A5034632119- Prostate Cancer Treatment and Research
- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Cellular Mechanics and Interactions
- Bone health and treatments
- Hippo pathway signaling and YAP/TAZ
- SARS-CoV-2 and COVID-19 Research
- Heat shock proteins research
- Circular RNAs in diseases
- MicroRNA in disease regulation
- interferon and immune responses
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Cancer Research and Treatments
- Spaceflight effects on biology
- Urinary and Genital Oncology Studies
- Urological Disorders and Treatments
- Metastasis and carcinoma case studies
- Cancer Cells and Metastasis
- Computational Drug Discovery Methods
- Cancer-related gene regulation
- Radiopharmaceutical Chemistry and Applications
- Wnt/β-catenin signaling in development and cancer
- Cancer-related molecular mechanisms research
- PARP inhibition in cancer therapy
University of Bern
2018-2024
University Hospital of Bern
2018-2022
University of Naples Federico II
2012-2021
Suzuki (Japan)
2020
Magna Graecia University
2016
Zero to Three
2016
European School of Molecular Medicine
2010
Stazione Zoologica Anton Dohrn
2004
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, due to lack of experimental models that mimic different disease stages. We describe an androgen-dependent PCa patient-derived xenograft (PDX) model from treatment-naïve, soft tissue metastasis (PNPCa). RNA whole-exome sequencing the PDX organoids confirmed transcriptomic genomic similarity primary tumor. PNPCa harbors BRCA2 CHD1 somatic mutations, shows SPOP/FOXA1 -like signature microsatellite...
Pancreatic cancer (PDAC) is a highly aggressive malignancy for which the identification of novel therapies urgently needed. Here, we establish human PDAC organoid biobank from 31 genetically distinct lines, covering representative range tumor subtypes, and demonstrate that these reflect molecular phenotypic heterogeneity primary tissue. We use CRISPR-Cas9 genome editing drug screening to characterize drug-gene interactions with ARID1A BRCA2. find missense- but not frameshift mutations in...
Activating mutations in RAS protooncogenes are associated with several different histotypes of thyroid cancer, including anaplastic carcinoma. The latter is the most aggressive cancer gland, showing little or no expression differentiated phenotype. Likewise, viral oncogenes FRTL-5 rat cells mimics such loss differentiation. We established cell lines stably expressing constitutively active forms RAS, either (v-Ha-RAS v-Ki-RAS) cellular (H-RASV12) origin and generated a tamoxifen-inducible...
Bone metastasis is the leading cause of prostate cancer (PCa) mortality, frequently marking progression to castration-resistant PCa. Dysregulation androgen receptor pathway a common feature PCa, appearing in association with mTOR deregulations. Advanced PCa also characterized by increased tumor heterogeneity and stem cell (CSC) frequency. CSC-targeted therapy currently being explored advanced aim reducing clonal divergence preventing disease progression. In this study, we compared molecular...
Synthetic lethal interactions, where the simultaneous but not individual inactivation of two genes is to cell, have been successfully exploited treat cancer. GATA3 frequently mutated in estrogen receptor (ER)-positive breast cancers and its deficiency defines a subset patients with poor response hormonal therapy prognosis. However, yet targetable. Here we show that MDM2 are synthetically ER-positive Depletion pharmacological inhibition significantly impaired tumor growth GATA3-deficient...
Alterations in the cytoskeleton structure are frequently found several diseases and particularly cancer cells. It is also through alterations of that cells acquire most their common features such as uncontrolled cell proliferation, death evasion, gaining migratory invasive characteristics. Although radiation therapies currently represent one effective treatments for patients, effects X-irradiation on architecture still poorly understood. In this case we investigated effects, over time two...
In intermediate-risk non-muscle invasive bladder cancer (NMIBC) clinical guidelines suggest an adjuvant instillation with a chemotherapeutic agent. However, the agent and regimen are not clearly defined. Worldwide, less than 15% of patients receive this instillation. We recently developed pipeline for generation patient derived organoids (PDO) in NMIBC. phase II trial, we aim to use our vitro select most effective drug NMIBC patients.Patients first diagnosis that directed transurethral...
