A5049300063- Solar and Space Plasma Dynamics
- Calibration and Measurement Techniques
- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Astro and Planetary Science
- Stellar, planetary, and galactic studies
- Atmospheric Ozone and Climate
- Optical Coherence Tomography Applications
- Erythrocyte Function and Pathophysiology
- Hemoglobinopathies and Related Disorders
- Analytical Chemistry and Sensors
- Astrophysical Phenomena and Observations
- Planetary Science and Exploration
- Hippo pathway signaling and YAP/TAZ
- Near-Field Optical Microscopy
- Electrochemical sensors and biosensors
- Geomagnetism and Paleomagnetism Studies
- Spacecraft and Cryogenic Technologies
- Water Quality Monitoring and Analysis
- Nuclear Physics and Applications
- Image Processing Techniques and Applications
- Spacecraft Design and Technology
- Photocathodes and Microchannel Plates
- Oil, Gas, and Environmental Issues
- Plasma Diagnostics and Applications
Marshall Space Flight Center
2020-2024
Universities Space Research Association
2018-2022
University of Notre Dame
2014-2018
Bellevue Hospital Center
2013
Intellectual Ventures (United States)
2013
The functions of the paralogous transcriptional coactivators Yes-associated protein (YAP) and coactivator with PDZ-binding motif (TAZ) in bone are controversial. Each has been observed to promote or inhibit osteogenesis vitro, reports both equivalent divergent functions. Their combinatorial roles physiology unknown. We report that YAP/TAZ deletion from skeletal lineage cells, using Osterix-Cre, caused an imperfecta-like phenotype severity dependent on allele dose greater phenotypic...
A novel space experiment achieves an unprecedented map of solar magnetic fields from the photosphere to base corona.
Abstract The third flight of the High-Resolution Coronal Imager (Hi-C 2.1) occurred on May 29, 2018; Sounding Rocket was launched from White Sands Missile Range in New Mexico. instrument has been modified its original configuration 1) to observe solar corona a passband that peaks near 172 Å, and uses new, custom-built low-noise camera. targeted Active Region 12712, captured 78 images at cadence 4.4 s (18:56:22 – 19:01:57 UT; 5 min 35 observing time). image spatial resolution varies due...
Abstract We report the first detection of a magnetic discontinuity in upper solar chromosphere associated with coronal loop brightening. Our findings are based on novel observations obtained by Chromospheric LAyer SpectroPolarimeter (CLASP2.1) suborbital space experiment. During its short 6 minute flight, CLASP2.1 successfully spectropolarimetric data across Mg ii h and k lines, which allowed us to map longitudinal field at multiple heights active region. our observation, we found...
Abstract The CLASP2 (Chromospheric LAyer Spectro-Polarimeter 2) sounding rocket mission was launched on 2019 April 11. measured the four Stokes parameters of Mg ii h and k spectral region around 2800 Å along a 200″ slit at three locations solar disk, achieving first spatially spectrally resolved observations polarization in this near-ultraviolet region. focus work presented here is center-to-limb variation linear across these resonance lines, which produced by scattering anisotropic...
The Marshall Grazing Incidence X-ray Spectrometer (MaGIXS) sounding rocket experiment launched on July 30, 2021 from the White Sands Missile Range in New Mexico. MaGIXS is a unique solar observing telescope developed to capture spectral images, 6 - 24 Angstrom wavelength range, of coronal active regions. Its novel design takes advantage recent technological advances related fabricating and optimizing optical systems as well breakthroughs inversion methodologies necessary create spectrally...
Multiphoton microscopy (MPM) combined with fluorescence lifetime imaging (FLIM) has enabled three-dimensional quantitative molecular in vivo. The signal-to-noise ratio (SNR), and thus the rate of MPM-FLIM, which is fundamentally limited by shot noise saturation, not been quantitatively studied yet. In this paper, we investigate SNR performance frequency-domain (FD) MPM-FLIM two figures merit: photon economy limit noise, normalized saturation. theoretical results Monte Carlo simulations find...
The Marshall Grazing Incidence X-ray Spectrometer (MaGIXS) is a sounding rocket instrument that flew on July 30, 2021 from the White Sands Missile Range, NM. was designed to address specific science questions require differential emission measures of solar soft spectrum 6 – 25[Formula: see text]Å(0.5 2.1[Formula: text]keV). MaGIXS comprises Wolter-I telescope, slit-jaw imaging system, an identical pair grazing incidence paraboloid mirrors, planar grating and CCD camera. While implementing...
Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit.However, due difficult implementation and high cost, super-resolution not feasible many applications.In this paper, we propose demonstrate a saturation-based technique that can be easily implemented requires neither additional hardware nor complex post-processing.The method based on principle of stepwise optical saturation (SOS), where M...
Abstract Nanoflares are thought to be one of the prime candidates that can heat solar corona its multimillion kelvin temperature. Individual nanoflares difficult detect with present generation instruments, but their presence inferred by comparing simulated nanoflare-heated plasma emissions observed emission. Using HYDRAD coronal loop simulations, we model emission from an X-ray bright point (XBP) Marshall Grazing Incidence Spectrometer (MaGIXS), along nearest available observations...
