibular and auditory disorders
- Acute Ischemic Stroke Management
- Cerebral Venous Sinus Thrombosis
- Cancer, Lipids, and Metabolism
- Hedgehog Signaling Pathway Studies
- Diet and metabolism studies
- Atrial Fibrillation Management and Outcomes
- Neurological Disease Mechanisms and Treatments
- Muscle metabolism and nutrition
- Enhanced Recovery After Surgery
- Gastric Cancer Management and Outcomes
- Dementia and Cognitive Impairment Research
- Cardiovascular Function and Risk Factors
- Hearing, Cochlea, Tinnitus, Genetics
Nara Medical University
2015-2025
Nippon Steel Yawata Memorial Hospital
2021-2024
Nagano Municipal Hospital
2024
Nara Medical University Hospital
2019
Niigata University
2013-2018
Niigata University Medical and Dental Hospital
2015-2017
National Hospital Organization
2017
Kokura Memorial Hospital
2015
University of Occupational and Environmental Health Japan
2006-2014
Anzai (Japan)
2012-2013
Cancer dormancy is a state characterized by the quiescence of disseminated cancer cells, and tumor recurrence occurs when such cells re-proliferate after long incubation period. These tend to be treatment resistant one barriers successful therapeutic intervention. We have previously reported that long-term with linoleic acid (LA) induces dormancy-like phenotype. However, mechanism underpinning this effect has not yet been clarified. Here, we investigate LA-induced in cells. first confirmed...
// Rina Fujiwara-Tani 1 , Takamitsu Sasaki Yi Luo 1, 2 Kei Goto Isao Kawahara Yukiko Nishiguchi Shingo Kishi Shiori Mori Hitoshi Ohmori Masuo Kondoh 3 and Hiroki Kuniyasu Department of Molecular Pathology, Nara Medical University, Kashihara, 634-8521, Japan Jiangsu Province Key Laboratory Neuroregeneration, Nantong Nantong, 226001, China Drug Innovation Center, Graduate School Pharmaceutical Sciences, Osaka Suita, 565-0871, Correspondence to: Kuniyasu, email: cooninh@zb4.so-net.ne.jp...
CD47 activates signal regulatory protein alpha expressed on macrophages and suppresses its phagocytic ability; therefore, is drawing attention as an immune checkpoint in the innate system. Expression of cancer thought to allow cells escape antitumor immunity In this study, expression was examined by immunostaining colorectal (CRC) compared with CD44, which a marker for stem cells. 95 cases stage II–IV CRC, CD44 showed overexpression 82 80 cases, respectively. Both levels correlated distant...
Abstract Triple negative breast cancer (TNBC) is characterized by highly aggressive phenotype, limited treatment options and a poor prognosis. In the present study, we examined therapeutic effect of anti–claudin (CLDN)‐4 extracellular domain antibody, 4D3, on TNBC. When expression CLDN4 CLDN1 in invasive ductal carcinoma (IDC) was 114 IDC (78 cases from 2004 to 2009 single center 36 tissues array), had lower than correlated with histological grade. contrast, grade, receptor subtype, stage....
One of the challenges using imaging techniques as a tool to study cellular physiology has been inability resolve structures that are not located near surface preparation. Nonlinear optical microscopy, in particular two photon-excited fluorescence microscopy (2PM), overcome this limitation, providing deeper penetration (several hundred µm) ex vivo and preparations. We have used approach gut achieve first enteric neurons nerve fibers mucosa, submucosa, submucosal myenteric plexuses, circular...
Oral intake of medium-chain fatty acids (MCFAs) reportedly suppresses the accumulation visceral fat and has antitumor effects in tumor-bearing animals. MCFAs penetrate mitochondrial membrane a carnitine shuttle-independent manner are metabolized more quickly than long-chain acids. Based on these characteristics, may have pronounced mitochondria-rich tissues, such as myocardium. We examined effect oral heart.We fed BALB/c mice with control diet supplemented 0%, 2%, 5%, or 10% lauric acid...
// Yukiko Nishiguchi 1, 2 , Rina Fujiwara-Tani 1 Takamitsu Sasaki Yi Luo 3 Hitoshi Ohmori Shingo Kishi Shiori Mori Kei Goto Wataru Yasui 4 Masayuki Sho and Hiroki Kuniyasu Department of Molecular Pathology, Nara Medical University, Kashihara, 634-8521, Japan Surgery, 634-8522, Jiangsu Province Key Laboratory Neuroregeneration, Nantong Nantong, 226001, China Hiroshima University Graduate School, 734-8551, Correspondence to: Kuniyasu, email: cooninh@zb4.so-net.ne.jp Sho, m-sho@naramed-u.ac.jp...
Abstract Cancer‐derived myocardial damage is an important cause of death in cancer patients. However, the development dietary interventions for treating such has not been advanced. Here, we investigated effect intervention with lauric acid (LAA) and glucose, which was effective against skeletal muscle sarcopenia a mouse cachexia model, on damage. Treatment H9c2 rat cardiomyoblasts promoted mitochondrial respiration increased ATP production by Seahorse flux analysis, but did increase...
