A5103059844- Prostate Cancer Treatment and Research
- Bladder and Urothelial Cancer Treatments
- Cancer, Lipids, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Molecular Biology Techniques and Applications
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Tissue Engineering and Regenerative Medicine
- Inhalation and Respiratory Drug Delivery
- 3D Printing in Biomedical Research
- RNA modifications and cancer
- Advanced Radiotherapy Techniques
- Medical Imaging Techniques and Applications
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Urinary and Genital Oncology Studies
- RNA Research and Splicing
- Innovative Microfluidic and Catalytic Techniques Innovation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Chemical Reactions and Isotopes
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Infectious Disease Case Reports and Treatments
- Virus-based gene therapy research
University of Bern
2017-2024
University Hospital of Bern
2018-2024
Suzuki (Japan)
2020
The evaluation of inhalation toxicity, drug safety and efficacy assessment, as well the investigation complex disease pathomechanisms, are increasingly relying on in vitro lung models. This is due to progressive shift towards human-based systems for more predictive translational research. While several cellular models currently available upper airways, modelling distal alveolar region poses constraints that make standardization reliable relatively difficult. In this work, we present a new...
The evolutionarily conserved minor spliceosome (MiS) is required for protein expression of ∼714 intron-containing genes (MIGs) crucial cell-cycle regulation, DNA repair, and MAP-kinase signaling. We explored the role MIGs MiS in cancer, taking prostate cancer (PCa) as an exemplar. Both androgen receptor signaling elevated levels U6atac, a small nuclear RNA, regulate activity, which highest advanced metastatic PCa. siU6atac-mediated inhibition PCa vitro model systems resulted aberrant intron...
Abstract On September 23–24 (2024) the 6th Workshop IRE on Translational Oncology, titled “Cancer Organoids as Reliable Disease Models to Drive Clinical Development of Novel Therapies,” took place at IRCCS Regina Elena Cancer Institute in Rome. This prominent international conference focused tumor organoids, bringing together leading experts from around world. A central challenge precision oncology is modeling dynamic ecosystem, which encompasses numerous elements that evolve spatially and...
Abstract Background Localized prostate cancer (PCa) is a largely heterogeneous disease regarding its clinical behavior. Current risk stratification relies on clinicopathological parameters and distinguishing between indolent aggressive cases remains challenging. To improve stratification, we aimed to identify new prognostic markers for PCa. Methods We performed an in silico analysis publicly available PCa transcriptome datasets. The top 20 genes were assessed tissue samples of our...
Abstract Advanced prostate cancer initially responds to hormonal treatment, but ultimately becomes resistant and requires more potent therapies. One mechanism of resistance observed in ∼10% these patients is through lineage plasticity, which manifests a partial or complete small cell neuroendocrine (NEPC) phenotype. Here, we investigate the role mammalian SWI/SNF (mSWI/SNF) chromatin remodeling complex NEPC. Using large patient datasets, patient-derived organoids lines, identify mSWI/SNF...
We spoke to Prof. Marianna Kruithof-de Julio and her team at the University of Bern about their research which involves using patient-derived organoids improve bladder cancer treatment. Their work aims tailor therapies based on individual tumor profiles, addressing disease’s high heterogeneity recurrence rates
Summary Here we explored the role of minor spliceosome (MiS) function and intron-containing gene (MIG) expression in prostate cancer (PCa). We show MIGs are enriched as direct interactors cancer-causing genes their discriminates PCa progression. Increased MiS U6atac snRNA, including others, 6x more efficient intron splicing was observed castration-resistant (CRPC) versus primary PCa. Notably, androgen receptor signalling influenced activity. Inhibition through siU6atac caused mis-splicing...
Abstract Introduction: Prostate cancer (PCa) is a highly heterogeneous disease with multiple and distinct foci within the same prostate gland. We hypothesize that lesions, may be linked to different functional properties e.g. differential drug response sensitivity. Here we investigate which histopathological molecular are associated ability maintain PCa cells as ex vivo organoid cultures possibly specific responses. Methods: To simultaneously assess histopathology, genetic profile...
In prostate cancer, long-term treatment directed against androgens often leads to the development of metastatic castration-resistant which is more aggressive and not curatively treatable. Androgen deprivation results in elevated epiregulin expression LNCaP cells a ligand EGFR. This study aims reveal regulation different cancer stages enabling specific molecular characterization carcinoma types.Five cell lines were used characterize on RNA protein levels. Epiregulin its correlation with...
