A5035040549- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Ruminant Nutrition and Digestive Physiology
- Genetic Mapping and Diversity in Plants and Animals
- Meat and Animal Product Quality
- Gut microbiota and health
- Muscle Physiology and Disorders
- Agriculture Sustainability and Environmental Impact
- Circular RNAs in diseases
- Genetic Syndromes and Imprinting
- Aquaculture Nutrition and Growth
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Peroxisome Proliferator-Activated Receptors
- Selenium in Biological Systems
- RNA Research and Splicing
- RNA modifications and cancer
- Effects of Environmental Stressors on Livestock
- Animal Nutrition and Physiology
- Lipid metabolism and disorders
- Aquaculture disease management and microbiota
- Fatty Acid Research and Health
- Nuts composition and effects
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
Brazilian Agricultural Research Corporation
2016-2024
Universidade Federal de São Carlos
2011-2024
University of Coimbra
2015
Universidade Estadual Paulista (Unesp)
2010
Intramuscular fat (IMF) content is related to insulin resistance, which an important prediction factor for disorders, such as cardiovascular disease, obesity and type 2 diabetes in human. At the same time, it economically trait, influences sensorial nutritional value of meat. The deposition IMF influenced by many factors sex, age, nutrition, genetics. In this study Nellore steers (Bos taurus indicus subspecies) were used better understand molecular mechanisms involved content. This was...
Abstract Residual feed intake (RFI), a measure of efficiency (FE), is defined as the difference between observed and predictable considering size growth animal. It extremely important to beef production systems due its impact on allocation land areas alternative agricultural production, animal methane emissions, food demand cost production. Global differential gene expression analysis high low RFI groups (HRFI LRFI: less more efficient, respectively) revealed 73 differentially expressed (DE)...
Lipids are a class of molecules that play an important role in cellular structure and metabolism all cell types. In the last few decades, it has been reported long-chain fatty acids (FAs) involved several biological functions from transcriptional regulation to physiological processes. Several have both positively negatively implicated different processes skeletal muscle other tissues. To gain insight into associated with acid content muscle, aim present study was identify differentially...
Integration of high throughput DNA genotyping and RNA-sequencing data allows for the identification genomic regions that control gene expression, known as expression quantitative trait loci (eQTL), on a whole genome scale. Intramuscular fat (IMF) content carcass composition play important roles in metabolic physiological processes mammals because they influence insulin sensitivity consequently prevalence diseases such obesity type 2 diabetes. However, limited information is available genetic...
Nelore is the major beef cattle breed in Brazil with more than 130 million heads. Genome-wide association studies (GWAS) are often used to associate markers and genomic regions growth meat quality traits that can be assist selection programs. An alternative methodology traditional GWAS involves construction of gene network interactions, derived from results several AWM (Association Weight Matrices)/PCIT (Partial Correlation Information Theory). With aim evaluating genetic architecture...
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are important dimension post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression weighted gene co-expression network analyses (WGCNA) to better understand complex interactions between mRNAs expressed bovine skeletal muscle liver. MiRNA mRNA data were obtained from Nelore...
Feed efficiency helps to reduce environmental impacts from livestock production, improving beef cattle profitability. We identified potential biomarkers (hub genes) for feed efficiency, by applying co-expression analysis in Longissimus thoracis RNA-Seq data 180 Nelore steers. Six modules were associated with six efficiency-related traits (p-value ≤ 0.05). Within these modules, 391 hub genes enriched pathways as protein synthesis, muscle growth, and immune response. Trait-associated...
The success of different species ruminants in the colonization a diverse range environments is due to their ability digest and absorb nutrients from cellulose, complex polysaccharide found leaves grass. Ruminants rely on microbial community, or microbiota, unique compartment known as rumen break down this polysaccharide. Changes populations can affect host's development, health, productivity. However, accessing stressful for animal. Therefore, development use alternative sampling methods are...
Background: The impact of extreme changes in weather patterns on the economy and human welfare is one biggest challenges our civilization faces. From anthropogenic contributions to climate change, reducing farming activities a priority since it responsible for up 18% global greenhouse gas emissions. To this end, we tested whether ruminal stool microbiome components could be used as biomarkers methane emission feed efficiency bovine by studying 52 Brazilian Nelore bulls belonging two...
Meat quality is a complex trait that influenced by genetic and environmental factors, which includes mineral concentration. However, the association between concentration meat quality, specific molecular pathways underlying this association, are not well explored. We therefore analyzed gene expression as measured with RNA-seq in Longissimus thoracis muscle of 194 Nelore steers for three traits (intramuscular fat, pH, tenderness) 13 minerals (Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, P, S, Se, Zn)....