An orally administered serotonin-4 (5-HT4) receptor agonist, mosapride citrate (MOS), promotes enteric neurogenesis in anastomoses after gut surgery. We performed surgery and transplanted 2 × 105 neural stem cells (NSCs) from the embryonic central nervous system marking them with cell linker, PKH26. found that neurons differentiated NSCs (PKH [+]) newborn mobilized (host) (YFP deep granulation tissue of anastomotic ileum. MOS significantly increased number PKH (+) YFP by 2.5-fold (P < 0.005)...
Abstract Skeletal muscle volume is associated with prognosis of cancer patients. Maintenance skeletal an essential concern in treatment. In nutritional intervention, it important to focus on differences metabolism between tumor and muscle. We examined the influence oral intake glucose (0%, 10%, 50%) 2% medium‐chain fatty acid (lauric acid, LAA , C12:0) growth atrophy mouse peritoneal metastasis models using CT 26 colon cells HT 29 human cells. After 2 weeks experimental breeding, were...
Abstract Claudin (CLDN) family comprises of protein that form a tight junction, and is involved in regulating polarity differentiation cells. Here, we aimed to investigate the effects inhibiting CLDN4 pancreatic ductal carcinomas (PDC). We first examined 91 cases human PDC by immunohistochemistry found expression was correlated with tumor invasion, nodal metastasis, distant metastasis. Anti‐CLDN4 extracellular domain antibody, previously established us (4D3), inhibited proliferation...
Budding at the tumor invasive front has been correlated with malignant properties of many cancers. Malic enzyme 1 (ME1) promotes Warburg effect in cancer cells and induces epithelial–mesenchymal transition (EMT) oral squamous cell carcinoma (OSCC). Therefore, we investigated role ME1 budding OSCC. Tumor was measured 96 human OSCCs by immunostaining for an epithelial marker (AE1/AE3), its expression compared that ME1. A significant correlation observed between expression. The increased...
Claudin-4 (CLDN4) is a tight junction protein to maintain the cancer microenvironment. We recently reported role of CLDN4 not forming in induction epithelial-mesenchymal transition (EMT). Herein, we investigated renal cell carcinoma (RCC), focusing on CLDN4. expression 202 RCCs was examined by immunostaining. phosphorylation and subcellular localization were using high metastatic human RCC SN12L1 low SN12C lines. In cases, decreased membrane had no correlation with clinicopathological...
It was recently reported that activation of enteric neural 5-HT(4) receptors (SR4) promotes reconstruction circuit injury in distal gut guinea pigs and this involves stem cells. We aimed to explore a novel approach using selective serotonin reuptake inhibitor (SSRI), which increases endogenous 5-HT, repair nerve fiber the rat gut. Enteric performed by rectal transection subsequent end-to-end one-layer anastomosis. The SSRI fluvoxamine maleate (100 μmol/l) applied locally at anastomotic site...
The effect of intermittent calorie restriction (ICR) on cancer is controversial. In this study, we examined the effects ICR and food content in syngeneic BALB/c mice injected with CT26 mouse colon cells. Mice were subjected to 24-h fasting once a week for 4 weeks, then provided control, high-calorie, or trans fatty acid-rich diet. While resulted increases tumor weights, metastasis number stem cells (CSCs) tumors blood fed control high-fat diets, it had no body weight after weeks. particular,...
An increased risk of colorectal cancer (CRC) is associated with a western style diet, particularly hyperlipidemia. The expression G-protein coupled receptor 40 (GPR40), membrane-bound for long-chain fatty acids (LCFAs), was examined in 36 cases subserosal-invading CRC and compared clinicopathological parameters as well triglyceride (TG) low-density lipoprotein (LDL) levels the blood. All patients expressed GPR40, which positively blood TG (P<0.0001) but not LDL levels. GPR40 nodal...
Cachexia frequently occurs in cancer patients and is correlated with reduced therapeutic responsiveness poor prognosis. Although skeletal muscle atrophy an important factor related to cachexia, biomarkers for its early diagnosis are not yet definitive. In this study, weight loss, body mass index, index (SMI), serum carcinoembryonic antigen, tumor necrosis (TNF)-α, interleukin (IL)-6, high mobility group box (HMGB)-1, SDS-soluble myosin light chain 1 (SDS-MYL1) of the psoas were examined 8...
// Rina Fujiwara-Tani 1 , Takamitsu Sasaki Kiyomu Fujii Yi Luo 2 Takuya Mori Shingo Kishi Shiori Sayako Matsushima-Otsuka Yukiko Nishiguchi Kei Goto Isao Kawahara Masuo Kondoh 3 Masayuki Sho 4 and Hiroki Kuniyasu Department of Molecular Pathology, Nara Medical University, Kashihara, 634-8521, Japan Key Laboratory Neuroregeneration Jiangsu Ministry Education, Co-Innovation Center Neuroregeneration, Nantong Nantong, Province 226001, China Drug Innovation Center, Graduate School Pharmaceutical...
β-Casomorphin-7 (BCM) is a degradation product of β-casein, milk component, and has been suggested to affect the immune system. However, its effect on mucosal immunity, especially anti-tumor in cancer-bearing individuals not clear. We investigated effects BCM lymphocytes using an vitro system comprising mouse splenocytes, colorectal carcinogenesis model, orthotopic cancer model. Treatment splenocytes with reduced numbers cluster differentiation (CD) 20+ B cells, CD4+ T regulatory cells...
Advanced glycation end products (AGEs) are produced in response to a high-glucose environment and oxidative stress exacerbate various diseases.