A5072456464- Antioxidant Activity and Oxidative Stress
- Plant biochemistry and biosynthesis
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Postharvest Quality and Shelf Life Management
- Bacteriophages and microbial interactions
- dental development and anomalies
- Plant Gene Expression Analysis
- Bone and Dental Protein Studies
- Genomics and Phylogenetic Studies
- Plant Micronutrient Interactions and Effects
- Plant Virus Research Studies
- Reconstructive Facial Surgery Techniques
- Advanced Chemical Sensor Technologies
- Topic Modeling
- Plant tissue culture and regeneration
- Oral and Maxillofacial Pathology
- Reconstructive Surgery and Microvascular Techniques
- Plant nutrient uptake and metabolism
- Metabolomics and Mass Spectrometry Studies
- Plant Pathogenic Bacteria Studies
SRM University, Andhra Pradesh
2023-2024
University of Hyderabad
2015-2024
SRM University
2024
Sawai ManSingh Medical College and Hospital
2020-2023
Hebrew University of Jerusalem
2021-2022
Maulana Azad Institute of Dental Sciences
2017-2020
University of Delaware
2012
Biotechnology Institute
2012
Cornell University
2007-2012
University of Maryland, College Park
2008
The dietary carotenoids serve as precursor for vitamin A and prevent several chronic-degenerative diseases. carotenoid profiling is necessary to understand their importance on human health. However, the available high-performance liquid chromatography (HPLC) methods resolve major require longer analysis times do not adequately violaxanthin neoxanthin.A fast sensitive HPLC method was developed using a C30 column at 20°C with gradient consisting of methanol, methyl-tert-butyl ether water....
The identification of mutations in targeted genes has been significantly simplified by the advent TILLING (Targeting Induced Local Lesions In Genomes), speeding up functional genomic analysis animals and plants. Next-generation sequencing (NGS) is gradually replacing classical for mutation detection, as it allows a large number amplicons short durations. NGS approach was used to identify population Solanum lycopersicum (tomato) that doubly mutagenized ethylmethane sulphonate (EMS)....
Domestication of tomato has resulted in large diversity fruit phenotypes. An intensive phenotyping 127 accessions from 20 countries revealed extensive morphological traits. The traits clustered the into nine classes and identified certain promising lines having desirable pertaining to total soluble salts (TSS), carotenoids, ripening index, weight shape. Factor analysis morphometric data Tomato Analyzer showed that shape is a complex trait shared by several factors. 100% variance between...
Induced mutations accelerate crop improvement by providing novel disease resistance and yield alleles. However, the alleles with no perceptible phenotype but have an altered function remain hidden in mutagenized plants. The whole-genome sequencing (WGS) of individuals uncovers complete spectrum genome. Genome-wide induced mutation resources can improve targeted breeding tomatoes facilitate functional genomics. In this study, we sequenced 132 doubly ethyl methanesulfonate (EMS)-mutagenized...
Programmed ribosomal frameshifting (PRF) is a process by which ribosomes produce two different polypeptides from the same mRNA. In this study, we propose three kinetic models of +1 PRF, incorporating effects E-, P- and A-sites toward promoting efficient in Escherichia coli . Specifically, timing E-site tRNA dissociation discussed within context proofreading mechanism aminoacylated (aa-tRNA) selection. Mathematical modeling using previously determined rate constants reveals that...
Escherichia coli is one of the most widely used hosts for production recombinant proteins. Extracellular protein secretion has advantage reducing aggregation and simplifying downstream purification. The introduction five rare codons in a specific region alpha-hemolysin (hlyA) gene previously was shown to result eightfold improvement HlyA via hemolysin (Type-I) pathway. Here we investigate biological basis observed phenomenon that translation rate may be related ability secrete higher levels...
Abstract Motivation Facilitated by technological advances and expeditious decrease in the sequencing costs, whole-genome is increasingly implemented to uncover variations cultivars/accessions of many crop plants. In tomato (Solanum lycopersicum), availability genome sequence, followed resequencing cultivars its wild relatives, has provided a prodigious resource for improvement traits. A high-quality 84 accessions relatives generated dataset that can be used as identify agronomically...
Background Soft tissue coverage of the distal foot and ankle region has been an area debate due to paucity local flap options. To provide empirical evidence on reliability underreported alternative for defects, we aim compare lateral supramalleolar (LSMF) reverse sural (RSF). Methods During 2016-2019, 48 patients were divided randomly into two equal groups, LSMF RSF groups respectively. The patients' demographic, surgical, clinical outcome details recorded analyzed. Results Flap necrosis was...
Summary The tomato ( Solanum lycopersicum ) ripening inhibitor rin mutation is known to completely repress fruit ripening. heterozygous RIN / fruits have extended shelf life, ripen normally, but inferior taste/flavour. To address this, we used genome editing generate newer alleles of CR by targeting the K‐domain. Unlike previously reported CRISPR alleles, displayed delayed onset ripening, suggesting that mutated K‐domain represses had life and accumulated carotenoids at an intermediate level...
Abstract The induced mutations accelerate crop improvement by providing novel disease resistance and yield alleles. However, the alleles with no perceptible phenotype but having an altered function remain hidden in mutagenized plants. whole-genome sequencing (WGS) of individuals uncovers complete spectrum genome. We sequenced 132 doubly ethyl methanesulfonate (EMS)-mutagenized lines tomato detected ca. 41 million SNPs 5.5 short-INDELs. found a very high average density 1/3.05 Kb compared to...
Abnormal skeletal jaw relationships is an important factor causing difficulty in speech, mastication, sleep and social interaction, thus affect the overall well being of individual.The present study was attempt to decipher role human MSX1 terms sagittal relationship by employing Polymerase Chain Reaction (PCR) based analysis.Ninety-eight case subjects belonging North India with Class II III were selected. Further, thirty-five control same region having I dental (normal Jaw relationships)...
Artificial Intelligence (AI) agents now interact with billions of humans in natural language, thanks to advances Large Language Models (LLMs) like ChatGPT. This raises the question whether AI has potential shape a fundamental aspect human culture: way we speak. Recent analyses revealed that scientific publications already exhibit evidence AI-specific language. But this is inconclusive, since scientists may simply be using copy-edit their writing. To explore influenced spoken communication,...