A5008360063- Biochemical and Molecular Research
- DNA and Nucleic Acid Chemistry
- HIV/AIDS drug development and treatment
- Synthesis and Characterization of Heterocyclic Compounds
- Cytomegalovirus and herpesvirus research
- Synthesis and Biological Evaluation
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Hepatitis C virus research
- Synthesis and biological activity
- Virus-based gene therapy research
- Advanced biosensing and bioanalysis techniques
- Inorganic and Organometallic Chemistry
- Hepatitis B Virus Studies
- Synthesis and characterization of novel inorganic/organometallic compounds
- Metal complexes synthesis and properties
- Quinazolinone synthesis and applications
- Cardiac, Anesthesia and Surgical Outcomes
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Chemical Synthesis and Analysis
- Acute Ischemic Stroke Management
- Polyomavirus and related diseases
- Synthesis of heterocyclic compounds
- Mollusks and Parasites Studies
Valley Baptist Medical Center
2024
Higher Colleges of Technology
2024
Zagazig University
2013-2023
Mayo Clinic
2023
Mayo Clinic in Arizona
2022
North Cumbria Integrated Care NHS Foundation Trust
2022
Southern Research Institute
2000-2020
South Valley University
2020
Brookhaven National Laboratory
2012
Georgia State University
2008-2012
The base pairs are the contributors to sequence-dependent recognition of nucleic acids, genetic information storage, and high fidelity DNA polymerase replication. However, wobble pairing, where T with G instead A, reduces specific base-pairing compromises enzymatic polymerization. Via selenium atomic probing at 2-position thymidine, we have investigated discrimination by manipulating steric electronic effects 2-exo position, providing a unique chemical strategy enhance pair specificity. We...
Specificity of nucleobase pairing provides essential foundation for genetic information storage, replication, transcription and translation in all living organisms. However, the wobble base pairs, where U RNA (or T DNA) pairs with G instead A, might compromise high specificity pairing. The U/G is ubiquitous RNA, especially non-coding RNA. In order to increase U/A specificity, we have hypothesized discriminate against pair by tailoring steric electronic effects at 2-exo position uridine...
ABSTRACT Treatment with antimetabolites results in chemically induced low nucleoside triphosphate pools and cell cycle arrest exponentially growing cells. Since steady-state levels of hepatitis C virus (HCV) replicon RNA were shown to be dependent on exponential growth Huh-7 cells, the effects for several biosynthesis pathways HCV investigated. A specific anti-HCV effect was defined as (i) minimal interference growth, (ii) reduction cellular host levels, (iii) copy number per compared that...
We investigated the possibility of interaction 5-CH3 thymidine and its 5′-phosphate backbone (C−H···O−−PO3 interaction) in DNA via insertion atomic probe (a selenium atom) into exo-5-position (5-Se-T). 5-Se-T was synthesized for first time, Mn(OAc)3 assisted electrophilic addition CH3SeSeCH3 to 3′,5′-di-O-benzoyl-2′-deoxyuridine. The phosphoramidite subsequently incorporated over 99% coupling yield. Biophysical structural investigations DNAs revealed that Se-modified nonmodified are...
The selected nicotinamide-based HDAC<italic>i</italic> displayed selectivity towards HDAC3 over pan HDAC and exhibited potent cytotoxicity against the used cell lines.
We report here the first synthesis of 5-phenyl–telluride–thymidine derivatives and Te-phosphoramidite. also synthesis, structure STM current-imaging studies DNA oligonucleotides containing nucleobases (thymine) derivatized with 5-phenyl-telluride functionality (5-Te). Our results show that 5-Te-DNA is stable, Te-DNA duplex has thermo-stability similar to corresponding native duplex. The crystal indicates virtually identical one, Te-modified T A interact similarly pair. Furthermore, while...
We report here the first synthesis of Te-nucleoside phosphoramidites and Te-modified oligonucleotides. protected 2'-tellurium functionality by alkylation found that Te is compatible with solid-phase oligonucleotides are stable during deprotection purification. In addition, redox properties functionalities have been explored. telluride telluoxide DNAs interchangeable reactions. At elevated temperature, Te-DNA can also be site-specifically fragmented oxidatively or reductively when 2'-TePh...
Natural RNAs, especially tRNAs, are extensively modified to tailor structure and function diversities. Uracil is the most nucleobase among all natural nucleobases. Interestingly, >76% of uracil modifications located on its 5-position. We have investigated 5-methoxy (5-O-CH3) modification in context A-form oligonucleotide duplex. Our X-ray crystal indicates first a H-bond formation between 5-O-CH3 5′-phosphate. This novel not observed when oxygen replaced with larger atom (selenium or...
Several 2',3'-dideoxynucleosides (ddNs) and 2',3'-didehydro-2',3'-dideoxynucleosides (d4Ns) are FDA-approved anti-HIV drugs. Via conveniently synthesized 2,2'-anhydronucleosides, we have developed a novel synthesis of d4Ns by discovering applying new telluride-mediated elimination reaction. Our experiment results show that after substitution 2,2'-anhydronucleosides with telluride monoanion, intermediate is formed, its leads to formation the olefin products (d4Ns). mechanistic study indicates...
In this work, electrospinning technique has been used to produce a new ultra‐fine hybrid fiber from solution consists of polystyrene (PS) and epoxy polymers in dimethylformamide (DMF) solvent. Five ratios different weight percentages PS/DMF solutions 4 concentrations; 15, 20, 25 30 wt.% were made obtain the minimum diameter produced fiber. The obtained was found be 897 ± 239 nm at PS/epoxy ratio 5:1 13.33 Berry's number. Adding PS reduced electrospun 2.64 0.49 μm for only 1.52 0.72 fibers...
The synthesis of a novel series 4-arylhydrazono-5-methyl-1,2-dihydropyrazol-3-ones 4a-h, and their N (2)-alkyl acyclo, glucopyranosyl, ribofuranosyl derivatives is described. K(2)CO(3) catalyzed alkylation 4a-h with allyl bromide, propargyl 4-bromobutyl acetate, 2-acetoxyethoxymethyl 2,3,4,6-tetra-O-acetyl-α-D-glucopyranosyl bromide proceeded selectively at the (2)-position pyrazolinone ring. Glycosylation 4a 1,2,3,5-tetra-O-acetyl-β-D-ribofuranose under Vorbruggen glycosylation conditions...
Trimethylsilyl-transient protection successfully allowed the use of lithium hexamethyldisilazane to prepare benzimidazole (BI) and 4-azabenzimidazole (azaBI) amidines from nitriles in 58–88% yields. This strategy offers a much better choice BI/azaBI than lengthy, low-yielding Pinner reaction. Synthesis aza/benzimidazole rings aromatic diamines aldehydes was affected dimethyl sulfoxide 10–15 min, while known procedures require long time purification. These methods are important for...
Abstract Efficient methods for the synthesis of 6-methylpurine (3), 9-(2-deoxy-β-D-erythro-pentofuranosyl)-6-methylpurine (8), and 6-methyl-9-β-D-ribofuranosylpurine (5) are described. Methodology involving (Ph3P)4Pd catalyzed cross-coupling reaction CH3ZnBr with several different 6-chloropurine derivatives is described in high yield. This methodology now provides a facile high-yielding 8, which needed significant amounts studies cancer gene therapy.