A5102733303- Extracellular vesicles in disease
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Single-cell and spatial transcriptomics
- RNA and protein synthesis mechanisms
- Molecular Biology Techniques and Applications
Fudan University
2022
Weill Cornell Medicine
2021
Memorial Sloan Kettering Cancer Center
2021
Cornell University
2021
Recent advancement in single-cell RNA sequencing (scRNA-seq) technology is gaining more and attention. Cell type annotation plays an essential role scRNA-seq data analysis. Several computational methods have been proposed for automatic annotation. Traditional cell to first cluster the cells using unsupervised learning based on gene expression profiles, then label clusters aggregated cluster-level profiles marker genes’ information. Such procedure relies heavily clustering results. As purity...
Summary Here we explored the role of minor spliceosome (MiS) function and intron-containing gene (MIG) expression in prostate cancer (PCa). We show MIGs are enriched as direct interactors cancer-causing genes their discriminates PCa progression. Increased MiS U6atac snRNA, including others, 6x more efficient intron splicing was observed castration-resistant (CRPC) versus primary PCa. Notably, androgen receptor signalling influenced activity. Inhibition through siU6atac caused mis-splicing...