A5061421513- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Metal-Organic Frameworks: Synthesis and Applications
Université Paris Cité
2021-2025
Institut de Biologie Physico-Chimique
2021-2025
Centre National de la Recherche Scientifique
2021-2025
Expression Génétique Microbienne
2021-2024
Abstract The human mitochondrial genome is transcribed into two RNAs, containing mRNAs, rRNAs and tRNAs, all dedicated to produce essential proteins of the respiratory chain. precise excision tRNAs by endoribonucleases (mt-RNase), P Z, releases RNA species from transcripts. then undergo 3′-CCA addition. In metazoan mitochondria, RNase a multi-enzyme assembly that comprises endoribonuclease PRORP tRNA methyltransferase subcomplex. requirement for this subcomplex mt-RNase cleavage activity, as...
ConspectusRNA modification is a co- or post-transcriptional process by which specific nucleotides are chemically altered enzymes after their initial incorporation into the RNA chain, expanding chemical and functional diversity of RNAs. Our understanding modifications has changed dramatically in recent years. In past decade, methyltransferases (MTases) have been highlighted numerous clinical studies disease models, found to be dynamically regulated demodification enzymes, significant...
Chemical synthesis of RNA conjugates has opened new strategies to study enzymatic mechanisms in biology. To gain insights into poorly understood nucleotide methylation processes, we developed a method synthesize RNA-conjugates for the recognition and methyl-transfer SAM-dependent m6A methyltransferases. These contain SAM cofactor analogue connected at N6-atom an adenosine within dinucleotides, trinucleotide or 13mer RNA. Our chemical route is chemo- regio-selective allows flexible...
Abstract The human mitochondrial genome is transcribed into two RNAs, containing mRNAs, rRNAs and tRNAs, all dedicated to produce essential proteins of the respiratory chain. precise excision tRNAs by endoribonucleases (mt-RNase), mt-RNase P Z, releases RNA species from transcripts. then undergo 3’-CCA addition. In metazoan mitochondria, RNase a multi-enzyme assembly that comprises endoribonuclease PRORP tRNA methyltransferase subcomplex. requirement for complex in cleavage activity not...