A5063564062- RNA modifications and cancer
- Cancer-related gene regulation
- Chemical Reactions and Isotopes
- RNA and protein synthesis mechanisms
- Microbial Metabolites in Food Biotechnology
- Mass Spectrometry Techniques and Applications
- Epigenetics and DNA Methylation
- Fluorine in Organic Chemistry
- Amino Acid Enzymes and Metabolism
- Cancer-related molecular mechanisms research
- Metabolism and Genetic Disorders
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2018-2022
Université Paris Cité
2018-2022
Centre National de la Recherche Scientifique
2019-2022
RNA methyltransferases (MTases) catalyse the transfer of a methyl group to their substrates using most-often S-adenosyl-L-methionine (SAM) as cofactor. Only few RNA-bound MTases structures are currently available due difficulties in crystallising RNA:protein complexes. The lack complex results poorly understood recognition patterns and methylation reaction mechanisms. On contrary, many cofactor-bound MTase available, resulting well-understood protein:cofactor recognition, that can guide...
Chemical synthesis of RNA conjugates has opened new strategies to study enzymatic mechanisms in biology. To gain insights into poorly understood nucleotide methylation processes, we developed a method synthesize RNA-conjugates for the recognition and methyl-transfer SAM-dependent m6A methyltransferases. These contain SAM cofactor analogue connected at N6-atom an adenosine within dinucleotides, trinucleotide or 13mer RNA. Our chemical route is chemo- regio-selective allows flexible...
RNA methyltransferases (RNMTs) catalyze the methylation of using S‐adenosyl‐ l ‐methionine (SAM) as methyl donor. Methylation at N‐6 position adenosine is most abundant modification found in nearly all classes RNAs and contributes to regulation many biological processes three domains life. However, this family enzymes remains relatively unexplored by medicinal chemistry community new molecules are needed for their studies. Since RNMTs suitable bisubstrate binding, we report here synthesis...
More than 150 RNA chemical modifications have been identified to date. Among them, methylation of adenosine at the N-6 position (m6A) is crucial for metabolism, stability and other important biological events. In particular, this most abundant mark found in mRNA mammalian cells. The presence a methyl group N-1 (m1A) mostly ncRNA mainly responsible translation fidelity. These are installed by m6A m1A methyltransferases (RNA MTases), respectively. human, deregulation MTases activity associated...