Abstract Bladder Cancer (BLCa) inter-patient heterogeneity is considered the primary cause of tumor reoccurrence and treatment failure, suggesting that BLCa patients could benefit from a more personalized approach. Patient-derived organoids (PDOs) have been successfully used as functional model for predicting drug response in different cancer types. In our study, we established PDO cultures stages. PDOs preserve histological molecular parental tumors, including their multiclonal genetic...
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, due to lack of experimental models that mimic different disease stages. We describe a novel androgen-dependent PCa patient-derived xenograft (PDX) model from treatment-naïve, soft tissue metastasis (PNPCa). RNA whole-exome sequencing the PDX organoids confirmed transcriptomic genomic similarity primary tumor. PNPCa harbours BRCA2 CHD1 somatic mutations, shows an SPOP/FOXA1 -like signature...
Summary Synthetic lethal interactions, where the simultaneous but not individual inactivation of two genes is to cell, have been successfully exploited treat cancer. GATA3 frequently mutated in estrogen receptor (ER)-positive breast cancers and its deficiency defines a subset patients with poor response hormonal therapy prognosis. However, targetable. Here we show that MDM2 are synthetically ER-positive Depletion pharmacological inhibition induce apoptosis -deficient models vitro, vivo...
Idiopathic Pulmonary fibrosis (IPF) is an incurable chronic disease characterized by progressive loss of lung function. IPF thought to occur due repetitive micro injuries in the distal and/or genetic predisposition leading aberrant tissue repair. Increased ECM deposition myofibroblasts results stiffening and thickening alveolar walls, causing decline pulmonary function ultimately, fatal respiratory failure. Due inherent complexity human disease, advanced vitro models including physiological...
Driver genes with a mutually exclusive mutation pattern across tumor genomes are thought to have overlapping roles in tumorigenesis. In contrast, we show here that mutually-exclusive prostate cancer driver alterations involving the ERG transcription factor and ubiquitin ligase adaptor SPOP synthetic sick. At molecular level, incompatible pathways driven by opposing functions SPOP. up-regulates wild type dampen androgen receptor (AR) signaling sustain activity through degradation of...
You have accessJournal of UrologyCME1 Apr 2023MP11-05 TUMOR HETEROGENEITY AND DRUG SENSITIVITY MODELLED IN PRIMARY PROSTATE CANCER ORGANOIDS Juening Kang, Sofia Karkampouna, Katja Ovchinnikova, Panagiotis Chouvardas, Marianna Kruithof-de Julio, and George Thalmann KangJuening Kang More articles by this author , KarkampounaSofia Karkampouna OvchinnikovaKatja Ovchinnikova ChouvardasPanagiotis Chouvardas JulioMarianna Julio ThalmannGeorge View All Author...
You have accessJournal of UrologyCME1 Apr 2023MP20-14 GENERATION OF A NEW GEMMs MODEL TO EXPLORE THE ROLE ONCOFETAL CRIPTO IN PROSTATE CANCER PROGRESSION Elisa Rodrigues Sousa, Eugenio Zoni, Mario Scarpa, Marta De Menna, Allen Abey Alexander, Simone Brot, George N. Thalmann, and Marianna Kruithof-de Julio SousaElisa Sousa More articles by this author , ZoniEugenio Zoni ScarpaMario Scarpa MennaMarta Menna AlexanderAllen Alexander BrotSimone Brot ThalmannGeorge Thalmann JulioMarianna View All...
<div>Abstract<p><b>Purpose:</b> To generate a transgenic mouse that when crossed with spontaneous models of lymphoma will allow for quantitative <i>in vivo</i> measurement tumor burden over the entire spectrum disease and or response to therapy in “disease” subtype-specific manner.</p><p><b>Experimental Design:</b> We developed novel genetically engineered using CherryLuciferase fusion gene targeted CD19 locus achieve...
Abstract Introduction: Interpatient heterogeneity is one of the causes treatment failure in bladder cancer (BLCa) patients, who would therefore profit from tailor-made therapies. Toward direction precision medicine, patient-derived organoids (PDOs), which retain parental tumor (PT) features, have been successfully tested different types and shown to predict patients’ responses. Therefore, our study aimed explore ability BLCa PDOs PT features determine patient drug sensitivity. We correlated...