A5045907839- Head and Neck Cancer Studies
- Lung Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Chronic Lymphocytic Leukemia Research
- RNA modifications and cancer
- Radiomics and Machine Learning in Medical Imaging
- Medical Imaging Techniques and Applications
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Advanced Radiotherapy Techniques
- Bioinformatics and Genomic Networks
- Tracheal and airway disorders
- Genetic factors in colorectal cancer
- Esophageal Cancer Research and Treatment
- HER2/EGFR in Cancer Research
- Cell death mechanisms and regulation
- Peptidase Inhibition and Analysis
- Phagocytosis and Immune Regulation
Humboldt-Universität zu Berlin
2017-2025
Charité - Universitätsmedizin Berlin
2016-2025
Freie Universität Berlin
2017-2025
German Cancer Research Center
2016-2025
Heidelberg University
2016-2025
Deutschen Konsortium für Translationale Krebsforschung
2016-2025
German Cancer Society
2016-2024
GTx (United States)
2023
German Marine Research Consortium
2022
Berlin Institute of Health at Charité - Universitätsmedizin Berlin
2018-2021
Abstract Background Circulating tumour cells (CTCs) have shown prognostic relevance in metastatic breast, prostate, colon and pancreatic cancer. For further development of CTCs as a biomarker, we compared the performance different protocols for CTC detection murine breast cancer xenograft models (MDA-MB-231, MDA-MB-468 KPL-4). Blood samples were taken from bearing animals (20 to 200 mm 2 ) analysed using 1. an epithelial marker based enrichment method (AdnaTest), 2. antibody independent...
Abstract Background A limitation of positive selection strategies to enrich for circulating tumor cells (CTCs) is that there might be CTCs with insufficient expression the surface target marker which may missed by procedure. We optimized a method enrichment, subsequent detection and characterization based on depletion leukocyte fraction. Methods The 2-step protocol was developed processing 20 mL blood red cell lysis followed depletion. remaining material stained epithelial markers EpCAM...
Constitutive activation of epidermal growth factor receptor (EGFR) as a result gene amplification, mutation, or overexpression its ligands has been associated with response to EGFR targeting strategies. The role these molecular mechanisms for the responsiveness squamous cell carcinoma head and neck (SCCHN) cetuximab-containing regimens remains unknown.Tumor biopsies from 47 patients, enrolled in single-arm phase II multicenter study second-line treatment recurrent metastatic SCCHN cetuximab...
// Evelyn Kidess-Sigal 1, 2, * , Haiyan E. Liu 3, Melanie M. Triboulet 2 James Che 3 Vishnu C. Ramani Brendan Visser George A. Poultsides Teri Longacre 4 Andre Marziali 5 Valentina Vysotskaia 6 Matthew Wiggin Kyra Heirich Violet Hanft Ulrich Keilholz 7 Ingeborg Tinhofer 8 Jeffrey Norton Mark Lee 9 Elodie Sollier-Christen Stefanie S. 1 Department of Medicine, Division Hepatology and Gastroenterology, Charité University Hospital, Berlin, Germany Surgery, Stanford School Stanford, CA, USA...
Cetuximab, a monoclonal antibody targeting epidermal growth factor receptor (EGFR), has shown clinical efficacy in squamous cell carcinoma of the head and neck with prolonged progression-free (PFS) overall survival (OS). In this study, we analyzed whether cetuximab-induced skin rash was correlated distinct polymorphisms within EGFR gene known to modulate expression, ligand binding, or signaling activity.Fifty-one patients enrolled single-arm phase II multicenter study for second-line...
Purpose: Platinum-based drugs, in particular cisplatin (cis-diamminedichloridoplatinum(II), CDDP), are used for treatment of squamous cell carcinoma the head and neck (SCCHN). Despite initial responses, CDDP often results chemoresistance, leading to therapeutic failure. The role primary resistance at subclonal level treatment-induced clonal selection development remains unknown.Experimental Design: By applying targeted next-generation sequencing, fluorescence situ hybridization,...
Abstract Human papilloma virus-negative head and neck squamous cell carcinoma (HNSCC) frequently harbors 11q13 amplifications. Among the oncogenes at this locus, CCND1 ANO1 are linked to poor prognosis; however, their individual roles in treatment resistance remain unclear. The impact of Cyclin D1 Ano1 overexpression on survival was analyzed using TCGA HNSCC dataset a Charité cohort treated with cisplatin (CDDP)-based radiochemotherapy. High expression primarily associated overall both...
Abstract Ionising radiation causes the introduction of DNA damage, more specifically double strand breaks (DSBs) and complex damage (CDD), that induces cancer cell death leading to therapeutic effect. To combat this, cells activate arrest at G 2 /M checkpoint allow for effective repair, coordinated by Chk1 Wee1 protein kinases. Therefore, are considered promising targets enhance effectiveness radiotherapy in killing. Here, we have analysed response head neck squamous carcinoma (HNSCC) lines,...
Abstract Circulating tumor cells (CTCs) might not only serve as prognostic marker but could also be useful for monitoring treatment efficacy. A multicolor flow cytometry protocol their detection and molecular characterization in peripheral blood was developed which consisted of erythrocyte lysis followed by staining with fluorochrome‐labeled antibodies against CD45 the epithelial markers EpCam cytokeratin 7/8. For reducing number events acquired cytometry, an electronic threshold fluorescent...