Cytoskeleton plays a central role in many cellular processes, such as migration, adhesion and proliferation. Alterations of its structural properties are commonly associated with different diseases (malignancy, cardiac hypertrophy, etc.). In this work, we studied the effects X-radiations on cytoskeleton architecture two cell lines: BALBc/3T3 Simian virus 40-transformed (SVT2) cells. agreement current literature, observed reduced increased motility SVT2 cells respect to non-transformed...
Abstract The regulatory transcriptional factor PATZ1 is constantly downregulated in human thyroid cancer where it acts as a tumour suppressor by targeting p53-dependent genes involved Epithelial-Mesenchymal Transition and cell migration. aim of the present work was to elucidate upstream signalling mechanisms regulating expression cells. bioinformatics search for microRNAs able potentially target led identification several miRNAs. Among them we focused on miR-29b since found upregulated rat...
SARS-CoV-2 hijacks the host cell transcriptional machinery to induce a phenotypic state amenable its replication. Here we show that analysis of Master Regulator proteins representing mechanistic determinants gene expression signature induced by in infected cells revealed coordinated inactivation Regulators enriched physical interactions with proteins, suggesting their role maintaining refractory virus To test functional relevance, measured replication epithelial treated drugs predicted...
The mechanobiology is providing novel perspectives in the study of cancer and contributing to evaluate responses, from a biophysical point view, classical therapeutic approaches- radiotherapy chemotherapy. Here we have explored effects two doses (4 8 Gy) 6 MeV photons on spreading, focal adhesions, migration mechanical properties BALB/c 3T3 their SV40 transformed equivalent, SVT2. Cell responses 4 Gy were analysed compared with those reported previous published work when lower (1 2...
TPS711 Background: Recurrence rates in intermediate risk non-muscle invasive bladder cancer (NMIBC) are high and therefore, clinical guidelines recommend adjuvant instillations. However, the exact regimen with an number of instillations chemotherapeutic agent to be used not clearly defined. During last years we have established a standardized pipeline generate patient-derived organoids (PDO) from cold cup biopsies NMIBC. We showed that subsequent maintain key features parental tumor share...
Abstract Background: Androgen deprivation therapy is the standard treatment for prostate cancer (PCa). Nevertheless, despite initial effectiveness, pre-existing stem cell (CSC) populations invariably lead to incurable castration-resistant cancer. CSCs are a subset of cells possessing self-renewal properties, driving tumor progression and regrowth. CD44+ PCa exhibit more stemness features enriched in tumorigenic metastatic progenitor cells. Here, we aim explore different subpopulations tumors...
Abstract CRIPTO (or CR-1 or TDGF1) is a protein that plays an active role in tumor initiation and progression. We have confirmed increased expression of associated with clinical prostate-specific antigen (PSA) progression human prostate tissues. Our approach involved gaining insight into the signaling castration-resistant Nkx3.1- expressing cells (CARNs), targets for oncogenic transformation cancer (PCa), by integrating existing Cripto flox/flox CARNs model. The most aggressive stage was...
Abstract Therapy resistance and metastatic processes in prostate cancer (PCa) remain undefined, due to lack of experimental models that mimic different disease stages. We describe a novel androgen-dependent PCa patient-derived xenograft (PDX) model from treatment-naïve, soft tissue metastasis (PNPCa). RNA whole-exome sequencing the PDX organoids confirmed transcriptomic genomic similarity primary tumor. PNPCa harbours BRCA2 CHD1 somatic mutations, shows an SPOP/FOXA1 -like signature...
Summary Synthetic lethal interactions, where the simultaneous but not individual inactivation of two genes is to cell, have been successfully exploited treat cancer. GATA3 frequently mutated in estrogen receptor (ER)-positive breast cancers and its deficiency defines a subset patients with poor response hormonal therapy prognosis. However, targetable. Here we show that MDM2 are synthetically ER-positive Depletion pharmacological inhibition induce apoptosis -deficient models vitro, vivo...
Forkhead box E1 (FOXE1) is a lineage-restricted transcription factor involved in thyroid cancer susceptibility. Cancer-associated polymorphisms map regulatory regions, thus affecting the extent of gene expression. We have recently shown that genetic reduction FOXE1 dosage modifies multiple phenotypes. To identify relevant effectors playing roles development, here we analyse FOXE1-induced transcriptional alterations cells do not express endogenous FOXE1. Expression elicits cell migration,...