We present a series of experiments that demonstrate super-sensitive chemical imaging technique based on multiphoton frequency-domain fluorescence lifetime microscopy (MPM-FD-FLIM) shows 2× improvement in speed compared to the theoretical limit conventional MPM-FD-FLIM. Additionally, this produces unprecedented sensitivity over large range lifetimes. These results are achieved through simple modifications data analysis MPM-FD-FLIM microscope and an analytical model describing signal-to-noise...
We propose and demonstrate a dark-field imaging technique capable of automated identification individual bacteria. An 87-channel multispectral system angular spectral resolution was used to measure the scattering spectrum various bacteria in culture smears. Spectra were compared between species preparations same species. A 15-channel then prove viability bacterial with relatively simple microscope system. classifier able identify four six greater than 90% accuracy bacteria-by-bacteria testing.
Fluorophore saturation is the key factor limiting speed and excitation range of fluorescence lifetime imaging microscopy (FLIM). For example, fluorophore causes incorrect measurements when using conventional frequency-domain FLIM at high powers. In this Letter, we present an analytical theoretical description error a method for compensating in order to extract correct limit saturation. We perform series simulations experiments validate our methods. The show 13.2× 2.6× increase range,...
Abstract The Marshall Grazing Incidence Spectrometer (MaGIXS) is a sounding rocket experiment that will observe the soft X-ray spectrum of Sun from 24 to 6.0 Å (0.5–2.0 keV) and scheduled for launch in 2021. Component- instrument-level calibrations MaGIXS instrument are carried out using Cryogenic Facility (XRCF) at NASA Space Flight Center. In this paper, we present calibration incident flux electron impact source with different targets XRCF CCD camera; photon was low enough enable its use...
A wide-bandwidth, highly efficient method of inter-chip waveguide coupling suitable for on-chip, mid-infrared sensing is discussed. Simulations and preliminary fabrication work on laser-to-waveguide are presented, with losses predicted to be better than 6 dB.
Multiphoton microscopy (MPM) allows for three-dimensional in vivo scattering tissue with submicron resolution and high signal-to-noise ratio. MPM combined fluorescence lifetime measurements further enables quantitative imaging of molecular concentrations, such as dissolved oxygen, the same optical MPM, vivo. However, biocompatible oxygen-sensitive probes are not available commercially difficult to synthesize. Here we present a simple oxygen probe compatible aqueous biological media based on...
ABSTRACT The functions of the transcriptional co-activators YAP and TAZ in bone are controversial. Each has been observed to either promote or inhibit osteogenesis vitro , while their roles development unknown. Here we report that combinatorial YAP/TAZ deletion from skeletal cells mice caused imperfecta with severity dependent on targeted cell lineage allele dosage. Osteocyte-conditional impaired accrual matrix collagen, dosage-dependent all osteogenic spontaneous fractures, neonatal...
The photophysical properties of human sickle cell disease (SCD) Hemoglobin (Hb) is characterized by multi-photon microscopy (MPM). intrinsic two-photon excited fluorescence (TPEF) signal associated with extracted hemoglobin was investigated and the solidified SCD variant (HbS) found to demonstrate broad emission peaking around 510 nm when at 800 nm. MPM used dynamically induce image HbS gelling photolysis deoxygenated HbS. For comparison, conditions were applied a healthy (HbA) remain in...
We present our study on compact, label-free dissolved lipid sensing by combining capillary electrophoresis separation in a PDMS microfluidic chip online with mid-infrared (MIR) absorption spectroscopy for biomarker detection. On-chip is used to separate the biomarkers without introducing any extrinsic contrast agent, which reduces both cost and complexity. The label free detection could be done interrogating separated channel MIR spectroscopy. Phospholipids of degenerative neurological,...
Multiphoton microscopy (MPM) imaging of intrinsic two-photon excited fluorescence (TPEF) is performed on humanized sickle cell disease (SCD) mouse model splenic tissue. Distinct morphological and spectral features associated with SCD are identified discussed in terms diagnostic relevance. Specifically, spectrally unique iron-complex deposits by MPM; this finding supported TPEF spectroscopy object size to standard histopathological methods. Further, iron found at higher concentrations...
Nanoflares are thought to be one of the prime candidates that can heat solar corona its multi-million kelvin temperature. Individual nanoflares difficult detect with present generation instruments, however their presence inferred by comparing simulated nanoflare-heated plasma emissions observed emission. Using HYDRAD coronal loop simulations, we model emission from an X-ray bright point (XBP) Marshall Grazing Incidence Spectrometer (MaGIXS), along nearest-available observations Atmospheric...
The Marshall Grazing Incidence X-ray Spectrometer (MaGIXS) is a sounding rocket experiment that observes the soft spectrum of Sun from 6.0 - 24 Angstrom (0.5 2.0 keV), successfully launched on 30 July 2021. End-to-end alignment flight instrument and calibration experiments are carried out using Cryogenic Facility (XRCF) at NASA Space Flight Center. In this paper, we present MaGIXS, which include wavelength calibration, measurement line spread function, determination effective area. Finally,...
A hydrophobic, oxygen-sensitive ruthenium-complex dye is prepared in water through a simple surfactant micellization technique. Two-photon imaging of mouse brain vasculature vivo demonstrated using the probe with excellent detail and contrast.