Iron (Fe) is an essential mineral for metabolism and plays a central role in range of biochemical processes. Therefore, this study aimed to identify differentially expressed (DE) genes metabolic pathways Longissimus dorsi (LD) muscle from cattle with divergent iron content, as well investigate the likely these DE biological processes underlying beef quality parameters. Samples RNA extraction sequencing iron, copper, manganese, zinc determination were collected LD muscles at slaughter. Eight...
Fatty acid (FA) content affects the sensorial and nutritional value of meat plays a significant role in biological processes such as adipogenesis immune response. It is well known that, beef, main FAs associated with these are oleic (C18:1 cis9, OA) conjugated linoleic (CLA-c9t11), which may have beneficial effects on metabolic diseases type 2 diabetes obesity. Here, we performed differential expression co-expression analyses, weighted gene network analysis (WGCNA) partial correlation...
MicroRNAs (miRNAs) are key regulators of gene expression, potentially affecting several biological processes, whose function can be altered by sequence variation. Hence, the integration single nucleotide polymorphisms (SNP) and miRNAs explain individual differences in economic traits. To provide new insights into effects SNPs on their related target genes, we carried out a multi-omic analysis to identify miRNA mature sequences (miR-SNPs) associated with fatty acid (FA) composition Nelore...
Abstract Differences between the expression of two alleles a gene are known as allele-specific (ASE), common event in transcriptome mammals. Despite ASE being source phenotypic variation, its occurrence and effects on genetic prediction economically relevant traits still unexplored bovines. Furthermore, events likely driven by cis-regulatory mutations, scanning them throughout bovine genome represents significant step to elucidate mechanisms underlying regulation. To address this question...
Abstract Background Beef tenderness is a complex trait of economic importance for the beef industry. Understanding epigenetic mechanisms underlying this may help improve accuracy breeding programs. However, little known about effects on Bos taurus muscle and their implications in tenderness, no studies have been conducted indicus . Results Comparing methylation profile skeletal with contrasting at 14 days after slaughter, we identified differentially methylated cytosines regions associated...
Abstract Feed cost represents a major economic determinant within cattle production, amounting to an estimated 75% of the total variable costs. Consequently, comprehensive approaches such as optimizing feed utilization through alternative sources, alongside selection feed-efficient animals, are great significance. Here, we investigate effect two diets, traditional corn-grain fed and by-product based, on 14 phenotypes related feed, methane emission production efficiency multi-tissue...
Seis ovinos machos, não castrados, da raça Santa Inês, com média de peso 30kg, fistulados no rúmen, foram distribuídos em delineamento quadrado-latino duplo (3x3). Três períodos e três dietas, uma controle, sem inclusão fonte lipídio, duas grãos girassol ou gordura protegida, testados quanto aos parâmetros ruminais. Foram verificadas diferenças (P<0,05) entre as dietas à concentração ruminal amônia (18mg/dL), mas houve efeito sobre o pH (6,1), a produção total ácidos graxos cadeia curta...
Background Ruminants harbor a complex microbial community within their gastrointestinal tract, which plays major roles in health and physiology. Brazil is one of the largest producers beef world more than 90% cattle herds are composed pure crossbred Nelore ( Bos indicus ). Despite its importance to Brazilian economy human feeding, few studies have characterized microbiome. Therefore, using shotgun metagenomics, we investigated impact diet on composition functionality microbiome, explored...
Abstract Nelore is the most economically important cattle breed in Brazil, and use of genetically improved animals has contributed to increased beef production efficiency. The Brazilian feedlot industry grown considerably last decade, so selection with higher growth rates on become quite important. Genomic (GS) could be used reduce generation intervals improve rate genetic gains. aim this study was evaluate prediction genomic-estimated breeding values (GEBV) for average daily weight gain...
In contrast to the Mendelian inheritance model, parental alleles can contribute unequally gene expression, which may result in phenotypic variance among individuals and bias predicted additive effect of molecular markers associated with production traits.Given need understand effects allelic variation parentof-origin on expression genes a commercial interest cattle, we analyzed KCNJ11 (potassium inwardly Genetics Molecular Research 15 (3): gmr.15038549 rectifying channel, subfamily J, member...
Residual feed intake (RFI) can increase the profitability of producers, reduce methane emission and land allocation to livestock production. However, this trait has late costly measurements. Identifying gene expression changes combined with polymorphisms that affect residual variation is important for identify target regulatory be used in animal breeding programs. Diverse studies performed by our research group a Nellore population, such as genome-wide association (GWA), weight matrix (AWM)...